Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11220 and PS417_11225 overlap by 4 nucleotides PS417_11225 and PS417_11230 are separated by 147 nucleotides
PS417_11220: PS417_11220 - signal peptide protein, at 2,405,546 to 2,406,937
_11220
PS417_11225: PS417_11225 - glyoxalase, at 2,406,934 to 2,407,830
_11225
PS417_11230: PS417_11230 - hydroxyacid dehydrogenase, at 2,407,978 to 2,409,045
_11230
Position (kb)
2406
2407
2408 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2406.013 kb on + strand at 2406.016 kb on - strand, within PS417_11220 at 2406.171 kb on + strand, within PS417_11220 at 2406.192 kb on + strand, within PS417_11220 at 2406.201 kb on + strand, within PS417_11220 at 2406.201 kb on + strand, within PS417_11220 at 2406.559 kb on - strand, within PS417_11220 at 2406.646 kb on - strand, within PS417_11220 at 2406.766 kb on - strand, within PS417_11220 at 2406.766 kb on - strand, within PS417_11220 at 2407.279 kb on - strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.625 kb on - strand, within PS417_11225 at 2407.625 kb on - strand, within PS417_11225 at 2407.706 kb on + strand, within PS417_11225 at 2407.724 kb on + strand, within PS417_11225 at 2407.932 kb on - strand at 2407.936 kb on + strand at 2407.963 kb on - strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.991 kb on - strand at 2407.996 kb on + strand at 2408.069 kb on + strand at 2408.324 kb on - strand, within PS417_11230 at 2408.324 kb on - strand, within PS417_11230 at 2408.470 kb on + strand at 2408.471 kb on - strand, within PS417_11230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,406,013 + -0.2 2,406,016 - PS417_11220 0.34 -0.5 2,406,171 + PS417_11220 0.45 -0.6 2,406,192 + PS417_11220 0.46 -0.6 2,406,201 + PS417_11220 0.47 +0.4 2,406,201 + PS417_11220 0.47 +1.0 2,406,559 - PS417_11220 0.73 +0.2 2,406,646 - PS417_11220 0.79 -1.0 2,406,766 - PS417_11220 0.88 -0.1 2,406,766 - PS417_11220 0.88 -0.5 2,407,279 - PS417_11225 0.38 +1.0 2,407,624 + PS417_11225 0.77 +1.4 2,407,624 + PS417_11225 0.77 +0.3 2,407,624 + PS417_11225 0.77 +1.4 2,407,625 - PS417_11225 0.77 -0.8 2,407,625 - PS417_11225 0.77 -2.4 2,407,706 + PS417_11225 0.86 -0.3 2,407,724 + PS417_11225 0.88 +2.9 2,407,932 - +0.2 2,407,936 + +2.6 2,407,963 - -0.0 2,407,976 + +0.3 2,407,976 + +0.6 2,407,976 + -0.5 2,407,976 + +0.0 2,407,991 - -0.0 2,407,996 + -0.2 2,408,069 + -0.5 2,408,324 - PS417_11230 0.32 -0.8 2,408,324 - PS417_11230 0.32 -0.9 2,408,470 + -0.2 2,408,471 - PS417_11230 0.46 -0.4
Or see this region's nucleotide sequence