Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10825 and PS417_10830 are separated by 6 nucleotides PS417_10830 and PS417_10835 are separated by 136 nucleotides
PS417_10825: PS417_10825 - glycine/betaine ABC transporter substrate-binding protein, at 2,314,492 to 2,315,391
_10825
PS417_10830: PS417_10830 - LysR family transcriptional regulator, at 2,315,398 to 2,316,315
_10830
PS417_10835: PS417_10835 - major facilitator transporter, at 2,316,452 to 2,317,768
_10835
Position (kb)
2315
2316
2317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2314.398 kb on + strand at 2314.795 kb on + strand, within PS417_10825 at 2314.796 kb on - strand, within PS417_10825 at 2314.796 kb on - strand, within PS417_10825 at 2314.829 kb on - strand, within PS417_10825 at 2314.829 kb on - strand, within PS417_10825 at 2314.996 kb on + strand, within PS417_10825 at 2315.012 kb on - strand, within PS417_10825 at 2315.399 kb on + strand at 2315.399 kb on + strand at 2316.451 kb on + strand at 2316.452 kb on - strand at 2316.851 kb on + strand, within PS417_10835 at 2316.851 kb on + strand, within PS417_10835 at 2316.852 kb on - strand, within PS417_10835 at 2316.854 kb on + strand, within PS417_10835 at 2316.854 kb on + strand, within PS417_10835 at 2316.869 kb on + strand, within PS417_10835 at 2317.055 kb on + strand, within PS417_10835 at 2317.055 kb on + strand, within PS417_10835 at 2317.056 kb on - strand, within PS417_10835 at 2317.165 kb on + strand, within PS417_10835 at 2317.188 kb on + strand, within PS417_10835 at 2317.188 kb on + strand, within PS417_10835 at 2317.189 kb on - strand, within PS417_10835 at 2317.189 kb on - strand, within PS417_10835 at 2317.190 kb on + strand, within PS417_10835 at 2317.191 kb on - strand, within PS417_10835 at 2317.232 kb on + strand, within PS417_10835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,314,398 + +2.7 2,314,795 + PS417_10825 0.34 +1.3 2,314,796 - PS417_10825 0.34 +0.2 2,314,796 - PS417_10825 0.34 -0.7 2,314,829 - PS417_10825 0.37 +0.7 2,314,829 - PS417_10825 0.37 +0.1 2,314,996 + PS417_10825 0.56 -0.6 2,315,012 - PS417_10825 0.58 +0.2 2,315,399 + -0.8 2,315,399 + +0.6 2,316,451 + -0.3 2,316,452 - +0.4 2,316,851 + PS417_10835 0.30 +0.0 2,316,851 + PS417_10835 0.30 +0.1 2,316,852 - PS417_10835 0.30 +0.1 2,316,854 + PS417_10835 0.31 +1.1 2,316,854 + PS417_10835 0.31 +0.1 2,316,869 + PS417_10835 0.32 -0.9 2,317,055 + PS417_10835 0.46 +1.9 2,317,055 + PS417_10835 0.46 -0.0 2,317,056 - PS417_10835 0.46 +0.3 2,317,165 + PS417_10835 0.54 -3.0 2,317,188 + PS417_10835 0.56 +0.3 2,317,188 + PS417_10835 0.56 -2.0 2,317,189 - PS417_10835 0.56 -1.4 2,317,189 - PS417_10835 0.56 +2.0 2,317,190 + PS417_10835 0.56 +1.5 2,317,191 - PS417_10835 0.56 -0.6 2,317,232 + PS417_10835 0.59 -0.1
Or see this region's nucleotide sequence