Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10630 and PS417_10635 are separated by 397 nucleotides PS417_10635 and PS417_10640 are separated by 12 nucleotides PS417_10640 and PS417_10645 are separated by 421 nucleotides
PS417_10630: PS417_10630 - hydratase, at 2,268,160 to 2,269,047
_10630
PS417_10635: PS417_10635 - peptidylprolyl isomerase, at 2,269,445 to 2,269,789
_10635
PS417_10640: PS417_10640 - GNAT family acetyltransferase, at 2,269,802 to 2,270,404
_10640
PS417_10645: PS417_10645 - magnesium ABC transporter ATPase, at 2,270,826 to 2,273,531
_10645
Position (kb)
2269
2270
2271 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2268.806 kb on - strand, within PS417_10630 at 2268.882 kb on - strand, within PS417_10630 at 2269.061 kb on + strand at 2269.061 kb on + strand at 2269.061 kb on + strand at 2269.061 kb on + strand at 2269.061 kb on + strand at 2269.062 kb on - strand at 2269.062 kb on - strand at 2269.067 kb on + strand at 2269.070 kb on - strand at 2269.094 kb on + strand at 2269.096 kb on + strand at 2269.096 kb on + strand at 2269.116 kb on - strand at 2269.116 kb on - strand at 2269.147 kb on + strand at 2269.148 kb on - strand at 2269.161 kb on + strand at 2269.161 kb on + strand at 2269.176 kb on + strand at 2269.177 kb on - strand at 2269.228 kb on + strand at 2269.231 kb on + strand at 2269.231 kb on + strand at 2269.231 kb on + strand at 2269.232 kb on - strand at 2269.317 kb on + strand at 2269.317 kb on + strand at 2269.529 kb on + strand, within PS417_10635 at 2269.530 kb on - strand, within PS417_10635 at 2269.665 kb on + strand, within PS417_10635 at 2269.665 kb on + strand, within PS417_10635 at 2269.666 kb on - strand, within PS417_10635 at 2269.666 kb on - strand, within PS417_10635 at 2269.669 kb on + strand, within PS417_10635 at 2269.669 kb on + strand, within PS417_10635 at 2269.669 kb on + strand, within PS417_10635 at 2269.670 kb on - strand, within PS417_10635 at 2269.916 kb on + strand at 2269.917 kb on - strand, within PS417_10640 at 2270.012 kb on + strand, within PS417_10640 at 2270.012 kb on + strand, within PS417_10640 at 2270.013 kb on - strand, within PS417_10640 at 2270.262 kb on - strand, within PS417_10640 at 2270.271 kb on + strand at 2270.272 kb on - strand, within PS417_10640 at 2270.360 kb on + strand at 2270.360 kb on + strand at 2270.360 kb on + strand at 2270.361 kb on - strand at 2270.361 kb on - strand at 2270.457 kb on - strand at 2270.477 kb on + strand at 2270.478 kb on - strand at 2270.520 kb on + strand at 2270.520 kb on + strand at 2270.538 kb on + strand at 2270.538 kb on + strand at 2270.539 kb on - strand at 2270.710 kb on + strand at 2270.758 kb on + strand at 2270.761 kb on - strand at 2271.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,268,806 - PS417_10630 0.73 -0.3 2,268,882 - PS417_10630 0.81 +0.6 2,269,061 + +0.1 2,269,061 + -0.0 2,269,061 + -0.2 2,269,061 + +0.6 2,269,061 + +2.2 2,269,062 - +0.2 2,269,062 - +0.8 2,269,067 + +0.2 2,269,070 - -0.9 2,269,094 + -0.4 2,269,096 + -0.5 2,269,096 + -0.6 2,269,116 - -0.3 2,269,116 - -0.2 2,269,147 + -1.4 2,269,148 - -1.6 2,269,161 + +1.5 2,269,161 + -1.4 2,269,176 + -0.4 2,269,177 - +0.1 2,269,228 + -0.5 2,269,231 + -0.0 2,269,231 + -0.7 2,269,231 + +0.4 2,269,232 - +1.2 2,269,317 + -0.9 2,269,317 + +1.6 2,269,529 + PS417_10635 0.24 +0.5 2,269,530 - PS417_10635 0.25 +0.1 2,269,665 + PS417_10635 0.64 +0.6 2,269,665 + PS417_10635 0.64 -3.9 2,269,666 - PS417_10635 0.64 -0.1 2,269,666 - PS417_10635 0.64 -2.6 2,269,669 + PS417_10635 0.65 -1.3 2,269,669 + PS417_10635 0.65 +0.1 2,269,669 + PS417_10635 0.65 +0.3 2,269,670 - PS417_10635 0.65 +0.1 2,269,916 + -0.6 2,269,917 - PS417_10640 0.19 +2.7 2,270,012 + PS417_10640 0.35 +1.1 2,270,012 + PS417_10640 0.35 -0.7 2,270,013 - PS417_10640 0.35 +0.5 2,270,262 - PS417_10640 0.76 -2.7 2,270,271 + -1.8 2,270,272 - PS417_10640 0.78 +0.5 2,270,360 + -0.9 2,270,360 + +0.4 2,270,360 + +0.8 2,270,361 - +0.9 2,270,361 - -0.2 2,270,457 - +0.5 2,270,477 + -1.1 2,270,478 - -0.2 2,270,520 + +0.4 2,270,520 + -0.4 2,270,538 + +0.5 2,270,538 + -0.2 2,270,539 - +1.8 2,270,710 + +0.3 2,270,758 + -0.3 2,270,761 - -1.2 2,271,072 + +1.4
Or see this region's nucleotide sequence