Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10560 and PS417_10565 are separated by 128 nucleotides PS417_10565 and PS417_10570 are separated by 5 nucleotides
PS417_10560: PS417_10560 - choline dehydrogenase, at 2,253,706 to 2,255,352
_10560
PS417_10565: PS417_10565 - LysR family transcriptional regulator, at 2,255,481 to 2,256,374
_10565
PS417_10570: PS417_10570 - cysteine protease, at 2,256,380 to 2,258,239
_10570
Position (kb)
2255
2256
2257 Strain fitness (log2 ratio)
-2
-1
0
1 at 2254.504 kb on + strand, within PS417_10560 at 2254.505 kb on - strand, within PS417_10560 at 2254.505 kb on - strand, within PS417_10560 at 2254.525 kb on - strand, within PS417_10560 at 2254.765 kb on + strand, within PS417_10560 at 2255.034 kb on - strand, within PS417_10560 at 2255.057 kb on + strand, within PS417_10560 at 2255.057 kb on + strand, within PS417_10560 at 2255.058 kb on - strand, within PS417_10560 at 2255.058 kb on - strand, within PS417_10560 at 2255.193 kb on - strand at 2255.324 kb on + strand at 2255.325 kb on - strand at 2255.325 kb on - strand at 2255.340 kb on + strand at 2255.388 kb on + strand at 2255.388 kb on + strand at 2255.389 kb on - strand at 2255.481 kb on - strand at 2255.973 kb on + strand, within PS417_10565 at 2255.988 kb on + strand, within PS417_10565 at 2255.988 kb on + strand, within PS417_10565 at 2255.989 kb on - strand, within PS417_10565 at 2255.989 kb on - strand, within PS417_10565 at 2256.051 kb on + strand, within PS417_10565 at 2256.318 kb on + strand at 2256.553 kb on - strand at 2256.621 kb on + strand, within PS417_10570 at 2256.621 kb on + strand, within PS417_10570 at 2256.622 kb on - strand, within PS417_10570 at 2256.622 kb on - strand, within PS417_10570 at 2256.622 kb on - strand, within PS417_10570 at 2256.686 kb on - strand at 2256.724 kb on - strand, within PS417_10570 at 2256.724 kb on - strand, within PS417_10570 at 2256.729 kb on + strand, within PS417_10570 at 2256.729 kb on + strand, within PS417_10570 at 2256.729 kb on + strand, within PS417_10570 at 2256.729 kb on + strand, within PS417_10570 at 2256.730 kb on - strand, within PS417_10570 at 2256.730 kb on - strand, within PS417_10570 at 2256.730 kb on - strand, within PS417_10570 at 2256.784 kb on + strand, within PS417_10570 at 2257.030 kb on + strand, within PS417_10570 at 2257.031 kb on - strand, within PS417_10570 at 2257.205 kb on + strand, within PS417_10570 at 2257.350 kb on + strand, within PS417_10570 at 2257.351 kb on - strand, within PS417_10570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,254,504 + PS417_10560 0.48 -0.8 2,254,505 - PS417_10560 0.49 -0.1 2,254,505 - PS417_10560 0.49 +0.2 2,254,525 - PS417_10560 0.50 -1.6 2,254,765 + PS417_10560 0.64 +1.1 2,255,034 - PS417_10560 0.81 +0.6 2,255,057 + PS417_10560 0.82 +1.2 2,255,057 + PS417_10560 0.82 -0.2 2,255,058 - PS417_10560 0.82 +0.8 2,255,058 - PS417_10560 0.82 -0.1 2,255,193 - -1.4 2,255,324 + -0.6 2,255,325 - +1.5 2,255,325 - -0.7 2,255,340 + +0.1 2,255,388 + +0.4 2,255,388 + -0.6 2,255,389 - -0.2 2,255,481 - -0.4 2,255,973 + PS417_10565 0.55 -2.0 2,255,988 + PS417_10565 0.57 -2.5 2,255,988 + PS417_10565 0.57 -0.0 2,255,989 - PS417_10565 0.57 +0.1 2,255,989 - PS417_10565 0.57 -0.4 2,256,051 + PS417_10565 0.64 -0.2 2,256,318 + -0.2 2,256,553 - +0.3 2,256,621 + PS417_10570 0.13 -0.5 2,256,621 + PS417_10570 0.13 +0.1 2,256,622 - PS417_10570 0.13 +0.7 2,256,622 - PS417_10570 0.13 -1.7 2,256,622 - PS417_10570 0.13 +0.0 2,256,686 - +0.4 2,256,724 - PS417_10570 0.18 -0.2 2,256,724 - PS417_10570 0.18 -0.4 2,256,729 + PS417_10570 0.19 -0.1 2,256,729 + PS417_10570 0.19 +0.2 2,256,729 + PS417_10570 0.19 -1.8 2,256,729 + PS417_10570 0.19 +1.0 2,256,730 - PS417_10570 0.19 +0.5 2,256,730 - PS417_10570 0.19 +0.1 2,256,730 - PS417_10570 0.19 +0.1 2,256,784 + PS417_10570 0.22 +0.0 2,257,030 + PS417_10570 0.35 -0.4 2,257,031 - PS417_10570 0.35 +0.2 2,257,205 + PS417_10570 0.44 -0.0 2,257,350 + PS417_10570 0.52 -1.1 2,257,351 - PS417_10570 0.52 -0.0
Or see this region's nucleotide sequence