Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10380 and PS417_10385 are separated by 96 nucleotides PS417_10385 and PS417_10390 are separated by 35 nucleotides PS417_10390 and PS417_10395 are separated by 79 nucleotides
PS417_10380: PS417_10380 - catalase, at 2,220,706 to 2,222,139
_10380
PS417_10385: PS417_10385 - peroxiredoxin, at 2,222,236 to 2,222,652
_10385
PS417_10390: PS417_10390 - RNA polymerase subunit sigma24, at 2,222,688 to 2,223,059
_10390
PS417_10395: PS417_10395 - amidase, at 2,223,139 to 2,223,918
_10395
Position (kb)
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2221.236 kb on - strand, within PS417_10380 at 2221.236 kb on - strand, within PS417_10380 at 2221.334 kb on + strand, within PS417_10380 at 2221.412 kb on + strand, within PS417_10380 at 2221.412 kb on + strand, within PS417_10380 at 2221.554 kb on + strand, within PS417_10380 at 2221.555 kb on - strand, within PS417_10380 at 2221.576 kb on - strand, within PS417_10380 at 2221.576 kb on - strand, within PS417_10380 at 2222.129 kb on - strand at 2222.215 kb on + strand at 2222.216 kb on - strand at 2222.285 kb on + strand, within PS417_10385 at 2222.523 kb on + strand, within PS417_10385 at 2222.627 kb on + strand at 2222.690 kb on - strand at 2222.690 kb on - strand at 2222.691 kb on + strand at 2222.691 kb on + strand at 2222.691 kb on + strand at 2222.692 kb on - strand at 2222.736 kb on - strand, within PS417_10390 at 2222.763 kb on - strand at 2222.908 kb on + strand, within PS417_10390 at 2222.909 kb on - strand, within PS417_10390 at 2223.192 kb on + strand at 2223.357 kb on + strand, within PS417_10395 at 2223.358 kb on - strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,221,236 - PS417_10380 0.37 +0.5 2,221,236 - PS417_10380 0.37 +0.1 2,221,334 + PS417_10380 0.44 +0.8 2,221,412 + PS417_10380 0.49 -0.2 2,221,412 + PS417_10380 0.49 -0.5 2,221,554 + PS417_10380 0.59 +0.4 2,221,555 - PS417_10380 0.59 +0.1 2,221,576 - PS417_10380 0.61 -0.8 2,221,576 - PS417_10380 0.61 -0.4 2,222,129 - -2.2 2,222,215 + +0.3 2,222,216 - +0.5 2,222,285 + PS417_10385 0.12 -0.5 2,222,523 + PS417_10385 0.69 -1.4 2,222,627 + -0.8 2,222,690 - +0.1 2,222,690 - -0.9 2,222,691 + +0.4 2,222,691 + -0.7 2,222,691 + +0.8 2,222,692 - -2.2 2,222,736 - PS417_10390 0.13 -0.4 2,222,763 - -0.6 2,222,908 + PS417_10390 0.59 -0.6 2,222,909 - PS417_10390 0.59 +0.4 2,223,192 + +0.8 2,223,357 + PS417_10395 0.28 +0.4 2,223,358 - PS417_10395 0.28 -1.3 2,223,545 + PS417_10395 0.52 +2.4 2,223,545 + PS417_10395 0.52 +1.8 2,223,546 - PS417_10395 0.52 -0.3 2,223,546 - PS417_10395 0.52 -0.4
Or see this region's nucleotide sequence