Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01005 and PS417_01015 are separated by 484 nucleotides PS417_01015 and PS417_01020 overlap by 20 nucleotides PS417_01020 and PS417_01025 overlap by 4 nucleotides
PS417_01005: PS417_01005 - methionine ABC transporter substrate-binding protein, at 220,602 to 221,384
_01005
PS417_01015: PS417_01015 - polar amino acid ABC transporter permease, at 221,869 to 222,534
_01015
PS417_01020: PS417_01020 - glutamine ABC transporter permease, at 222,515 to 223,174
_01020
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
Position (kb) 221
222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 221.181 kb on + strand, within PS417_01005 at 221.387 kb on + strand at 221.425 kb on - strand at 221.425 kb on - strand at 221.588 kb on - strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.844 kb on + strand at 221.889 kb on + strand at 221.889 kb on + strand at 222.040 kb on + strand, within PS417_01015 at 222.091 kb on + strand, within PS417_01015 at 222.385 kb on + strand, within PS417_01015 at 222.400 kb on + strand, within PS417_01015 at 222.400 kb on + strand, within PS417_01015 at 222.401 kb on - strand, within PS417_01015 at 222.412 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.424 kb on + strand, within PS417_01015 at 222.424 kb on + strand, within PS417_01015 at 222.589 kb on + strand, within PS417_01020 at 222.636 kb on - strand, within PS417_01020 at 222.689 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.732 kb on - strand, within PS417_01020 at 222.917 kb on + strand, within PS417_01020 at 222.923 kb on + strand, within PS417_01020 at 222.924 kb on - strand, within PS417_01020 at 223.067 kb on + strand, within PS417_01020 at 223.067 kb on + strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.086 kb on - strand, within PS417_01020 at 223.114 kb on + strand at 223.115 kb on - strand at 223.435 kb on + strand, within PS417_01025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 221,181 + PS417_01005 0.74 +1.0 221,387 + +0.4 221,425 - -1.1 221,425 - +2.3 221,588 - +0.3 221,694 + -1.9 221,694 + -0.6 221,694 + +0.5 221,694 + +0.6 221,695 - -3.1 221,695 - -1.1 221,695 - +0.7 221,695 - +0.1 221,844 + -0.6 221,889 + -2.1 221,889 + +0.4 222,040 + PS417_01015 0.26 +0.3 222,091 + PS417_01015 0.33 -1.0 222,385 + PS417_01015 0.77 +0.2 222,400 + PS417_01015 0.80 +0.0 222,400 + PS417_01015 0.80 -0.6 222,401 - PS417_01015 0.80 +0.0 222,412 + PS417_01015 0.82 +0.7 222,415 + PS417_01015 0.82 +1.6 222,415 + PS417_01015 0.82 -0.2 222,415 + PS417_01015 0.82 -1.6 222,416 - PS417_01015 0.82 -0.7 222,416 - PS417_01015 0.82 -1.7 222,416 - PS417_01015 0.82 +1.4 222,424 + PS417_01015 0.83 +2.6 222,424 + PS417_01015 0.83 +2.9 222,589 + PS417_01020 0.11 +0.4 222,636 - PS417_01020 0.18 +0.9 222,689 + PS417_01020 0.26 +1.6 222,731 + PS417_01020 0.33 -0.0 222,731 + PS417_01020 0.33 +1.8 222,731 + PS417_01020 0.33 +0.1 222,732 - PS417_01020 0.33 +0.3 222,917 + PS417_01020 0.61 +0.9 222,923 + PS417_01020 0.62 -0.4 222,924 - PS417_01020 0.62 -1.3 223,067 + PS417_01020 0.84 -1.3 223,067 + PS417_01020 0.84 -0.7 223,068 - PS417_01020 0.84 +0.9 223,068 - PS417_01020 0.84 +2.1 223,086 - PS417_01020 0.87 +2.6 223,114 + -0.0 223,115 - +0.5 223,435 + PS417_01025 0.34 -0.9
Or see this region's nucleotide sequence