Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00090 and PS417_00095 are separated by 69 nucleotides PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 20.997 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.585 kb on - strand at 21.675 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.720 kb on - strand at 21.720 kb on - strand at 21.856 kb on + strand, within PS417_00100 at 21.856 kb on + strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.953 kb on + strand, within PS417_00100 at 21.973 kb on + strand, within PS417_00100 at 21.998 kb on + strand, within PS417_00100 at 21.998 kb on + strand, within PS417_00100 at 21.998 kb on + strand, within PS417_00100 at 21.999 kb on - strand, within PS417_00100 at 22.170 kb on + strand, within PS417_00100 at 22.382 kb on - strand, within PS417_00100 at 22.468 kb on + strand at 22.468 kb on + strand at 22.473 kb on - strand at 22.473 kb on - strand at 22.481 kb on - strand at 22.484 kb on + strand at 22.484 kb on + strand at 22.863 kb on - strand at 23.317 kb on + strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 20,997 - -0.4 21,565 - -2.3 21,565 - +0.4 21,565 - -0.0 21,565 - -0.9 21,585 - +3.4 21,675 - +1.7 21,678 - -2.1 21,678 - +1.4 21,678 - +1.2 21,720 - +0.4 21,720 - -0.1 21,856 + PS417_00100 0.21 +0.2 21,856 + PS417_00100 0.21 +1.7 21,857 - PS417_00100 0.21 +3.0 21,857 - PS417_00100 0.21 +0.9 21,857 - PS417_00100 0.21 -0.1 21,953 + PS417_00100 0.33 -0.4 21,973 + PS417_00100 0.36 +0.2 21,998 + PS417_00100 0.39 +0.7 21,998 + PS417_00100 0.39 +0.3 21,998 + PS417_00100 0.39 -1.4 21,999 - PS417_00100 0.39 +1.1 22,170 + PS417_00100 0.61 -2.6 22,382 - PS417_00100 0.89 +0.9 22,468 + +0.1 22,468 + +2.0 22,473 - +0.1 22,473 - -0.8 22,481 - -0.1 22,484 + -2.3 22,484 + -0.7 22,863 - -0.8 23,317 + +0.1 23,318 - +0.5 23,318 - -1.1 23,401 - +0.2
Or see this region's nucleotide sequence