Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07995 and PS417_08000 are separated by 150 nucleotides PS417_08000 and PS417_08005 are separated by 309 nucleotides
PS417_07995: PS417_07995 - LOG family protein, at 1,748,326 to 1,749,699
_07995
PS417_08000: PS417_08000 - beta-hydroxylase, at 1,749,850 to 1,750,674
_08000
PS417_08005: PS417_08005 - trans-2-enoyl-CoA reductase, at 1,750,984 to 1,752,177
_08005
Position (kb) 1749
1750
1751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1749.197 kb on + strand, within PS417_07995 at 1749.197 kb on + strand, within PS417_07995 at 1749.197 kb on + strand, within PS417_07995 at 1749.198 kb on - strand, within PS417_07995 at 1749.198 kb on - strand, within PS417_07995 at 1749.198 kb on - strand, within PS417_07995 at 1749.203 kb on + strand, within PS417_07995 at 1749.204 kb on - strand, within PS417_07995 at 1749.269 kb on - strand, within PS417_07995 at 1749.302 kb on + strand, within PS417_07995 at 1749.302 kb on + strand, within PS417_07995 at 1749.303 kb on - strand, within PS417_07995 at 1749.303 kb on - strand, within PS417_07995 at 1749.303 kb on - strand, within PS417_07995 at 1749.303 kb on - strand, within PS417_07995 at 1749.303 kb on - strand, within PS417_07995 at 1749.510 kb on - strand, within PS417_07995 at 1749.583 kb on + strand at 1749.590 kb on + strand at 1749.591 kb on - strand at 1749.591 kb on - strand at 1749.591 kb on - strand at 1749.591 kb on - strand at 1749.791 kb on - strand at 1749.795 kb on - strand at 1749.851 kb on + strand at 1750.036 kb on - strand, within PS417_08000 at 1750.059 kb on - strand, within PS417_08000 at 1750.086 kb on - strand, within PS417_08000 at 1750.086 kb on - strand, within PS417_08000 at 1750.086 kb on - strand, within PS417_08000 at 1750.121 kb on + strand, within PS417_08000 at 1750.122 kb on - strand, within PS417_08000 at 1750.268 kb on + strand, within PS417_08000 at 1750.412 kb on + strand, within PS417_08000 at 1750.412 kb on + strand, within PS417_08000 at 1750.412 kb on + strand, within PS417_08000 at 1750.413 kb on - strand, within PS417_08000 at 1750.413 kb on - strand, within PS417_08000 at 1750.413 kb on - strand, within PS417_08000 at 1750.523 kb on + strand, within PS417_08000 at 1750.524 kb on - strand, within PS417_08000 at 1750.551 kb on + strand, within PS417_08000 at 1750.552 kb on - strand, within PS417_08000 at 1750.579 kb on + strand, within PS417_08000 at 1750.579 kb on + strand, within PS417_08000 at 1750.579 kb on + strand, within PS417_08000 at 1750.580 kb on - strand, within PS417_08000 at 1750.602 kb on + strand at 1750.602 kb on + strand at 1750.602 kb on + strand at 1750.602 kb on + strand at 1750.603 kb on - strand at 1750.603 kb on - strand at 1750.603 kb on - strand at 1750.654 kb on - strand at 1750.770 kb on - strand at 1750.770 kb on - strand at 1750.834 kb on + strand at 1750.834 kb on + strand at 1750.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,749,197 + PS417_07995 0.63 -0.2 1,749,197 + PS417_07995 0.63 -0.3 1,749,197 + PS417_07995 0.63 +2.0 1,749,198 - PS417_07995 0.63 +1.1 1,749,198 - PS417_07995 0.63 +0.2 1,749,198 - PS417_07995 0.63 -1.4 1,749,203 + PS417_07995 0.64 -0.2 1,749,204 - PS417_07995 0.64 +0.8 1,749,269 - PS417_07995 0.69 -0.0 1,749,302 + PS417_07995 0.71 +0.1 1,749,302 + PS417_07995 0.71 -1.6 1,749,303 - PS417_07995 0.71 +0.1 1,749,303 - PS417_07995 0.71 +2.3 1,749,303 - PS417_07995 0.71 -1.9 1,749,303 - PS417_07995 0.71 -0.2 1,749,303 - PS417_07995 0.71 -0.5 1,749,510 - PS417_07995 0.86 -2.6 1,749,583 + +0.4 1,749,590 + -0.8 1,749,591 - -0.8 1,749,591 - +0.0 1,749,591 - -3.0 1,749,591 - -2.7 1,749,791 - +0.0 1,749,795 - +0.4 1,749,851 + -1.4 1,750,036 - PS417_08000 0.23 -2.7 1,750,059 - PS417_08000 0.25 -1.0 1,750,086 - PS417_08000 0.29 -1.3 1,750,086 - PS417_08000 0.29 +1.5 1,750,086 - PS417_08000 0.29 -3.2 1,750,121 + PS417_08000 0.33 -2.7 1,750,122 - PS417_08000 0.33 -4.0 1,750,268 + PS417_08000 0.51 -0.9 1,750,412 + PS417_08000 0.68 -0.9 1,750,412 + PS417_08000 0.68 -0.6 1,750,412 + PS417_08000 0.68 -0.2 1,750,413 - PS417_08000 0.68 -1.9 1,750,413 - PS417_08000 0.68 -2.2 1,750,413 - PS417_08000 0.68 -1.8 1,750,523 + PS417_08000 0.82 -1.6 1,750,524 - PS417_08000 0.82 +0.3 1,750,551 + PS417_08000 0.85 -0.6 1,750,552 - PS417_08000 0.85 +0.4 1,750,579 + PS417_08000 0.88 -1.6 1,750,579 + PS417_08000 0.88 -2.2 1,750,579 + PS417_08000 0.88 -0.5 1,750,580 - PS417_08000 0.88 -1.7 1,750,602 + -1.0 1,750,602 + -1.0 1,750,602 + -1.6 1,750,602 + -1.2 1,750,603 - -0.7 1,750,603 - -3.0 1,750,603 - -1.9 1,750,654 - -3.1 1,750,770 - -2.1 1,750,770 - +1.4 1,750,834 + -1.2 1,750,834 + -1.0 1,750,916 + +0.2
Or see this region's nucleotide sequence