Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06805 and PS417_06810 overlap by 1 nucleotides PS417_06810 and PS417_06815 are separated by 186 nucleotides PS417_06815 and PS417_06820 overlap by 4 nucleotides PS417_06820 and PS417_06825 are separated by 183 nucleotides
PS417_06805: PS417_06805 - glycerol acyltransferase, at 1,507,265 to 1,508,056
_06805
PS417_06810: PS417_06810 - hemolysin, at 1,508,056 to 1,508,811
_06810
PS417_06815: PS417_06815 - beta-lactamase, at 1,508,998 to 1,510,071
_06815
PS417_06820: PS417_06820 - amidase, at 1,510,068 to 1,510,382
_06820
PS417_06825: PS417_06825 - hypothetical protein, at 1,510,566 to 1,510,856
_06825
Position (kb)
1508
1509
1510
1511 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1508.013 kb on - strand at 1508.013 kb on - strand at 1508.085 kb on - strand at 1508.096 kb on - strand at 1508.096 kb on - strand at 1508.189 kb on + strand, within PS417_06810 at 1508.189 kb on + strand, within PS417_06810 at 1508.190 kb on - strand, within PS417_06810 at 1508.190 kb on - strand, within PS417_06810 at 1508.292 kb on - strand, within PS417_06810 at 1508.372 kb on + strand, within PS417_06810 at 1508.373 kb on - strand, within PS417_06810 at 1508.373 kb on - strand, within PS417_06810 at 1508.639 kb on + strand, within PS417_06810 at 1508.639 kb on + strand at 1508.640 kb on - strand, within PS417_06810 at 1508.903 kb on + strand at 1508.904 kb on - strand at 1509.211 kb on + strand, within PS417_06815 at 1509.212 kb on - strand, within PS417_06815 at 1509.431 kb on - strand, within PS417_06815 at 1509.607 kb on - strand, within PS417_06815 at 1509.613 kb on + strand, within PS417_06815 at 1509.613 kb on + strand, within PS417_06815 at 1509.613 kb on + strand, within PS417_06815 at 1509.614 kb on - strand, within PS417_06815 at 1509.614 kb on - strand, within PS417_06815 at 1509.614 kb on - strand, within PS417_06815 at 1509.615 kb on + strand, within PS417_06815 at 1509.615 kb on + strand, within PS417_06815 at 1509.615 kb on + strand, within PS417_06815 at 1509.615 kb on + strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.616 kb on - strand, within PS417_06815 at 1509.715 kb on + strand, within PS417_06815 at 1509.832 kb on - strand, within PS417_06815 at 1509.835 kb on + strand, within PS417_06815 at 1509.992 kb on + strand at 1510.197 kb on + strand at 1510.198 kb on - strand, within PS417_06820 at 1510.198 kb on - strand, within PS417_06820 at 1510.213 kb on - strand, within PS417_06820 at 1510.237 kb on - strand, within PS417_06820 at 1510.248 kb on + strand, within PS417_06820 at 1510.260 kb on + strand, within PS417_06820 at 1510.261 kb on - strand, within PS417_06820 at 1510.261 kb on - strand, within PS417_06820 at 1510.329 kb on - strand, within PS417_06820 at 1510.564 kb on + strand at 1510.564 kb on + strand at 1510.564 kb on + strand at 1510.565 kb on - strand at 1510.565 kb on - strand at 1510.567 kb on + strand at 1510.567 kb on + strand at 1510.568 kb on - strand at 1510.568 kb on - strand at 1510.640 kb on - strand, within PS417_06825 at 1510.640 kb on - strand, within PS417_06825 at 1510.826 kb on + strand, within PS417_06825 at 1510.857 kb on - strand at 1510.857 kb on - strand at 1510.857 kb on - strand at 1510.873 kb on - strand at 1510.924 kb on + strand at 1510.979 kb on - strand at 1510.989 kb on + strand at 1511.025 kb on - strand at 1511.043 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,508,013 - -0.5 1,508,013 - +1.3 1,508,085 - +1.0 1,508,096 - +1.1 1,508,096 - -1.7 1,508,189 + PS417_06810 0.18 -0.5 1,508,189 + PS417_06810 0.18 -0.7 1,508,190 - PS417_06810 0.18 -1.7 1,508,190 - PS417_06810 0.18 -0.8 1,508,292 - PS417_06810 0.31 +0.5 1,508,372 + PS417_06810 0.42 -0.0 1,508,373 - PS417_06810 0.42 +0.4 1,508,373 - PS417_06810 0.42 -1.0 1,508,639 + PS417_06810 0.77 +0.4 1,508,639 + -0.2 1,508,640 - PS417_06810 0.77 -0.3 1,508,903 + -1.5 1,508,904 - +0.2 1,509,211 + PS417_06815 0.20 +2.1 1,509,212 - PS417_06815 0.20 -1.2 1,509,431 - PS417_06815 0.40 +0.3 1,509,607 - PS417_06815 0.57 +2.3 1,509,613 + PS417_06815 0.57 +2.3 1,509,613 + PS417_06815 0.57 -1.0 1,509,613 + PS417_06815 0.57 -3.6 1,509,614 - PS417_06815 0.57 -1.3 1,509,614 - PS417_06815 0.57 +0.2 1,509,614 - PS417_06815 0.57 +0.2 1,509,615 + PS417_06815 0.57 -0.1 1,509,615 + PS417_06815 0.57 +0.8 1,509,615 + PS417_06815 0.57 -0.2 1,509,615 + PS417_06815 0.57 -0.8 1,509,616 - PS417_06815 0.58 +0.1 1,509,616 - PS417_06815 0.58 -1.6 1,509,616 - PS417_06815 0.58 -0.7 1,509,616 - PS417_06815 0.58 +0.5 1,509,616 - PS417_06815 0.58 +0.0 1,509,616 - PS417_06815 0.58 -0.7 1,509,616 - PS417_06815 0.58 -0.4 1,509,715 + PS417_06815 0.67 -1.5 1,509,832 - PS417_06815 0.78 +0.0 1,509,835 + PS417_06815 0.78 +0.6 1,509,992 + -2.0 1,510,197 + +0.3 1,510,198 - PS417_06820 0.41 +0.0 1,510,198 - PS417_06820 0.41 +0.0 1,510,213 - PS417_06820 0.46 +0.1 1,510,237 - PS417_06820 0.54 +1.4 1,510,248 + PS417_06820 0.57 -0.7 1,510,260 + PS417_06820 0.61 -0.9 1,510,261 - PS417_06820 0.61 -1.5 1,510,261 - PS417_06820 0.61 -0.2 1,510,329 - PS417_06820 0.83 -0.7 1,510,564 + +0.6 1,510,564 + +0.3 1,510,564 + -0.1 1,510,565 - +0.2 1,510,565 - -0.3 1,510,567 + +0.1 1,510,567 + +0.5 1,510,568 - -1.7 1,510,568 - -0.7 1,510,640 - PS417_06825 0.25 +1.5 1,510,640 - PS417_06825 0.25 +0.3 1,510,826 + PS417_06825 0.89 -1.8 1,510,857 - +1.3 1,510,857 - -0.7 1,510,857 - +0.7 1,510,873 - +0.9 1,510,924 + -0.3 1,510,979 - -0.1 1,510,989 + -0.9 1,511,025 - -0.2 1,511,043 + +1.2
Or see this region's nucleotide sequence