Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06710 and PS417_06715 are separated by 17 nucleotides PS417_06715 and PS417_06720 are separated by 1 nucleotides PS417_06720 and PS417_06725 are separated by 137 nucleotides
PS417_06710: PS417_06710 - beta-ketoadipyl CoA thiolase, at 1,486,185 to 1,487,387
_06710
PS417_06715: PS417_06715 - protocatechuate 3,4-dioxygenase, at 1,487,405 to 1,488,109
_06715
PS417_06720: PS417_06720 - protocatechuate 3,4-dioxygenase, at 1,488,111 to 1,488,677
_06720
PS417_06725: PS417_06725 - alpha-ketoglutarate permease, at 1,488,815 to 1,490,110
_06725
Position (kb)
1487
1488
1489 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1486.595 kb on + strand, within PS417_06710 at 1486.705 kb on - strand, within PS417_06710 at 1487.385 kb on + strand at 1487.386 kb on - strand at 1487.386 kb on - strand at 1487.386 kb on - strand at 1487.389 kb on - strand at 1487.457 kb on - strand at 1487.457 kb on - strand at 1487.471 kb on + strand at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.970 kb on - strand, within PS417_06715 at 1487.970 kb on - strand, within PS417_06715 at 1488.045 kb on - strand at 1488.150 kb on + strand at 1488.154 kb on - strand at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.405 kb on - strand, within PS417_06720 at 1488.463 kb on + strand, within PS417_06720 at 1488.464 kb on - strand, within PS417_06720 at 1488.467 kb on - strand, within PS417_06720 at 1488.467 kb on - strand, within PS417_06720 at 1488.585 kb on - strand, within PS417_06720 at 1488.619 kb on - strand, within PS417_06720 at 1488.619 kb on - strand, within PS417_06720 at 1488.738 kb on - strand at 1488.777 kb on - strand at 1488.777 kb on - strand at 1488.777 kb on - strand at 1488.787 kb on + strand at 1488.917 kb on + strand at 1488.918 kb on - strand at 1488.918 kb on - strand at 1488.918 kb on - strand at 1488.923 kb on + strand at 1488.924 kb on - strand at 1488.941 kb on + strand at 1488.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,486,595 + PS417_06710 0.34 +1.0 1,486,705 - PS417_06710 0.43 +0.5 1,487,385 + +0.4 1,487,386 - +0.9 1,487,386 - -0.2 1,487,386 - -0.1 1,487,389 - +1.4 1,487,457 - +0.3 1,487,457 - -0.0 1,487,471 + -2.1 1,487,930 + PS417_06715 0.74 -0.5 1,487,930 + PS417_06715 0.74 +0.4 1,487,930 + +0.4 1,487,930 + PS417_06715 0.74 -1.3 1,487,930 + PS417_06715 0.74 +0.3 1,487,930 + PS417_06715 0.74 -2.3 1,487,930 + PS417_06715 0.74 -2.0 1,487,930 + PS417_06715 0.74 -0.6 1,487,930 + PS417_06715 0.74 -2.1 1,487,931 - PS417_06715 0.75 -2.6 1,487,931 - PS417_06715 0.75 -0.2 1,487,931 - PS417_06715 0.75 -0.5 1,487,931 - PS417_06715 0.75 +0.0 1,487,931 - PS417_06715 0.75 +0.1 1,487,970 - PS417_06715 0.80 +0.0 1,487,970 - PS417_06715 0.80 +0.3 1,488,045 - -0.1 1,488,150 + +0.4 1,488,154 - -1.5 1,488,309 + PS417_06720 0.35 +0.9 1,488,309 + PS417_06720 0.35 +0.8 1,488,309 + PS417_06720 0.35 -0.5 1,488,309 + PS417_06720 0.35 -1.7 1,488,310 - PS417_06720 0.35 +0.6 1,488,310 - PS417_06720 0.35 +0.6 1,488,310 - PS417_06720 0.35 -0.6 1,488,310 - PS417_06720 0.35 -0.6 1,488,310 - PS417_06720 0.35 -0.5 1,488,405 - PS417_06720 0.52 +1.4 1,488,463 + PS417_06720 0.62 -0.7 1,488,464 - PS417_06720 0.62 +0.6 1,488,467 - PS417_06720 0.63 +0.1 1,488,467 - PS417_06720 0.63 -2.4 1,488,585 - PS417_06720 0.84 +0.0 1,488,619 - PS417_06720 0.90 +0.7 1,488,619 - PS417_06720 0.90 -0.0 1,488,738 - -0.1 1,488,777 - +0.8 1,488,777 - +2.1 1,488,777 - -2.7 1,488,787 + +1.1 1,488,917 + -0.5 1,488,918 - +2.4 1,488,918 - +0.2 1,488,918 - +0.6 1,488,923 + -0.5 1,488,924 - +0.4 1,488,941 + +0.6 1,488,942 - +0.4
Or see this region's nucleotide sequence