Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06460 and PS417_06465 are separated by 198 nucleotides PS417_06465 and PS417_06470 overlap by 4 nucleotides PS417_06470 and PS417_06475 are separated by 131 nucleotides PS417_06475 and PS417_06480 overlap by 4 nucleotides PS417_06480 and PS417_06485 are separated by 20 nucleotides
PS417_06460: PS417_06460 - TonB-dependent receptor, at 1,432,339 to 1,434,720
_06460
PS417_06465: PS417_06465 - transcriptional regulator, at 1,434,919 to 1,435,629
_06465
PS417_06470: PS417_06470 - histidine kinase, at 1,435,626 to 1,437,011
_06470
PS417_06475: PS417_06475 - zinc chelation protein SecC, at 1,437,143 to 1,437,343
_06475
PS417_06480: PS417_06480 - hypothetical protein, at 1,437,340 to 1,437,822
_06480
PS417_06485: PS417_06485 - hypothetical protein, at 1,437,843 to 1,438,313
_06485
Position (kb)
1435
1436
1437
1438 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1434.743 kb on - strand at 1434.772 kb on + strand at 1434.772 kb on + strand at 1434.772 kb on + strand at 1434.773 kb on - strand at 1434.773 kb on - strand at 1434.824 kb on + strand at 1434.835 kb on - strand at 1434.848 kb on - strand at 1435.219 kb on + strand, within PS417_06465 at 1435.220 kb on - strand, within PS417_06465 at 1435.370 kb on + strand, within PS417_06465 at 1435.370 kb on + strand, within PS417_06465 at 1435.883 kb on + strand, within PS417_06470 at 1435.887 kb on - strand, within PS417_06470 at 1435.943 kb on - strand, within PS417_06470 at 1435.963 kb on - strand, within PS417_06470 at 1436.166 kb on + strand, within PS417_06470 at 1436.167 kb on - strand, within PS417_06470 at 1436.167 kb on - strand, within PS417_06470 at 1436.181 kb on + strand, within PS417_06470 at 1436.182 kb on - strand, within PS417_06470 at 1436.182 kb on - strand, within PS417_06470 at 1436.200 kb on + strand, within PS417_06470 at 1436.778 kb on - strand, within PS417_06470 at 1437.027 kb on + strand at 1437.027 kb on + strand at 1437.028 kb on - strand at 1437.408 kb on - strand, within PS417_06480 at 1437.418 kb on + strand, within PS417_06480 at 1437.419 kb on - strand, within PS417_06480 at 1437.540 kb on + strand, within PS417_06480 at 1437.541 kb on - strand, within PS417_06480 at 1437.671 kb on + strand, within PS417_06480 at 1437.809 kb on + strand at 1437.809 kb on + strand at 1437.850 kb on + strand at 1437.851 kb on - strand at 1437.943 kb on + strand, within PS417_06485 at 1437.944 kb on - strand, within PS417_06485 at 1437.963 kb on + strand, within PS417_06485 at 1437.963 kb on + strand, within PS417_06485 at 1437.989 kb on + strand, within PS417_06485 at 1437.990 kb on - strand, within PS417_06485 at 1437.990 kb on - strand at 1437.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,434,743 - -1.5 1,434,772 + +0.3 1,434,772 + +0.7 1,434,772 + +1.4 1,434,773 - -3.0 1,434,773 - +0.0 1,434,824 + -1.1 1,434,835 - -1.8 1,434,848 - -0.5 1,435,219 + PS417_06465 0.42 +0.0 1,435,220 - PS417_06465 0.42 -0.3 1,435,370 + PS417_06465 0.63 -2.3 1,435,370 + PS417_06465 0.63 -2.2 1,435,883 + PS417_06470 0.19 -1.1 1,435,887 - PS417_06470 0.19 +0.8 1,435,943 - PS417_06470 0.23 +0.6 1,435,963 - PS417_06470 0.24 -0.0 1,436,166 + PS417_06470 0.39 -2.7 1,436,167 - PS417_06470 0.39 +1.0 1,436,167 - PS417_06470 0.39 -1.2 1,436,181 + PS417_06470 0.40 -0.2 1,436,182 - PS417_06470 0.40 +0.3 1,436,182 - PS417_06470 0.40 -0.4 1,436,200 + PS417_06470 0.41 -1.4 1,436,778 - PS417_06470 0.83 -0.1 1,437,027 + -1.1 1,437,027 + -0.9 1,437,028 - -0.3 1,437,408 - PS417_06480 0.14 -0.1 1,437,418 + PS417_06480 0.16 +0.1 1,437,419 - PS417_06480 0.16 +1.3 1,437,540 + PS417_06480 0.41 -0.2 1,437,541 - PS417_06480 0.42 +0.1 1,437,671 + PS417_06480 0.69 -0.7 1,437,809 + +1.1 1,437,809 + -0.9 1,437,850 + -0.4 1,437,851 - +0.2 1,437,943 + PS417_06485 0.21 +1.0 1,437,944 - PS417_06485 0.21 -1.2 1,437,963 + PS417_06485 0.25 -0.1 1,437,963 + PS417_06485 0.25 -0.3 1,437,989 + PS417_06485 0.31 -0.4 1,437,990 - PS417_06485 0.31 +0.3 1,437,990 - +1.4 1,437,990 - +0.4
Or see this region's nucleotide sequence