Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05040 and PS417_05045 are separated by 8 nucleotides PS417_05045 and PS417_05050 are separated by 247 nucleotides
PS417_05040: PS417_05040 - amino acid dehydrogenase, at 1,121,090 to 1,122,109
_05040
PS417_05045: PS417_05045 - acetamidase, at 1,122,118 to 1,123,425
_05045
PS417_05050: PS417_05050 - carboxymethylenebutenolidase, at 1,123,673 to 1,124,386
_05050
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.411 kb on + strand at 1121.412 kb on - strand, within PS417_05040 at 1121.949 kb on - strand, within PS417_05040 at 1122.110 kb on + strand at 1122.110 kb on + strand at 1122.111 kb on - strand at 1122.111 kb on - strand at 1122.128 kb on + strand at 1122.348 kb on + strand, within PS417_05045 at 1122.368 kb on - strand, within PS417_05045 at 1122.533 kb on - strand, within PS417_05045 at 1122.593 kb on + strand, within PS417_05045 at 1122.594 kb on - strand, within PS417_05045 at 1122.734 kb on + strand, within PS417_05045 at 1122.983 kb on + strand, within PS417_05045 at 1122.984 kb on - strand, within PS417_05045 at 1123.318 kb on - strand at 1123.383 kb on - strand at 1123.443 kb on + strand at 1123.471 kb on - strand at 1123.471 kb on - strand at 1123.471 kb on - strand at 1123.471 kb on - strand at 1123.501 kb on + strand at 1123.525 kb on + strand at 1123.547 kb on - strand at 1123.547 kb on - strand at 1123.556 kb on + strand at 1123.556 kb on + strand at 1123.556 kb on + strand at 1123.557 kb on - strand at 1123.557 kb on - strand at 1123.557 kb on - strand at 1123.560 kb on + strand at 1123.560 kb on + strand at 1123.561 kb on - strand at 1123.561 kb on - strand at 1123.583 kb on + strand at 1123.583 kb on + strand at 1123.584 kb on - strand at 1123.601 kb on + strand at 1123.601 kb on + strand at 1123.668 kb on + strand at 1123.668 kb on + strand at 1123.669 kb on - strand at 1123.740 kb on - strand at 1123.980 kb on - strand, within PS417_05050 at 1124.090 kb on + strand, within PS417_05050 at 1124.240 kb on + strand, within PS417_05050 at 1124.241 kb on - strand, within PS417_05050 at 1124.241 kb on - strand, within PS417_05050 at 1124.312 kb on + strand, within PS417_05050 at 1124.385 kb on - strand at 1124.395 kb on + strand at 1124.396 kb on - strand at 1124.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,121,282 + PS417_05040 0.19 +1.2 1,121,282 + PS417_05040 0.19 -3.6 1,121,282 + PS417_05040 0.19 -1.1 1,121,282 + PS417_05040 0.19 -0.3 1,121,411 + +1.0 1,121,412 - PS417_05040 0.32 -0.2 1,121,949 - PS417_05040 0.84 -1.1 1,122,110 + +0.1 1,122,110 + -0.1 1,122,111 - -0.9 1,122,111 - -0.1 1,122,128 + -0.3 1,122,348 + PS417_05045 0.18 -1.0 1,122,368 - PS417_05045 0.19 -0.5 1,122,533 - PS417_05045 0.32 -0.8 1,122,593 + PS417_05045 0.36 -0.6 1,122,594 - PS417_05045 0.36 -3.4 1,122,734 + PS417_05045 0.47 +1.5 1,122,983 + PS417_05045 0.66 +1.7 1,122,984 - PS417_05045 0.66 -0.3 1,123,318 - -0.3 1,123,383 - -2.0 1,123,443 + +0.7 1,123,471 - -1.7 1,123,471 - +0.8 1,123,471 - +1.2 1,123,471 - -0.3 1,123,501 + -2.7 1,123,525 + -1.0 1,123,547 - +0.4 1,123,547 - -1.0 1,123,556 + -0.3 1,123,556 + -1.1 1,123,556 + +1.7 1,123,557 - +0.1 1,123,557 - +0.2 1,123,557 - +0.3 1,123,560 + -3.5 1,123,560 + -0.7 1,123,561 - +1.7 1,123,561 - +1.1 1,123,583 + -0.8 1,123,583 + -1.1 1,123,584 - -1.3 1,123,601 + -0.4 1,123,601 + -0.7 1,123,668 + +0.0 1,123,668 + -0.1 1,123,669 - +2.2 1,123,740 - +0.2 1,123,980 - PS417_05050 0.43 -0.1 1,124,090 + PS417_05050 0.58 -2.2 1,124,240 + PS417_05050 0.79 +0.6 1,124,241 - PS417_05050 0.80 -1.6 1,124,241 - PS417_05050 0.80 -2.0 1,124,312 + PS417_05050 0.89 +0.3 1,124,385 - +0.7 1,124,395 + -1.2 1,124,396 - +1.3 1,124,406 - -0.7
Or see this region's nucleotide sequence