Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05020 and PS417_05025 are separated by 12 nucleotides PS417_05025 and PS417_05030 are separated by 39 nucleotides PS417_05030 and PS417_05035 overlap by 4 nucleotides PS417_05035 and PS417_05040 are separated by 187 nucleotides
PS417_05020: PS417_05020 - fumarylacetoacetase, at 1,116,755 to 1,118,041
_05020
PS417_05025: PS417_05025 - maleylacetoacetate isomerase, at 1,118,054 to 1,118,695
_05025
PS417_05030: PS417_05030 - major facilitator transporter, at 1,118,735 to 1,120,102
_05030
PS417_05035: PS417_05035 - hypothetical protein, at 1,120,099 to 1,120,902
_05035
PS417_05040: PS417_05040 - amino acid dehydrogenase, at 1,121,090 to 1,122,109
_05040
Position (kb) 1118
1119
1120
1121 Strain fitness (log2 ratio) -1
0
1
2 at 1117.754 kb on + strand, within PS417_05020 at 1118.040 kb on - strand at 1118.069 kb on + strand at 1118.070 kb on - strand at 1118.542 kb on + strand, within PS417_05025 at 1118.564 kb on + strand, within PS417_05025 at 1118.727 kb on + strand at 1118.844 kb on - strand at 1118.864 kb on + strand at 1118.865 kb on - strand at 1118.948 kb on - strand, within PS417_05030 at 1119.027 kb on - strand, within PS417_05030 at 1119.027 kb on - strand, within PS417_05030 at 1119.091 kb on + strand, within PS417_05030 at 1119.091 kb on + strand, within PS417_05030 at 1119.169 kb on + strand, within PS417_05030 at 1119.170 kb on - strand, within PS417_05030 at 1119.170 kb on - strand, within PS417_05030 at 1119.369 kb on - strand, within PS417_05030 at 1119.495 kb on - strand, within PS417_05030 at 1119.551 kb on + strand, within PS417_05030 at 1119.552 kb on - strand, within PS417_05030 at 1119.776 kb on + strand, within PS417_05030 at 1119.777 kb on - strand, within PS417_05030 at 1119.870 kb on + strand, within PS417_05030 at 1119.870 kb on + strand, within PS417_05030 at 1119.871 kb on - strand, within PS417_05030 at 1119.871 kb on - strand, within PS417_05030 at 1120.492 kb on + strand, within PS417_05035 at 1120.492 kb on + strand, within PS417_05035 at 1120.493 kb on - strand, within PS417_05035 at 1120.804 kb on + strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.982 kb on - strand at 1121.012 kb on + strand at 1121.013 kb on - strand at 1121.049 kb on + strand at 1121.050 kb on - strand at 1121.050 kb on - strand at 1121.051 kb on + strand at 1121.051 kb on + strand at 1121.052 kb on - strand at 1121.052 kb on - strand at 1121.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,117,754 + PS417_05020 0.78 +0.4 1,118,040 - -0.1 1,118,069 + -0.9 1,118,070 - +2.6 1,118,542 + PS417_05025 0.76 -1.2 1,118,564 + PS417_05025 0.79 -0.4 1,118,727 + -1.4 1,118,844 - +0.1 1,118,864 + -0.5 1,118,865 - -0.5 1,118,948 - PS417_05030 0.16 -0.3 1,119,027 - PS417_05030 0.21 -1.0 1,119,027 - PS417_05030 0.21 +0.6 1,119,091 + PS417_05030 0.26 +0.1 1,119,091 + PS417_05030 0.26 -0.7 1,119,169 + PS417_05030 0.32 -0.6 1,119,170 - PS417_05030 0.32 -0.8 1,119,170 - PS417_05030 0.32 -0.9 1,119,369 - PS417_05030 0.46 -0.9 1,119,495 - PS417_05030 0.56 +1.0 1,119,551 + PS417_05030 0.60 +0.7 1,119,552 - PS417_05030 0.60 -0.5 1,119,776 + PS417_05030 0.76 -0.4 1,119,777 - PS417_05030 0.76 -1.2 1,119,870 + PS417_05030 0.83 +0.2 1,119,870 + PS417_05030 0.83 -0.2 1,119,871 - PS417_05030 0.83 -0.2 1,119,871 - PS417_05030 0.83 +0.9 1,120,492 + PS417_05035 0.49 +0.6 1,120,492 + PS417_05035 0.49 -0.4 1,120,493 - PS417_05035 0.49 -0.6 1,120,804 + PS417_05035 0.88 -1.6 1,120,805 - PS417_05035 0.88 +1.5 1,120,805 - PS417_05035 0.88 -1.5 1,120,805 - PS417_05035 0.88 +2.1 1,120,981 + -0.4 1,120,981 + +1.6 1,120,981 + -1.3 1,120,982 - +0.2 1,121,012 + -0.3 1,121,013 - +0.7 1,121,049 + +1.4 1,121,050 - -1.4 1,121,050 - +1.4 1,121,051 + -0.0 1,121,051 + +0.7 1,121,052 - +1.0 1,121,052 - -0.6 1,121,052 - +1.1
Or see this region's nucleotide sequence