Strain Fitness in Pseudomonas simiae WCS417 around PS417_04365

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_04355 and PS417_04360 overlap by 14 nucleotidesPS417_04360 and PS417_04365 are separated by 8 nucleotidesPS417_04365 and PS417_04370 are separated by 0 nucleotides PS417_04355: PS417_04355 - organic solvent tolerance protein OstA, at 973,461 to 973,994 _04355 PS417_04360: PS417_04360 - lipopolysaccharide ABC transporter permease, at 973,981 to 974,553 _04360 PS417_04365: PS417_04365 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 974,562 to 975,086 _04365 PS417_04370: PS417_04370 - D-arabinose 5-phosphate isomerase, at 975,087 to 976,061 _04370 Position (kb) 974 975 976Strain fitness (log2 ratio) -1 0 1 2 3at 974.564 kb on - strandat 974.566 kb on - strandat 974.566 kb on - strandat 974.566 kb on - strandat 974.780 kb on - strand, within PS417_04365at 974.785 kb on - strand, within PS417_04365at 975.089 kb on - strandat 976.074 kb on - strandat 976.076 kb on - strandat 976.076 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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974,564 - -0.2
974,566 - -0.9
974,566 - -0.7
974,566 - -0.4
974,780 - PS417_04365 0.42 +3.2
974,785 - PS417_04365 0.42 +3.0
975,089 - +0.7
976,074 - -1.0
976,076 - -0.9
976,076 - +1.7

Or see this region's nucleotide sequence