Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03980 and PS417_03985 are separated by 99 nucleotides PS417_03985 and PS417_03990 are separated by 136 nucleotides
PS417_03980: PS417_03980 - alkaline phosphatase, at 891,073 to 892,614
_03980
PS417_03985: PS417_03985 - flavodoxin, at 892,714 to 895,239
_03985
PS417_03990: PS417_03990 - ligand-gated channel protein, at 895,376 to 897,661
_03990
Position (kb) 892
893
894
895
896 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 891.733 kb on + strand, within PS417_03980 at 891.914 kb on - strand, within PS417_03980 at 891.914 kb on - strand, within PS417_03980 at 891.973 kb on + strand, within PS417_03980 at 891.974 kb on - strand, within PS417_03980 at 892.143 kb on + strand, within PS417_03980 at 892.158 kb on - strand at 892.172 kb on + strand, within PS417_03980 at 892.172 kb on + strand, within PS417_03980 at 892.296 kb on - strand, within PS417_03980 at 892.318 kb on + strand, within PS417_03980 at 892.336 kb on + strand, within PS417_03980 at 892.336 kb on + strand, within PS417_03980 at 892.529 kb on + strand at 892.530 kb on - strand at 892.530 kb on - strand at 892.530 kb on - strand at 892.530 kb on - strand at 892.530 kb on - strand at 892.663 kb on + strand at 892.777 kb on + strand at 892.777 kb on + strand at 892.778 kb on - strand at 892.778 kb on - strand at 892.778 kb on - strand at 892.835 kb on + strand at 892.835 kb on + strand at 892.836 kb on - strand at 892.836 kb on - strand at 892.863 kb on + strand at 892.864 kb on - strand at 892.864 kb on - strand at 893.301 kb on - strand, within PS417_03985 at 893.564 kb on - strand, within PS417_03985 at 893.810 kb on + strand, within PS417_03985 at 893.811 kb on - strand, within PS417_03985 at 893.855 kb on - strand, within PS417_03985 at 893.855 kb on - strand, within PS417_03985 at 894.251 kb on + strand, within PS417_03985 at 894.401 kb on + strand, within PS417_03985 at 894.404 kb on + strand, within PS417_03985 at 894.405 kb on - strand, within PS417_03985 at 894.449 kb on + strand, within PS417_03985 at 894.480 kb on - strand, within PS417_03985 at 894.480 kb on - strand, within PS417_03985 at 894.480 kb on - strand, within PS417_03985 at 894.483 kb on + strand, within PS417_03985 at 894.503 kb on + strand, within PS417_03985 at 894.503 kb on + strand at 894.504 kb on - strand, within PS417_03985 at 894.602 kb on + strand, within PS417_03985 at 894.602 kb on + strand, within PS417_03985 at 894.693 kb on + strand, within PS417_03985 at 894.693 kb on + strand, within PS417_03985 at 894.693 kb on + strand, within PS417_03985 at 894.694 kb on - strand, within PS417_03985 at 894.908 kb on + strand, within PS417_03985 at 894.908 kb on + strand, within PS417_03985 at 894.909 kb on - strand, within PS417_03985 at 894.909 kb on - strand, within PS417_03985 at 894.920 kb on + strand, within PS417_03985 at 895.106 kb on - strand at 895.265 kb on - strand at 895.268 kb on + strand at 895.268 kb on + strand at 895.269 kb on - strand at 895.269 kb on - strand at 895.367 kb on + strand at 895.367 kb on + strand at 895.367 kb on + strand at 895.377 kb on + strand at 895.378 kb on - strand at 895.408 kb on + strand at 895.458 kb on + strand at 895.458 kb on + strand at 895.458 kb on + strand at 895.458 kb on + strand at 895.518 kb on + strand at 895.545 kb on + strand at 895.546 kb on - strand at 895.554 kb on - strand at 895.554 kb on - strand at 895.557 kb on + strand at 895.557 kb on + strand at 895.558 kb on - strand at 895.638 kb on + strand, within PS417_03990 at 895.638 kb on + strand, within PS417_03990 at 895.761 kb on + strand, within PS417_03990 at 895.762 kb on - strand, within PS417_03990 at 895.854 kb on + strand, within PS417_03990 at 895.854 kb on + strand, within PS417_03990 at 896.074 kb on - strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.082 kb on + strand, within PS417_03990 at 896.083 kb on - strand, within PS417_03990 at 896.083 kb on - strand, within PS417_03990 at 896.083 kb on - strand, within PS417_03990 at 896.083 kb on - strand, within PS417_03990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 891,733 + PS417_03980 0.43 +0.6 891,914 - PS417_03980 0.55 +1.3 891,914 - PS417_03980 0.55 -0.5 891,973 + PS417_03980 0.58 -0.3 891,974 - PS417_03980 0.58 +0.4 892,143 + PS417_03980 0.69 -0.9 892,158 - +2.7 892,172 + PS417_03980 0.71 -1.1 892,172 + PS417_03980 0.71 -0.7 892,296 - PS417_03980 0.79 -0.7 892,318 + PS417_03980 0.81 +1.1 892,336 + PS417_03980 0.82 -3.0 892,336 + PS417_03980 0.82 +1.7 892,529 + +0.2 892,530 - -0.6 892,530 - +0.9 892,530 - -0.6 892,530 - +1.0 892,530 - -0.3 892,663 + +3.2 892,777 + +0.5 892,777 + +0.7 892,778 - -0.5 892,778 - -0.2 892,778 - -0.9 892,835 + +0.1 892,835 + +0.1 892,836 - -0.6 892,836 - +1.1 892,863 + -0.5 892,864 - -1.0 892,864 - -1.9 893,301 - PS417_03985 0.23 +0.2 893,564 - PS417_03985 0.34 +0.2 893,810 + PS417_03985 0.43 -1.1 893,811 - PS417_03985 0.43 +0.2 893,855 - PS417_03985 0.45 -1.7 893,855 - PS417_03985 0.45 +0.6 894,251 + PS417_03985 0.61 -0.2 894,401 + PS417_03985 0.67 +0.0 894,404 + PS417_03985 0.67 -0.4 894,405 - PS417_03985 0.67 +1.8 894,449 + PS417_03985 0.69 -0.9 894,480 - PS417_03985 0.70 +0.1 894,480 - PS417_03985 0.70 -1.4 894,480 - PS417_03985 0.70 +1.4 894,483 + PS417_03985 0.70 -1.3 894,503 + PS417_03985 0.71 -0.6 894,503 + +0.7 894,504 - PS417_03985 0.71 +1.3 894,602 + PS417_03985 0.75 +0.1 894,602 + PS417_03985 0.75 -0.7 894,693 + PS417_03985 0.78 -1.5 894,693 + PS417_03985 0.78 -1.6 894,693 + PS417_03985 0.78 -0.6 894,694 - PS417_03985 0.78 -0.0 894,908 + PS417_03985 0.87 -2.0 894,908 + PS417_03985 0.87 -1.1 894,909 - PS417_03985 0.87 +0.1 894,909 - PS417_03985 0.87 +0.5 894,920 + PS417_03985 0.87 -0.3 895,106 - -0.6 895,265 - -0.3 895,268 + -0.3 895,268 + +0.2 895,269 - -0.9 895,269 - -2.5 895,367 + -1.6 895,367 + +0.1 895,367 + +0.1 895,377 + +1.7 895,378 - +0.7 895,408 + -1.6 895,458 + -1.9 895,458 + +0.2 895,458 + -0.0 895,458 + -0.9 895,518 + +0.6 895,545 + -0.2 895,546 - +0.8 895,554 - -1.2 895,554 - +0.1 895,557 + -0.3 895,557 + +0.8 895,558 - -0.2 895,638 + PS417_03990 0.11 -0.8 895,638 + PS417_03990 0.11 +1.1 895,761 + PS417_03990 0.17 -0.3 895,762 - PS417_03990 0.17 -0.6 895,854 + PS417_03990 0.21 -0.7 895,854 + PS417_03990 0.21 -0.6 896,074 - PS417_03990 0.31 +0.5 896,082 + PS417_03990 0.31 -0.8 896,082 + PS417_03990 0.31 -1.7 896,082 + PS417_03990 0.31 +0.1 896,082 + PS417_03990 0.31 -0.6 896,082 + PS417_03990 0.31 +0.7 896,082 + PS417_03990 0.31 +1.1 896,083 - PS417_03990 0.31 +0.5 896,083 - PS417_03990 0.31 -0.5 896,083 - PS417_03990 0.31 +0.3 896,083 - PS417_03990 0.31 +0.8
Or see this region's nucleotide sequence