Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03400 and PS417_03405 overlap by 11 nucleotides PS417_03405 and PS417_03410 are separated by 25 nucleotides PS417_03410 and PS417_03415 are separated by 54 nucleotides
PS417_03400: PS417_03400 - D-alanine--D-alanine ligase, at 779,984 to 781,078
_03400
PS417_03405: PS417_03405 - D-alanyl-alanine synthetase, at 781,068 to 781,793
_03405
PS417_03410: PS417_03410 - gamma-glutamyltransferase, at 781,819 to 783,630
_03410
PS417_03415: PS417_03415 - isomerase, at 783,685 to 784,464
_03415
Position (kb) 781
782
783
784 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 780.966 kb on - strand, within PS417_03400 at 780.999 kb on - strand at 781.096 kb on + strand at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.495 kb on - strand at 781.620 kb on - strand, within PS417_03405 at 781.621 kb on + strand, within PS417_03405 at 781.621 kb on + strand at 781.621 kb on + strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.623 kb on + strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.778 kb on + strand at 781.817 kb on - strand at 782.099 kb on + strand, within PS417_03410 at 782.100 kb on - strand, within PS417_03410 at 782.415 kb on - strand, within PS417_03410 at 782.817 kb on - strand, within PS417_03410 at 782.817 kb on - strand, within PS417_03410 at 782.904 kb on - strand, within PS417_03410 at 783.011 kb on + strand, within PS417_03410 at 783.480 kb on + strand at 783.710 kb on + strand at 783.711 kb on - strand at 783.846 kb on + strand, within PS417_03415 at 783.847 kb on - strand, within PS417_03415 at 784.028 kb on + strand, within PS417_03415 at 784.028 kb on + strand, within PS417_03415 at 784.028 kb on + strand, within PS417_03415 at 784.320 kb on - strand, within PS417_03415 at 784.452 kb on + strand at 784.481 kb on - strand at 784.481 kb on - strand at 784.499 kb on + strand at 784.500 kb on - strand at 784.500 kb on - strand at 784.608 kb on + strand at 784.608 kb on + strand at 784.609 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 780,966 - PS417_03400 0.90 -1.7 780,999 - -1.9 781,096 + +0.3 781,168 + PS417_03405 0.14 -1.0 781,168 + PS417_03405 0.14 +0.8 781,168 + PS417_03405 0.14 -0.3 781,405 + PS417_03405 0.46 -0.1 781,405 + PS417_03405 0.46 +0.2 781,406 - PS417_03405 0.47 -0.2 781,406 - PS417_03405 0.47 +1.5 781,494 + PS417_03405 0.59 +0.6 781,494 + PS417_03405 0.59 +0.5 781,495 - +0.2 781,620 - PS417_03405 0.76 +0.2 781,621 + PS417_03405 0.76 +0.3 781,621 + +1.1 781,621 + PS417_03405 0.76 +0.2 781,622 - PS417_03405 0.76 +0.1 781,622 - PS417_03405 0.76 +0.1 781,622 - PS417_03405 0.76 +1.2 781,622 - PS417_03405 0.76 -1.1 781,622 - PS417_03405 0.76 +0.9 781,622 - PS417_03405 0.76 +0.0 781,623 + PS417_03405 0.76 -0.6 781,624 - PS417_03405 0.77 +1.6 781,624 - PS417_03405 0.77 -0.5 781,624 - PS417_03405 0.77 +0.4 781,778 + -0.0 781,817 - +0.4 782,099 + PS417_03410 0.15 -0.6 782,100 - PS417_03410 0.16 -0.7 782,415 - PS417_03410 0.33 -0.4 782,817 - PS417_03410 0.55 +0.3 782,817 - PS417_03410 0.55 -4.3 782,904 - PS417_03410 0.60 -0.1 783,011 + PS417_03410 0.66 -1.4 783,480 + -0.4 783,710 + -0.7 783,711 - -1.6 783,846 + PS417_03415 0.21 -0.9 783,847 - PS417_03415 0.21 -0.2 784,028 + PS417_03415 0.44 +0.8 784,028 + PS417_03415 0.44 +0.5 784,028 + PS417_03415 0.44 -0.4 784,320 - PS417_03415 0.81 +0.4 784,452 + -0.4 784,481 - +0.8 784,481 - -0.6 784,499 + -1.1 784,500 - +0.8 784,500 - +0.6 784,608 + -0.3 784,608 + -0.5 784,609 - -0.5
Or see this region's nucleotide sequence