Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03290 and PS417_03295 are separated by 148 nucleotides PS417_03295 and PS417_03300 overlap by 8 nucleotides PS417_03300 and PS417_03305 are separated by 120 nucleotides
PS417_03290: PS417_03290 - hypothetical protein, at 754,917 to 756,194
_03290
PS417_03295: PS417_03295 - energy transducer TonB, at 756,343 to 757,128
_03295
PS417_03300: PS417_03300 - alanine acetyltransferase, at 757,121 to 757,573
_03300
PS417_03305: PS417_03305 - HPr, at 757,694 to 758,098
_03305
Position (kb) 756
757
758 Strain fitness (log2 ratio) -2
-1
0
1
2 at 755.860 kb on + strand, within PS417_03290 at 755.861 kb on - strand, within PS417_03290 at 755.938 kb on + strand, within PS417_03290 at 756.145 kb on - strand at 756.145 kb on - strand at 756.145 kb on - strand at 756.219 kb on + strand at 756.219 kb on + strand at 756.219 kb on + strand at 756.220 kb on - strand at 756.234 kb on + strand at 756.235 kb on - strand at 756.323 kb on + strand at 757.134 kb on + strand at 757.245 kb on - strand, within PS417_03300 at 757.338 kb on + strand, within PS417_03300 at 757.469 kb on + strand, within PS417_03300 at 757.470 kb on - strand, within PS417_03300 at 757.512 kb on - strand, within PS417_03300 at 757.512 kb on - strand, within PS417_03300 at 757.515 kb on - strand, within PS417_03300 at 758.123 kb on + strand at 758.125 kb on + strand at 758.125 kb on + strand at 758.125 kb on + strand at 758.126 kb on - strand at 758.126 kb on - strand at 758.126 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 755,860 + PS417_03290 0.74 -0.5 755,861 - PS417_03290 0.74 -0.6 755,938 + PS417_03290 0.80 -0.8 756,145 - +0.4 756,145 - -2.2 756,145 - +2.0 756,219 + -1.9 756,219 + -1.2 756,219 + -0.2 756,220 - -0.4 756,234 + -1.9 756,235 - -0.4 756,323 + -1.2 757,134 + -0.7 757,245 - PS417_03300 0.27 +0.3 757,338 + PS417_03300 0.48 -0.2 757,469 + PS417_03300 0.77 +1.8 757,470 - PS417_03300 0.77 -0.9 757,512 - PS417_03300 0.86 -1.1 757,512 - PS417_03300 0.86 -2.6 757,515 - PS417_03300 0.87 -0.6 758,123 + -0.2 758,125 + +0.2 758,125 + +0.1 758,125 + -0.1 758,126 - +0.3 758,126 - +0.8 758,126 - -2.0
Or see this region's nucleotide sequence