Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03035 and PS417_03040 are separated by 24 nucleotides PS417_03040 and PS417_03045 are separated by 45 nucleotides PS417_03045 and PS417_03050 are separated by 155 nucleotides
PS417_03035: PS417_03035 - SAM-dependent methyltransferase, at 698,644 to 699,393
_03035
PS417_03040: PS417_03040 - hypothetical protein, at 699,418 to 700,020
_03040
PS417_03045: PS417_03045 - hypothetical protein, at 700,066 to 700,623
_03045
PS417_03050: PS417_03050 - hypothetical protein, at 700,779 to 702,725
_03050
Position (kb) 700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 699.168 kb on + strand, within PS417_03035 at 699.169 kb on - strand, within PS417_03035 at 699.265 kb on + strand, within PS417_03035 at 699.265 kb on + strand, within PS417_03035 at 699.265 kb on + strand, within PS417_03035 at 699.265 kb on + strand, within PS417_03035 at 699.265 kb on + strand, within PS417_03035 at 699.266 kb on - strand, within PS417_03035 at 699.266 kb on - strand, within PS417_03035 at 699.266 kb on - strand, within PS417_03035 at 699.266 kb on - strand, within PS417_03035 at 699.267 kb on + strand, within PS417_03035 at 699.267 kb on + strand, within PS417_03035 at 699.267 kb on + strand, within PS417_03035 at 699.267 kb on + strand, within PS417_03035 at 699.268 kb on - strand, within PS417_03035 at 699.268 kb on - strand, within PS417_03035 at 699.268 kb on - strand, within PS417_03035 at 699.268 kb on - strand, within PS417_03035 at 699.365 kb on - strand at 699.368 kb on + strand at 699.392 kb on - strand at 699.571 kb on + strand, within PS417_03040 at 699.572 kb on - strand, within PS417_03040 at 699.585 kb on + strand, within PS417_03040 at 699.586 kb on - strand, within PS417_03040 at 699.826 kb on + strand, within PS417_03040 at 699.826 kb on + strand, within PS417_03040 at 699.826 kb on + strand, within PS417_03040 at 699.827 kb on - strand, within PS417_03040 at 699.853 kb on + strand, within PS417_03040 at 699.853 kb on + strand, within PS417_03040 at 699.853 kb on + strand, within PS417_03040 at 699.854 kb on - strand, within PS417_03040 at 699.854 kb on - strand, within PS417_03040 at 700.084 kb on - strand at 700.381 kb on + strand, within PS417_03045 at 700.432 kb on + strand, within PS417_03045 at 700.689 kb on + strand at 700.690 kb on - strand at 700.735 kb on - strand at 700.735 kb on - strand at 700.735 kb on - strand at 700.750 kb on + strand at 700.750 kb on + strand at 700.751 kb on - strand at 700.751 kb on - strand at 700.859 kb on + strand at 700.860 kb on - strand at 700.917 kb on - strand at 700.989 kb on + strand, within PS417_03050 at 701.418 kb on + strand, within PS417_03050 at 701.573 kb on + strand, within PS417_03050 at 701.609 kb on - strand, within PS417_03050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 699,168 + PS417_03035 0.70 -1.3 699,169 - PS417_03035 0.70 +0.8 699,265 + PS417_03035 0.83 -0.9 699,265 + PS417_03035 0.83 +0.8 699,265 + PS417_03035 0.83 +0.8 699,265 + PS417_03035 0.83 -3.2 699,265 + PS417_03035 0.83 -0.4 699,266 - PS417_03035 0.83 -1.0 699,266 - PS417_03035 0.83 +0.4 699,266 - PS417_03035 0.83 +0.6 699,266 - PS417_03035 0.83 +0.9 699,267 + PS417_03035 0.83 -0.2 699,267 + PS417_03035 0.83 -0.8 699,267 + PS417_03035 0.83 +1.4 699,267 + PS417_03035 0.83 +0.1 699,268 - PS417_03035 0.83 -2.2 699,268 - PS417_03035 0.83 -1.2 699,268 - PS417_03035 0.83 -0.8 699,268 - PS417_03035 0.83 +0.4 699,365 - +1.0 699,368 + -0.5 699,392 - -0.8 699,571 + PS417_03040 0.25 +0.7 699,572 - PS417_03040 0.26 -1.2 699,585 + PS417_03040 0.28 +0.1 699,586 - PS417_03040 0.28 -0.1 699,826 + PS417_03040 0.68 -0.4 699,826 + PS417_03040 0.68 -2.3 699,826 + PS417_03040 0.68 -1.2 699,827 - PS417_03040 0.68 -0.6 699,853 + PS417_03040 0.72 +0.2 699,853 + PS417_03040 0.72 -0.7 699,853 + PS417_03040 0.72 -1.1 699,854 - PS417_03040 0.72 +0.5 699,854 - PS417_03040 0.72 -1.0 700,084 - -1.1 700,381 + PS417_03045 0.56 +0.3 700,432 + PS417_03045 0.66 +0.9 700,689 + -1.8 700,690 - +0.8 700,735 - +0.9 700,735 - +0.1 700,735 - -0.6 700,750 + +0.3 700,750 + +1.8 700,751 - -0.2 700,751 - +0.2 700,859 + +0.7 700,860 - +0.0 700,917 - -0.2 700,989 + PS417_03050 0.11 +0.1 701,418 + PS417_03050 0.33 -1.5 701,573 + PS417_03050 0.41 +0.1 701,609 - PS417_03050 0.43 -0.1
Or see this region's nucleotide sequence