Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27830 and PS417_27835 are separated by 24 nucleotides PS417_27835 and PS417_27840 are separated by 56 nucleotides PS417_27840 and PS417_27845 are separated by 124 nucleotides
PS417_27830: PS417_27830 - (p)ppGpp synthetase, at 5,999,083 to 6,001,188
_27830
PS417_27835: PS417_27835 - endoribonuclease, at 6,001,213 to 6,001,593
_27835
PS417_27840: PS417_27840 - hypothetical protein, at 6,001,650 to 6,002,393
_27840
PS417_27845: PS417_27845 - oxidoreductase, at 6,002,518 to 6,003,369
_27845
Position (kb)
6001
6002
6003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6001.195 kb on + strand at 6001.198 kb on + strand at 6001.198 kb on + strand at 6001.199 kb on - strand at 6001.199 kb on - strand at 6001.228 kb on - strand at 6001.265 kb on - strand, within PS417_27835 at 6001.265 kb on - strand, within PS417_27835 at 6001.297 kb on + strand, within PS417_27835 at 6001.298 kb on - strand, within PS417_27835 at 6001.367 kb on + strand, within PS417_27835 at 6001.496 kb on - strand, within PS417_27835 at 6001.496 kb on - strand at 6001.496 kb on - strand, within PS417_27835 at 6001.666 kb on + strand at 6001.667 kb on - strand at 6001.836 kb on - strand, within PS417_27840 at 6001.858 kb on - strand, within PS417_27840 at 6001.860 kb on + strand, within PS417_27840 at 6001.861 kb on - strand, within PS417_27840 at 6002.076 kb on + strand, within PS417_27840 at 6002.077 kb on - strand, within PS417_27840 at 6002.085 kb on + strand, within PS417_27840 at 6002.086 kb on - strand, within PS417_27840 at 6002.086 kb on - strand, within PS417_27840 at 6002.137 kb on + strand, within PS417_27840 at 6002.138 kb on - strand, within PS417_27840 at 6002.138 kb on - strand, within PS417_27840 at 6002.391 kb on + strand at 6002.391 kb on + strand at 6002.391 kb on + strand at 6002.392 kb on - strand at 6002.392 kb on - strand at 6002.589 kb on + strand at 6002.724 kb on - strand, within PS417_27845 at 6002.724 kb on - strand, within PS417_27845 at 6002.732 kb on + strand, within PS417_27845 at 6002.733 kb on - strand, within PS417_27845 at 6002.752 kb on + strand, within PS417_27845 at 6002.813 kb on + strand, within PS417_27845 at 6002.814 kb on - strand, within PS417_27845 at 6002.815 kb on + strand, within PS417_27845 at 6002.815 kb on + strand, within PS417_27845 at 6002.815 kb on + strand, within PS417_27845 at 6002.815 kb on + strand, within PS417_27845 at 6002.816 kb on - strand, within PS417_27845 at 6002.816 kb on - strand, within PS417_27845 at 6002.816 kb on - strand, within PS417_27845 at 6002.816 kb on - strand, within PS417_27845 at 6002.885 kb on + strand, within PS417_27845 at 6002.886 kb on - strand, within PS417_27845 at 6002.886 kb on - strand, within PS417_27845 at 6003.044 kb on - strand, within PS417_27845 at 6003.112 kb on + strand, within PS417_27845 at 6003.112 kb on + strand, within PS417_27845 at 6003.113 kb on - strand, within PS417_27845 at 6003.113 kb on - strand, within PS417_27845 at 6003.116 kb on + strand, within PS417_27845 at 6003.325 kb on - strand at 6003.328 kb on + strand at 6003.328 kb on + strand at 6003.328 kb on + strand at 6003.328 kb on + strand at 6003.328 kb on + strand at 6003.328 kb on + strand at 6003.329 kb on - strand at 6003.329 kb on - strand at 6003.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 6,001,195 + -0.9 6,001,198 + -1.4 6,001,198 + -0.6 6,001,199 - +0.2 6,001,199 - +0.1 6,001,228 - +0.2 6,001,265 - PS417_27835 0.14 +0.2 6,001,265 - PS417_27835 0.14 -0.0 6,001,297 + PS417_27835 0.22 +0.8 6,001,298 - PS417_27835 0.22 -0.1 6,001,367 + PS417_27835 0.40 -1.3 6,001,496 - PS417_27835 0.74 -0.1 6,001,496 - -0.5 6,001,496 - PS417_27835 0.74 -0.6 6,001,666 + +0.6 6,001,667 - -0.5 6,001,836 - PS417_27840 0.25 +0.8 6,001,858 - PS417_27840 0.28 -1.4 6,001,860 + PS417_27840 0.28 -0.1 6,001,861 - PS417_27840 0.28 -0.2 6,002,076 + PS417_27840 0.57 -1.2 6,002,077 - PS417_27840 0.57 +0.8 6,002,085 + PS417_27840 0.58 -0.1 6,002,086 - PS417_27840 0.59 +0.6 6,002,086 - PS417_27840 0.59 -1.0 6,002,137 + PS417_27840 0.65 -1.6 6,002,138 - PS417_27840 0.66 +0.5 6,002,138 - PS417_27840 0.66 -1.6 6,002,391 + -1.0 6,002,391 + -0.2 6,002,391 + -1.4 6,002,392 - -0.9 6,002,392 - +0.9 6,002,589 + +2.1 6,002,724 - PS417_27845 0.24 -0.5 6,002,724 - PS417_27845 0.24 +1.1 6,002,732 + PS417_27845 0.25 -0.2 6,002,733 - PS417_27845 0.25 -1.2 6,002,752 + PS417_27845 0.27 +0.3 6,002,813 + PS417_27845 0.35 -1.4 6,002,814 - PS417_27845 0.35 -1.5 6,002,815 + PS417_27845 0.35 -0.3 6,002,815 + PS417_27845 0.35 +0.7 6,002,815 + PS417_27845 0.35 +2.5 6,002,815 + PS417_27845 0.35 -1.0 6,002,816 - PS417_27845 0.35 -1.2 6,002,816 - PS417_27845 0.35 -1.4 6,002,816 - PS417_27845 0.35 -1.9 6,002,816 - PS417_27845 0.35 -0.2 6,002,885 + PS417_27845 0.43 +0.7 6,002,886 - PS417_27845 0.43 -0.1 6,002,886 - PS417_27845 0.43 +1.3 6,003,044 - PS417_27845 0.62 +0.6 6,003,112 + PS417_27845 0.70 -0.5 6,003,112 + PS417_27845 0.70 -0.4 6,003,113 - PS417_27845 0.70 +1.7 6,003,113 - PS417_27845 0.70 +0.5 6,003,116 + PS417_27845 0.70 -0.5 6,003,325 - -1.4 6,003,328 + -0.6 6,003,328 + +0.1 6,003,328 + +1.3 6,003,328 + +1.2 6,003,328 + +0.3 6,003,328 + +0.7 6,003,329 - +0.5 6,003,329 - +0.8 6,003,329 - -1.0
Or see this region's nucleotide sequence