Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27115 and PS417_27120 are separated by 66 nucleotides PS417_27120 and PS417_27125 are separated by 153 nucleotides
PS417_27115: PS417_27115 - putrescine ABC transporter, ATPase component (from data) , at 5,852,821 to 5,853,963
_27115
PS417_27120: PS417_27120 - ABC transporter substrate-binding protein, at 5,854,030 to 5,855,124
_27120
PS417_27125: PS417_27125 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,855,278 to 5,856,372
_27125
Position (kb)
5854
5855
5856 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5853.116 kb on + strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.117 kb on - strand, within PS417_27115 at 5853.242 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.639 kb on + strand, within PS417_27115 at 5853.726 kb on - strand, within PS417_27115 at 5853.939 kb on + strand at 5853.941 kb on + strand at 5853.966 kb on - strand at 5854.031 kb on + strand at 5854.032 kb on - strand at 5854.032 kb on - strand at 5854.032 kb on - strand at 5854.211 kb on + strand, within PS417_27120 at 5854.212 kb on - strand, within PS417_27120 at 5854.250 kb on + strand, within PS417_27120 at 5854.250 kb on + strand, within PS417_27120 at 5854.347 kb on - strand, within PS417_27120 at 5854.457 kb on + strand, within PS417_27120 at 5854.457 kb on + strand, within PS417_27120 at 5854.475 kb on + strand, within PS417_27120 at 5854.476 kb on - strand, within PS417_27120 at 5854.476 kb on - strand, within PS417_27120 at 5854.539 kb on + strand, within PS417_27120 at 5854.540 kb on - strand, within PS417_27120 at 5854.562 kb on + strand, within PS417_27120 at 5854.562 kb on + strand, within PS417_27120 at 5854.563 kb on - strand, within PS417_27120 at 5854.563 kb on - strand, within PS417_27120 at 5854.739 kb on + strand, within PS417_27120 at 5854.752 kb on - strand, within PS417_27120 at 5854.961 kb on + strand, within PS417_27120 at 5855.034 kb on - strand at 5855.037 kb on + strand at 5855.037 kb on + strand at 5855.037 kb on + strand at 5855.038 kb on - strand at 5855.038 kb on - strand at 5855.038 kb on - strand at 5855.038 kb on - strand at 5855.138 kb on + strand at 5855.139 kb on - strand at 5855.142 kb on + strand at 5855.142 kb on + strand at 5855.189 kb on + strand at 5855.229 kb on - strand at 5855.355 kb on - strand at 5855.384 kb on + strand at 5855.384 kb on + strand at 5855.498 kb on + strand, within PS417_27125 at 5855.498 kb on + strand, within PS417_27125 at 5855.498 kb on + strand, within PS417_27125 at 5855.499 kb on - strand, within PS417_27125 at 5855.499 kb on - strand, within PS417_27125 at 5855.499 kb on - strand, within PS417_27125 at 5855.513 kb on + strand, within PS417_27125 at 5855.514 kb on - strand, within PS417_27125 at 5855.514 kb on - strand, within PS417_27125 at 5855.680 kb on - strand, within PS417_27125 at 5855.680 kb on - strand, within PS417_27125 at 5855.705 kb on + strand, within PS417_27125 at 5855.705 kb on + strand, within PS417_27125 at 5855.705 kb on + strand, within PS417_27125 at 5855.706 kb on - strand, within PS417_27125 at 5855.706 kb on - strand, within PS417_27125 at 5855.811 kb on - strand, within PS417_27125 at 5855.811 kb on - strand, within PS417_27125 at 5855.811 kb on - strand, within PS417_27125 at 5855.988 kb on - strand, within PS417_27125 at 5855.988 kb on - strand, within PS417_27125 at 5855.988 kb on - strand, within PS417_27125 at 5855.988 kb on - strand at 5856.060 kb on - strand, within PS417_27125 at 5856.092 kb on + strand, within PS417_27125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,853,116 + PS417_27115 0.26 -0.0 5,853,117 - PS417_27115 0.26 -2.5 5,853,117 - PS417_27115 0.26 +0.1 5,853,117 - PS417_27115 0.26 -2.2 5,853,117 - PS417_27115 0.26 -0.2 5,853,242 + PS417_27115 0.37 -0.2 5,853,639 + PS417_27115 0.72 -0.5 5,853,639 + PS417_27115 0.72 -0.6 5,853,639 + PS417_27115 0.72 -2.0 5,853,639 + PS417_27115 0.72 -0.0 5,853,726 - PS417_27115 0.79 -2.0 5,853,939 + +0.0 5,853,941 + -1.7 5,853,966 - +0.9 5,854,031 + -0.6 5,854,032 - +0.7 5,854,032 - +0.7 5,854,032 - +1.3 5,854,211 + PS417_27120 0.17 -2.0 5,854,212 - PS417_27120 0.17 -2.3 5,854,250 + PS417_27120 0.20 -0.9 5,854,250 + PS417_27120 0.20 -0.6 5,854,347 - PS417_27120 0.29 +0.2 5,854,457 + PS417_27120 0.39 -0.3 5,854,457 + PS417_27120 0.39 -2.7 5,854,475 + PS417_27120 0.41 +0.9 5,854,476 - PS417_27120 0.41 +0.1 5,854,476 - PS417_27120 0.41 -0.4 5,854,539 + PS417_27120 0.46 -1.8 5,854,540 - PS417_27120 0.47 -1.7 5,854,562 + PS417_27120 0.49 -1.2 5,854,562 + PS417_27120 0.49 -0.6 5,854,563 - PS417_27120 0.49 +0.6 5,854,563 - PS417_27120 0.49 +0.8 5,854,739 + PS417_27120 0.65 -2.4 5,854,752 - PS417_27120 0.66 +1.2 5,854,961 + PS417_27120 0.85 -1.8 5,855,034 - +0.2 5,855,037 + +1.2 5,855,037 + -2.9 5,855,037 + -1.4 5,855,038 - -0.7 5,855,038 - +1.1 5,855,038 - +0.8 5,855,038 - -1.2 5,855,138 + -0.8 5,855,139 - -0.9 5,855,142 + +0.9 5,855,142 + -0.8 5,855,189 + -0.9 5,855,229 - -1.4 5,855,355 - -2.5 5,855,384 + -1.3 5,855,384 + -1.6 5,855,498 + PS417_27125 0.20 -1.0 5,855,498 + PS417_27125 0.20 +0.2 5,855,498 + PS417_27125 0.20 -0.4 5,855,499 - PS417_27125 0.20 -3.0 5,855,499 - PS417_27125 0.20 -1.4 5,855,499 - PS417_27125 0.20 -0.8 5,855,513 + PS417_27125 0.21 +0.8 5,855,514 - PS417_27125 0.22 -2.8 5,855,514 - PS417_27125 0.22 -1.0 5,855,680 - PS417_27125 0.37 -0.0 5,855,680 - PS417_27125 0.37 +0.5 5,855,705 + PS417_27125 0.39 -1.1 5,855,705 + PS417_27125 0.39 +0.8 5,855,705 + PS417_27125 0.39 -1.8 5,855,706 - PS417_27125 0.39 -1.3 5,855,706 - PS417_27125 0.39 -1.5 5,855,811 - PS417_27125 0.49 -0.3 5,855,811 - PS417_27125 0.49 -2.2 5,855,811 - PS417_27125 0.49 -1.8 5,855,988 - PS417_27125 0.65 -2.9 5,855,988 - PS417_27125 0.65 -0.9 5,855,988 - PS417_27125 0.65 +1.0 5,855,988 - -0.8 5,856,060 - PS417_27125 0.71 -3.0 5,856,092 + PS417_27125 0.74 -2.4
Or see this region's nucleotide sequence