Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26310 and PS417_26315 are separated by 415 nucleotides PS417_26315 and PS417_26320 are separated by 68 nucleotides PS417_26320 and PS417_26325 overlap by 4 nucleotides
PS417_26310: PS417_26310 - serine dehydratase, at 5,685,430 to 5,686,806
_26310
PS417_26315: PS417_26315 - glycine/betaine ABC transporter substrate-binding protein, at 5,687,222 to 5,688,169
_26315
PS417_26320: PS417_26320 - ABC transporter permease, at 5,688,238 to 5,689,083
_26320
PS417_26325: PS417_26325 - ABC transporter ATP-binding protein, at 5,689,080 to 5,690,258
_26325
Position (kb) 5687
5688
5689 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5686.377 kb on - strand, within PS417_26310 at 5686.379 kb on + strand, within PS417_26310 at 5686.379 kb on + strand, within PS417_26310 at 5686.379 kb on + strand, within PS417_26310 at 5686.380 kb on - strand, within PS417_26310 at 5686.380 kb on - strand, within PS417_26310 at 5686.483 kb on + strand, within PS417_26310 at 5686.521 kb on + strand, within PS417_26310 at 5686.521 kb on + strand, within PS417_26310 at 5686.521 kb on + strand, within PS417_26310 at 5686.758 kb on + strand at 5686.759 kb on - strand at 5686.975 kb on - strand at 5687.061 kb on - strand at 5687.082 kb on - strand at 5687.082 kb on - strand at 5687.103 kb on - strand at 5687.174 kb on + strand at 5687.174 kb on + strand at 5687.175 kb on - strand at 5687.176 kb on + strand at 5687.177 kb on - strand at 5687.387 kb on + strand, within PS417_26315 at 5687.507 kb on + strand, within PS417_26315 at 5687.562 kb on - strand, within PS417_26315 at 5687.654 kb on + strand, within PS417_26315 at 5687.654 kb on + strand, within PS417_26315 at 5687.918 kb on + strand, within PS417_26315 at 5687.918 kb on + strand, within PS417_26315 at 5687.939 kb on + strand, within PS417_26315 at 5688.120 kb on + strand at 5688.120 kb on + strand at 5688.120 kb on + strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.121 kb on - strand at 5688.167 kb on + strand at 5688.234 kb on + strand at 5688.234 kb on + strand at 5688.235 kb on - strand at 5688.235 kb on - strand at 5688.264 kb on - strand at 5688.271 kb on + strand at 5688.271 kb on + strand at 5688.272 kb on - strand at 5688.272 kb on - strand at 5688.272 kb on - strand at 5688.316 kb on + strand at 5688.316 kb on + strand at 5688.317 kb on - strand at 5688.317 kb on - strand at 5688.439 kb on - strand, within PS417_26320 at 5688.439 kb on - strand, within PS417_26320 at 5688.439 kb on - strand, within PS417_26320 at 5688.604 kb on + strand, within PS417_26320 at 5688.604 kb on + strand, within PS417_26320 at 5688.605 kb on - strand, within PS417_26320 at 5688.715 kb on - strand, within PS417_26320 at 5688.755 kb on - strand, within PS417_26320 at 5689.063 kb on - strand at 5689.063 kb on - strand at 5689.068 kb on - strand at 5689.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,686,377 - PS417_26310 0.69 +1.0 5,686,379 + PS417_26310 0.69 -0.1 5,686,379 + PS417_26310 0.69 +0.4 5,686,379 + PS417_26310 0.69 +0.2 5,686,380 - PS417_26310 0.69 +2.0 5,686,380 - PS417_26310 0.69 -1.9 5,686,483 + PS417_26310 0.76 +0.8 5,686,521 + PS417_26310 0.79 -0.2 5,686,521 + PS417_26310 0.79 -0.2 5,686,521 + PS417_26310 0.79 +0.6 5,686,758 + +2.3 5,686,759 - +2.8 5,686,975 - +1.0 5,687,061 - -1.1 5,687,082 - -1.0 5,687,082 - -0.2 5,687,103 - -0.2 5,687,174 + +0.8 5,687,174 + +0.9 5,687,175 - -0.1 5,687,176 + +1.0 5,687,177 - -0.7 5,687,387 + PS417_26315 0.17 -1.9 5,687,507 + PS417_26315 0.30 -0.1 5,687,562 - PS417_26315 0.36 +0.9 5,687,654 + PS417_26315 0.46 +1.6 5,687,654 + PS417_26315 0.46 -0.6 5,687,918 + PS417_26315 0.73 +0.4 5,687,918 + PS417_26315 0.73 -0.4 5,687,939 + PS417_26315 0.76 -1.2 5,688,120 + -0.3 5,688,120 + +0.5 5,688,120 + -1.8 5,688,121 - +0.8 5,688,121 - +0.3 5,688,121 - +0.5 5,688,121 - +0.7 5,688,167 + +1.1 5,688,234 + +1.7 5,688,234 + +0.2 5,688,235 - +0.2 5,688,235 - -0.4 5,688,264 - +0.1 5,688,271 + -1.9 5,688,271 + +0.5 5,688,272 - +0.7 5,688,272 - -0.6 5,688,272 - -0.6 5,688,316 + +0.4 5,688,316 + -0.3 5,688,317 - -0.1 5,688,317 - -0.0 5,688,439 - PS417_26320 0.24 -0.3 5,688,439 - PS417_26320 0.24 -0.2 5,688,439 - PS417_26320 0.24 +0.8 5,688,604 + PS417_26320 0.43 -1.2 5,688,604 + PS417_26320 0.43 -0.9 5,688,605 - PS417_26320 0.43 -0.5 5,688,715 - PS417_26320 0.56 -0.4 5,688,755 - PS417_26320 0.61 +0.6 5,689,063 - +1.0 5,689,063 - -0.4 5,689,068 - -0.5 5,689,068 - +0.1
Or see this region's nucleotide sequence