Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25815 and PS417_25820 overlap by 1 nucleotides PS417_25820 and PS417_25825 are separated by 9 nucleotides PS417_25825 and PS417_25830 are separated by 284 nucleotides
PS417_25815: PS417_25815 - magnesium transporter ApaG, at 5,574,760 to 5,575,140
_25815
PS417_25820: PS417_25820 - diadenosine tetraphosphatase, at 5,575,140 to 5,575,991
_25820
PS417_25825: PS417_25825 - sulfurtransferase, at 5,576,001 to 5,576,330
_25825
PS417_25830: PS417_25830 - serine/threonine protein kinase, at 5,576,615 to 5,578,537
_25830
Position (kb)
5576
5577 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5575.002 kb on + strand, within PS417_25815 at 5575.003 kb on - strand, within PS417_25815 at 5575.003 kb on - strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.006 kb on + strand, within PS417_25815 at 5575.007 kb on - strand, within PS417_25815 at 5575.934 kb on + strand at 5575.935 kb on - strand at 5575.965 kb on - strand at 5575.989 kb on + strand at 5576.018 kb on + strand at 5576.018 kb on + strand at 5576.091 kb on + strand, within PS417_25825 at 5576.193 kb on + strand, within PS417_25825 at 5576.256 kb on + strand, within PS417_25825 at 5576.257 kb on - strand, within PS417_25825 at 5576.257 kb on - strand, within PS417_25825 at 5576.257 kb on - strand, within PS417_25825 at 5576.402 kb on + strand at 5576.403 kb on - strand at 5576.440 kb on + strand at 5576.441 kb on - strand at 5576.441 kb on - strand at 5576.593 kb on + strand at 5576.620 kb on + strand at 5576.718 kb on - strand at 5576.718 kb on - strand at 5576.934 kb on - strand, within PS417_25830 at 5577.012 kb on - strand, within PS417_25830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,575,002 + PS417_25815 0.64 +1.4 5,575,003 - PS417_25815 0.64 -1.4 5,575,003 - PS417_25815 0.64 +0.7 5,575,006 + PS417_25815 0.65 -2.6 5,575,006 + PS417_25815 0.65 +0.2 5,575,006 + PS417_25815 0.65 +0.9 5,575,006 + PS417_25815 0.65 +1.7 5,575,007 - PS417_25815 0.65 +0.2 5,575,934 + -1.6 5,575,935 - -1.0 5,575,965 - +2.0 5,575,989 + +0.2 5,576,018 + -0.1 5,576,018 + +0.0 5,576,091 + PS417_25825 0.27 -0.6 5,576,193 + PS417_25825 0.58 -1.1 5,576,256 + PS417_25825 0.77 -2.0 5,576,257 - PS417_25825 0.78 -1.2 5,576,257 - PS417_25825 0.78 -0.2 5,576,257 - PS417_25825 0.78 +0.0 5,576,402 + +1.1 5,576,403 - +0.9 5,576,440 + +0.5 5,576,441 - +1.1 5,576,441 - -0.8 5,576,593 + -0.8 5,576,620 + -0.9 5,576,718 - -0.4 5,576,718 - -0.2 5,576,934 - PS417_25830 0.17 -1.1 5,577,012 - PS417_25830 0.21 +0.7
Or see this region's nucleotide sequence