Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25275 and PS417_25280 overlap by 4 nucleotides PS417_25280 and PS417_25285 are separated by 151 nucleotides PS417_25285 and PS417_25290 overlap by 4 nucleotides PS417_25290 and PS417_25295 are separated by 130 nucleotides
PS417_25275: PS417_25275 - hypothetical protein, at 5,475,005 to 5,475,460
_25275
PS417_25280: PS417_25280 - methyltransferase, at 5,475,457 to 5,476,113
_25280
PS417_25285: PS417_25285 - thioredoxin, at 5,476,265 to 5,477,137
_25285
PS417_25290: PS417_25290 - hypothetical protein, at 5,477,134 to 5,477,481
_25290
PS417_25295: PS417_25295 - hypothetical protein, at 5,477,612 to 5,478,214
_25295
Position (kb) 5476
5477
5478 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5475.267 kb on + strand, within PS417_25275 at 5475.267 kb on + strand, within PS417_25275 at 5475.268 kb on - strand, within PS417_25275 at 5475.552 kb on - strand, within PS417_25280 at 5475.671 kb on - strand, within PS417_25280 at 5475.853 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5476.085 kb on + strand at 5476.086 kb on - strand at 5476.093 kb on + strand at 5476.228 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.250 kb on + strand at 5476.250 kb on + strand at 5476.283 kb on + strand at 5476.283 kb on + strand at 5476.427 kb on + strand, within PS417_25285 at 5476.427 kb on + strand, within PS417_25285 at 5476.428 kb on - strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.602 kb on + strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.603 kb on - strand, within PS417_25285 at 5476.718 kb on - strand, within PS417_25285 at 5476.941 kb on - strand, within PS417_25285 at 5477.027 kb on + strand, within PS417_25285 at 5477.118 kb on + strand at 5477.118 kb on + strand at 5477.119 kb on - strand at 5477.132 kb on + strand at 5477.135 kb on + strand at 5477.153 kb on + strand at 5477.153 kb on + strand at 5477.323 kb on + strand, within PS417_25290 at 5477.324 kb on - strand, within PS417_25290 at 5477.324 kb on - strand, within PS417_25290 at 5477.489 kb on - strand at 5477.572 kb on - strand at 5477.894 kb on - strand, within PS417_25295 at 5477.894 kb on - strand, within PS417_25295 at 5478.054 kb on - strand, within PS417_25295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,475,267 + PS417_25275 0.57 -1.0 5,475,267 + PS417_25275 0.57 -1.9 5,475,268 - PS417_25275 0.58 -0.5 5,475,552 - PS417_25280 0.14 +0.6 5,475,671 - PS417_25280 0.33 -1.1 5,475,853 + PS417_25280 0.60 +0.5 5,475,916 + PS417_25280 0.70 +0.8 5,475,916 + PS417_25280 0.70 +1.7 5,476,085 + -1.0 5,476,086 - -0.7 5,476,093 + -0.9 5,476,228 + +0.4 5,476,230 + +0.5 5,476,230 + -0.7 5,476,230 + +0.3 5,476,231 - +0.2 5,476,231 - +0.8 5,476,231 - -2.2 5,476,231 - +0.0 5,476,250 + -0.3 5,476,250 + -1.4 5,476,283 + +0.7 5,476,283 + +1.1 5,476,427 + PS417_25285 0.19 -1.8 5,476,427 + PS417_25285 0.19 -0.5 5,476,428 - PS417_25285 0.19 +0.2 5,476,602 + PS417_25285 0.39 +0.2 5,476,602 + PS417_25285 0.39 +0.4 5,476,602 + PS417_25285 0.39 -1.2 5,476,602 + PS417_25285 0.39 +1.7 5,476,603 - PS417_25285 0.39 +0.7 5,476,603 - PS417_25285 0.39 +1.1 5,476,603 - PS417_25285 0.39 -0.1 5,476,603 - PS417_25285 0.39 +1.5 5,476,603 - PS417_25285 0.39 -0.7 5,476,603 - PS417_25285 0.39 +1.0 5,476,603 - PS417_25285 0.39 +1.9 5,476,603 - PS417_25285 0.39 +0.1 5,476,718 - PS417_25285 0.52 +0.6 5,476,941 - PS417_25285 0.77 -1.5 5,477,027 + PS417_25285 0.87 +0.6 5,477,118 + -2.4 5,477,118 + +0.9 5,477,119 - -1.1 5,477,132 + -3.8 5,477,135 + -0.5 5,477,153 + -0.2 5,477,153 + -1.0 5,477,323 + PS417_25290 0.54 +0.7 5,477,324 - PS417_25290 0.55 +0.0 5,477,324 - PS417_25290 0.55 +0.2 5,477,489 - -1.2 5,477,572 - +0.0 5,477,894 - PS417_25295 0.47 -0.4 5,477,894 - PS417_25295 0.47 -1.2 5,478,054 - PS417_25295 0.73 +0.2
Or see this region's nucleotide sequence