Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25265 and PS417_25270 overlap by 4 nucleotides PS417_25270 and PS417_25275 are separated by 140 nucleotides PS417_25275 and PS417_25280 overlap by 4 nucleotides PS417_25280 and PS417_25285 are separated by 151 nucleotides
PS417_25265: PS417_25265 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 5,473,267 to 5,474,403
_25265
PS417_25270: PS417_25270 - NrdR family transcriptional regulator, at 5,474,400 to 5,474,864
_25270
PS417_25275: PS417_25275 - hypothetical protein, at 5,475,005 to 5,475,460
_25275
PS417_25280: PS417_25280 - methyltransferase, at 5,475,457 to 5,476,113
_25280
PS417_25285: PS417_25285 - thioredoxin, at 5,476,265 to 5,477,137
_25285
Position (kb) 5475
5476 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.495 kb on - strand, within PS417_25270 at 5474.725 kb on - strand, within PS417_25270 at 5474.883 kb on - strand at 5474.883 kb on - strand at 5474.883 kb on - strand at 5474.935 kb on - strand at 5474.939 kb on + strand at 5474.939 kb on + strand at 5474.940 kb on - strand at 5474.940 kb on - strand at 5474.941 kb on + strand at 5474.942 kb on - strand at 5474.942 kb on - strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.943 kb on + strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.944 kb on - strand at 5474.953 kb on - strand at 5474.955 kb on + strand at 5474.956 kb on - strand at 5474.956 kb on - strand at 5475.002 kb on + strand at 5475.002 kb on + strand at 5475.002 kb on + strand at 5475.003 kb on - strand at 5475.003 kb on - strand at 5475.003 kb on - strand at 5475.267 kb on + strand, within PS417_25275 at 5475.267 kb on + strand, within PS417_25275 at 5475.268 kb on - strand, within PS417_25275 at 5475.552 kb on - strand, within PS417_25280 at 5475.671 kb on - strand, within PS417_25280 at 5475.853 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5475.916 kb on + strand, within PS417_25280 at 5476.085 kb on + strand at 5476.086 kb on - strand at 5476.093 kb on + strand at 5476.228 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.230 kb on + strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.231 kb on - strand at 5476.250 kb on + strand at 5476.250 kb on + strand at 5476.283 kb on + strand at 5476.283 kb on + strand at 5476.427 kb on + strand, within PS417_25285 at 5476.427 kb on + strand, within PS417_25285 at 5476.428 kb on - strand, within PS417_25285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,474,495 - PS417_25270 0.20 +0.6 5,474,495 - PS417_25270 0.20 -0.9 5,474,495 - PS417_25270 0.20 +0.3 5,474,495 - PS417_25270 0.20 -0.5 5,474,495 - PS417_25270 0.20 -0.5 5,474,725 - PS417_25270 0.70 -2.9 5,474,883 - +1.2 5,474,883 - +0.8 5,474,883 - -0.3 5,474,935 - -1.1 5,474,939 + -0.4 5,474,939 + +0.7 5,474,940 - -1.4 5,474,940 - -0.2 5,474,941 + +1.0 5,474,942 - -0.6 5,474,942 - -1.9 5,474,943 + -0.6 5,474,943 + -0.3 5,474,943 + +0.4 5,474,943 + +0.5 5,474,944 - -0.1 5,474,944 - +0.6 5,474,944 - +0.7 5,474,944 - -0.1 5,474,953 - -0.1 5,474,955 + -1.7 5,474,956 - +1.8 5,474,956 - -0.2 5,475,002 + -0.8 5,475,002 + +1.0 5,475,002 + +0.2 5,475,003 - -0.3 5,475,003 - +0.4 5,475,003 - -0.9 5,475,267 + PS417_25275 0.57 -1.0 5,475,267 + PS417_25275 0.57 -1.9 5,475,268 - PS417_25275 0.58 -0.5 5,475,552 - PS417_25280 0.14 +0.6 5,475,671 - PS417_25280 0.33 -1.1 5,475,853 + PS417_25280 0.60 +0.5 5,475,916 + PS417_25280 0.70 +0.8 5,475,916 + PS417_25280 0.70 +1.7 5,476,085 + -1.0 5,476,086 - -0.7 5,476,093 + -0.9 5,476,228 + +0.4 5,476,230 + +0.5 5,476,230 + -0.7 5,476,230 + +0.3 5,476,231 - +0.2 5,476,231 - +0.8 5,476,231 - -2.2 5,476,231 - +0.0 5,476,250 + -0.3 5,476,250 + -1.4 5,476,283 + +0.7 5,476,283 + +1.1 5,476,427 + PS417_25285 0.19 -1.8 5,476,427 + PS417_25285 0.19 -0.5 5,476,428 - PS417_25285 0.19 +0.2
Or see this region's nucleotide sequence