Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24780 and PS417_24785 are separated by 29 nucleotides PS417_24785 and PS417_24790 are separated by 138 nucleotides PS417_24790 and PS417_24795 overlap by 8 nucleotides PS417_24795 and PS417_24800 overlap by 4 nucleotides
PS417_24780: PS417_24780 - peptidase C13, at 5,373,806 to 5,375,524
_24780
PS417_24785: PS417_24785 - nucleoside-diphosphate sugar epimerase, at 5,375,554 to 5,376,195
_24785
PS417_24790: PS417_24790 - hypothetical protein, at 5,376,334 to 5,376,618
_24790
PS417_24795: PS417_24795 - aromatic acid decarboxylase, at 5,376,611 to 5,377,240
_24795
PS417_24800: PS417_24800 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 5,377,237 to 5,378,586
_24800
Position (kb)
5376
5377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5375.451 kb on + strand at 5375.500 kb on + strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.537 kb on - strand at 5375.557 kb on + strand at 5375.558 kb on - strand at 5375.558 kb on - strand at 5375.574 kb on + strand at 5375.575 kb on - strand at 5375.926 kb on + strand, within PS417_24785 at 5375.927 kb on - strand at 5375.927 kb on - strand, within PS417_24785 at 5376.082 kb on + strand, within PS417_24785 at 5376.193 kb on + strand at 5376.193 kb on + strand at 5376.194 kb on - strand at 5376.194 kb on - strand at 5376.203 kb on + strand at 5376.204 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.522 kb on - strand, within PS417_24790 at 5377.570 kb on - strand, within PS417_24800 at 5377.608 kb on - strand, within PS417_24800 at 5377.608 kb on - strand, within PS417_24800 at 5377.608 kb on - strand, within PS417_24800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,375,451 + +0.9 5,375,500 + -0.7 5,375,523 - +1.2 5,375,523 - +1.0 5,375,523 - +0.7 5,375,523 - +0.1 5,375,537 - +0.2 5,375,557 + +0.0 5,375,558 - -0.6 5,375,558 - -0.5 5,375,574 + +1.4 5,375,575 - +3.3 5,375,926 + PS417_24785 0.58 +0.3 5,375,927 - -1.3 5,375,927 - PS417_24785 0.58 +0.2 5,376,082 + PS417_24785 0.82 -3.0 5,376,193 + +0.0 5,376,193 + +1.3 5,376,194 - +1.0 5,376,194 - -0.2 5,376,203 + -1.8 5,376,204 - +0.0 5,376,272 - -1.3 5,376,272 - +0.1 5,376,272 - -0.2 5,376,272 - -0.0 5,376,274 + +0.0 5,376,274 + +1.0 5,376,274 + +0.1 5,376,521 + PS417_24790 0.66 +1.6 5,376,521 + PS417_24790 0.66 -0.1 5,376,521 + PS417_24790 0.66 -1.1 5,376,521 + PS417_24790 0.66 -1.2 5,376,521 + PS417_24790 0.66 +0.1 5,376,522 - PS417_24790 0.66 -0.2 5,377,570 - PS417_24800 0.25 -1.9 5,377,608 - PS417_24800 0.27 -0.4 5,377,608 - PS417_24800 0.27 -1.4 5,377,608 - PS417_24800 0.27 +0.0
Or see this region's nucleotide sequence