Strain Fitness in Pseudomonas simiae WCS417 around PS417_24500

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_24495 and PS417_24500 are separated by 123 nucleotidesPS417_24500 and PS417_24505 are separated by 48 nucleotides PS417_24495: PS417_24495 - NAD(FAD)-utilizing dehydrogenase, at 5,316,210 to 5,317,436 _24495 PS417_24500: PS417_24500 - DEAD/DEAH box helicase, at 5,317,560 to 5,318,894 _24500 PS417_24505: PS417_24505 - membrane protein, at 5,318,943 to 5,319,839 _24505 Position (kb) 5317 5318 5319Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 5316.646 kb on + strand, within PS417_24495at 5316.647 kb on - strand, within PS417_24495at 5316.781 kb on - strand, within PS417_24495at 5316.846 kb on - strand, within PS417_24495at 5317.197 kb on - strand, within PS417_24495at 5317.369 kb on + strandat 5317.370 kb on - strandat 5317.501 kb on + strandat 5317.501 kb on + strandat 5317.502 kb on - strandat 5317.912 kb on + strand, within PS417_24500at 5317.912 kb on + strand, within PS417_24500at 5317.912 kb on + strand, within PS417_24500at 5317.913 kb on - strand, within PS417_24500at 5318.378 kb on - strand, within PS417_24500at 5318.578 kb on + strand, within PS417_24500at 5318.896 kb on + strandat 5318.930 kb on - strandat 5318.944 kb on + strandat 5318.945 kb on - strandat 5318.949 kb on + strandat 5318.950 kb on - strandat 5319.094 kb on + strand, within PS417_24505at 5319.094 kb on + strand, within PS417_24505at 5319.094 kb on + strand, within PS417_24505at 5319.094 kb on + strand, within PS417_24505at 5319.094 kb on + strand, within PS417_24505at 5319.095 kb on - strand, within PS417_24505at 5319.100 kb on + strand, within PS417_24505at 5319.100 kb on + strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.101 kb on - strand, within PS417_24505at 5319.139 kb on + strand, within PS417_24505at 5319.139 kb on + strand, within PS417_24505at 5319.139 kb on + strand, within PS417_24505at 5319.140 kb on - strand, within PS417_24505at 5319.145 kb on + strand, within PS417_24505at 5319.145 kb on + strand, within PS417_24505at 5319.145 kb on + strand, within PS417_24505at 5319.145 kb on + strand, within PS417_24505at 5319.145 kb on + strand, within PS417_24505at 5319.146 kb on - strandat 5319.146 kb on - strand, within PS417_24505at 5319.146 kb on - strand, within PS417_24505at 5319.146 kb on - strand, within PS417_24505at 5319.146 kb on - strand, within PS417_24505at 5319.146 kb on - strand, within PS417_24505at 5319.146 kb on - strand, within PS417_24505at 5319.184 kb on + strand, within PS417_24505at 5319.185 kb on - strand, within PS417_24505at 5319.404 kb on + strand, within PS417_24505at 5319.404 kb on + strand, within PS417_24505at 5319.404 kb on + strand, within PS417_24505at 5319.404 kb on + strand, within PS417_24505at 5319.404 kb on + strand, within PS417_24505at 5319.405 kb on - strand, within PS417_24505at 5319.405 kb on - strand, within PS417_24505at 5319.474 kb on - strand, within PS417_24505at 5319.491 kb on - strand, within PS417_24505at 5319.498 kb on + strand, within PS417_24505at 5319.498 kb on + strandat 5319.498 kb on + strand, within PS417_24505at 5319.498 kb on + strand, within PS417_24505at 5319.499 kb on - strand, within PS417_24505at 5319.499 kb on - strand, within PS417_24505at 5319.499 kb on - strand, within PS417_24505at 5319.499 kb on - strand, within PS417_24505at 5319.764 kb on - strandat 5319.764 kb on - strandat 5319.764 kb on - strandat 5319.767 kb on + strandat 5319.768 kb on - strandat 5319.768 kb on - strandat 5319.784 kb on + strandat 5319.784 kb on + strandat 5319.784 kb on + strandat 5319.785 kb on - strandat 5319.850 kb on + strandat 5319.851 kb on - strandat 5319.871 kb on + strandat 5319.871 kb on + strandat 5319.871 kb on + strandat 5319.876 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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5,316,646 + PS417_24495 0.36 +0.4
5,316,647 - PS417_24495 0.36 +1.1
5,316,781 - PS417_24495 0.47 +0.0
5,316,846 - PS417_24495 0.52 -1.4
5,317,197 - PS417_24495 0.80 -0.1
5,317,369 + -0.3
5,317,370 - -1.8
5,317,501 + +1.2
5,317,501 + -0.1
5,317,502 - +0.4
5,317,912 + PS417_24500 0.26 -1.1
5,317,912 + PS417_24500 0.26 -2.0
5,317,912 + PS417_24500 0.26 -0.3
5,317,913 - PS417_24500 0.26 -3.2
5,318,378 - PS417_24500 0.61 +0.1
5,318,578 + PS417_24500 0.76 -3.2
5,318,896 + -0.3
5,318,930 - -0.0
5,318,944 + +0.1
5,318,945 - +0.6
5,318,949 + -0.2
5,318,950 - -0.4
5,319,094 + PS417_24505 0.17 +0.4
5,319,094 + PS417_24505 0.17 +0.9
5,319,094 + PS417_24505 0.17 +1.0
5,319,094 + PS417_24505 0.17 -0.2
5,319,094 + PS417_24505 0.17 -1.7
5,319,095 - PS417_24505 0.17 +0.7
5,319,100 + PS417_24505 0.18 +0.5
5,319,100 + PS417_24505 0.18 -0.6
5,319,101 - PS417_24505 0.18 +0.4
5,319,101 - PS417_24505 0.18 -0.1
5,319,101 - PS417_24505 0.18 -0.9
5,319,101 - PS417_24505 0.18 +0.3
5,319,101 - PS417_24505 0.18 -1.7
5,319,101 - PS417_24505 0.18 +0.8
5,319,101 - PS417_24505 0.18 +2.9
5,319,101 - PS417_24505 0.18 +0.6
5,319,139 + PS417_24505 0.22 +0.6
5,319,139 + PS417_24505 0.22 -0.5
5,319,139 + PS417_24505 0.22 -0.3
5,319,140 - PS417_24505 0.22 +0.2
5,319,145 + PS417_24505 0.23 -1.2
5,319,145 + PS417_24505 0.23 -0.4
5,319,145 + PS417_24505 0.23 +1.1
5,319,145 + PS417_24505 0.23 +0.1
5,319,145 + PS417_24505 0.23 -1.3
5,319,146 - +1.3
5,319,146 - PS417_24505 0.23 -1.1
5,319,146 - PS417_24505 0.23 -0.1
5,319,146 - PS417_24505 0.23 -0.9
5,319,146 - PS417_24505 0.23 +0.2
5,319,146 - PS417_24505 0.23 +0.3
5,319,146 - PS417_24505 0.23 +0.0
5,319,184 + PS417_24505 0.27 -0.2
5,319,185 - PS417_24505 0.27 +0.8
5,319,404 + PS417_24505 0.51 +2.3
5,319,404 + PS417_24505 0.51 -0.2
5,319,404 + PS417_24505 0.51 +0.8
5,319,404 + PS417_24505 0.51 +0.2
5,319,404 + PS417_24505 0.51 -1.1
5,319,405 - PS417_24505 0.52 -0.8
5,319,405 - PS417_24505 0.52 +0.1
5,319,474 - PS417_24505 0.59 -0.0
5,319,491 - PS417_24505 0.61 -1.2
5,319,498 + PS417_24505 0.62 +0.7
5,319,498 + -0.3
5,319,498 + PS417_24505 0.62 -0.5
5,319,498 + PS417_24505 0.62 +0.1
5,319,499 - PS417_24505 0.62 +1.7
5,319,499 - PS417_24505 0.62 +0.2
5,319,499 - PS417_24505 0.62 -0.2
5,319,499 - PS417_24505 0.62 -0.8
5,319,764 - +0.9
5,319,764 - +0.5
5,319,764 - -0.6
5,319,767 + +1.1
5,319,768 - -1.3
5,319,768 - +4.5
5,319,784 + -0.6
5,319,784 + +1.2
5,319,784 + -0.1
5,319,785 - -0.0
5,319,850 + -1.0
5,319,851 - -1.9
5,319,871 + +1.0
5,319,871 + +0.7
5,319,871 + -0.7
5,319,876 + -1.1

Or see this region's nucleotide sequence