Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24240 and PS417_24245 are separated by 171 nucleotides PS417_24245 and PS417_24250 are separated by 132 nucleotides PS417_24250 and PS417_24255 are separated by 70 nucleotides PS417_24255 and PS417_24260 overlap by 4 nucleotides
PS417_24240: PS417_24240 - membrane protein, at 5,258,354 to 5,260,207
_24240
PS417_24245: PS417_24245 - chorismate mutase, at 5,260,379 to 5,260,678
_24245
PS417_24250: PS417_24250 - hypothetical protein, at 5,260,811 to 5,261,167
_24250
PS417_24255: PS417_24255 - elongation factor GreAB, at 5,261,238 to 5,261,735
_24255
PS417_24260: PS417_24260 - glutamate dehydrogenase, at 5,261,732 to 5,263,069
_24260
Position (kb) 5260
5261
5262 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5260.145 kb on + strand at 5260.197 kb on - strand at 5260.203 kb on - strand at 5260.263 kb on - strand at 5260.334 kb on - strand at 5260.334 kb on - strand at 5260.341 kb on + strand at 5260.342 kb on - strand at 5260.342 kb on - strand at 5260.473 kb on - strand, within PS417_24245 at 5260.703 kb on + strand at 5260.737 kb on + strand at 5260.737 kb on + strand at 5260.738 kb on - strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.770 kb on - strand at 5260.805 kb on + strand at 5261.194 kb on - strand at 5261.223 kb on + strand at 5261.223 kb on + strand at 5261.224 kb on - strand at 5261.382 kb on + strand, within PS417_24255 at 5261.382 kb on + strand, within PS417_24255 at 5261.383 kb on - strand, within PS417_24255 at 5261.383 kb on - strand, within PS417_24255 at 5261.454 kb on + strand, within PS417_24255 at 5261.516 kb on - strand, within PS417_24255 at 5261.516 kb on - strand, within PS417_24255 at 5261.733 kb on + strand at 5261.802 kb on + strand at 5261.803 kb on - strand at 5261.803 kb on - strand at 5261.803 kb on - strand at 5262.055 kb on + strand, within PS417_24260 at 5262.055 kb on + strand, within PS417_24260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,260,145 + -2.1 5,260,197 - +0.1 5,260,203 - +0.2 5,260,263 - +1.4 5,260,334 - +0.5 5,260,334 - -0.0 5,260,341 + +0.8 5,260,342 - +0.5 5,260,342 - +3.5 5,260,473 - PS417_24245 0.31 +0.0 5,260,703 + -0.1 5,260,737 + -0.2 5,260,737 + +1.9 5,260,738 - -0.8 5,260,769 + +2.2 5,260,769 + -0.3 5,260,769 + +0.6 5,260,769 + -0.3 5,260,770 - -3.2 5,260,805 + +0.3 5,261,194 - -0.9 5,261,223 + +1.0 5,261,223 + +1.1 5,261,224 - -0.5 5,261,382 + PS417_24255 0.29 -0.1 5,261,382 + PS417_24255 0.29 +0.4 5,261,383 - PS417_24255 0.29 +0.7 5,261,383 - PS417_24255 0.29 -0.3 5,261,454 + PS417_24255 0.43 -0.9 5,261,516 - PS417_24255 0.56 +0.3 5,261,516 - PS417_24255 0.56 +0.4 5,261,733 + +1.2 5,261,802 + -2.6 5,261,803 - -0.2 5,261,803 - -0.6 5,261,803 - -1.1 5,262,055 + PS417_24260 0.24 +0.1 5,262,055 + PS417_24260 0.24 +2.6
Or see this region's nucleotide sequence