Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24220 and PS417_24225 are separated by 3 nucleotides PS417_24225 and PS417_24230 are separated by 178 nucleotides PS417_24230 and PS417_24235 are separated by 267 nucleotides PS417_24235 and PS417_24240 are separated by 91 nucleotides
PS417_24220: PS417_24220 - hypothetical protein, at 5,252,757 to 5,254,577
_24220
PS417_24225: PS417_24225 - hypothetical protein, at 5,254,581 to 5,254,889
_24225
PS417_24230: PS417_24230 - ATP-dependent protease, at 5,255,068 to 5,257,506
_24230
PS417_24235: PS417_24235 - hypothetical protein, at 5,257,774 to 5,258,262
_24235
PS417_24240: PS417_24240 - membrane protein, at 5,258,354 to 5,260,207
_24240
Position (kb)
5255
5256
5257
5258 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.204 kb on - strand, within PS417_24220 at 5254.380 kb on + strand, within PS417_24220 at 5254.380 kb on + strand, within PS417_24220 at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.405 kb on - strand at 5254.405 kb on - strand at 5254.446 kb on + strand at 5254.473 kb on + strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.479 kb on + strand at 5254.480 kb on - strand at 5254.663 kb on + strand, within PS417_24225 at 5254.664 kb on - strand, within PS417_24225 at 5255.279 kb on + strand at 5255.279 kb on + strand at 5255.279 kb on + strand at 5255.571 kb on - strand, within PS417_24230 at 5255.625 kb on - strand, within PS417_24230 at 5255.625 kb on - strand, within PS417_24230 at 5256.044 kb on + strand, within PS417_24230 at 5256.224 kb on + strand, within PS417_24230 at 5256.228 kb on - strand, within PS417_24230 at 5256.363 kb on + strand, within PS417_24230 at 5256.474 kb on - strand, within PS417_24230 at 5256.524 kb on + strand, within PS417_24230 at 5256.678 kb on - strand, within PS417_24230 at 5256.698 kb on + strand, within PS417_24230 at 5256.726 kb on - strand, within PS417_24230 at 5257.223 kb on + strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.517 kb on + strand at 5257.518 kb on - strand at 5257.518 kb on - strand at 5257.673 kb on + strand at 5257.674 kb on - strand at 5257.699 kb on + strand at 5257.699 kb on + strand at 5257.700 kb on - strand at 5257.700 kb on - strand at 5257.703 kb on - strand at 5257.703 kb on - strand at 5257.774 kb on - strand at 5257.926 kb on + strand, within PS417_24235 at 5257.927 kb on - strand, within PS417_24235 at 5257.997 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.260 kb on + strand at 5258.261 kb on - strand at 5258.331 kb on + strand at 5258.342 kb on + strand at 5258.343 kb on - strand at 5258.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,254,203 + PS417_24220 0.79 -1.7 5,254,203 + PS417_24220 0.79 -0.1 5,254,203 + PS417_24220 0.79 +0.7 5,254,203 + PS417_24220 0.79 +0.0 5,254,203 + PS417_24220 0.79 +0.2 5,254,203 + PS417_24220 0.79 -0.1 5,254,203 + PS417_24220 0.79 +0.4 5,254,203 + PS417_24220 0.79 +0.7 5,254,204 - PS417_24220 0.79 -1.1 5,254,380 + PS417_24220 0.89 +0.7 5,254,380 + PS417_24220 0.89 +0.3 5,254,404 + +2.6 5,254,404 + +0.8 5,254,404 + +0.3 5,254,404 + -0.1 5,254,405 - +0.1 5,254,405 - -0.3 5,254,446 + +0.6 5,254,473 + +1.6 5,254,474 - -1.1 5,254,474 - +0.4 5,254,474 - -0.4 5,254,474 - -0.1 5,254,479 + +0.6 5,254,480 - -0.3 5,254,663 + PS417_24225 0.27 -1.5 5,254,664 - PS417_24225 0.27 +0.2 5,255,279 + -0.6 5,255,279 + -2.8 5,255,279 + -0.8 5,255,571 - PS417_24230 0.21 -1.7 5,255,625 - PS417_24230 0.23 +1.3 5,255,625 - PS417_24230 0.23 -2.1 5,256,044 + PS417_24230 0.40 -1.3 5,256,224 + PS417_24230 0.47 +0.2 5,256,228 - PS417_24230 0.48 -0.5 5,256,363 + PS417_24230 0.53 -2.6 5,256,474 - PS417_24230 0.58 -2.1 5,256,524 + PS417_24230 0.60 -2.0 5,256,678 - PS417_24230 0.66 -2.5 5,256,698 + PS417_24230 0.67 -1.3 5,256,726 - PS417_24230 0.68 -2.3 5,257,223 + PS417_24230 0.88 -1.3 5,257,224 - PS417_24230 0.88 +1.2 5,257,224 - PS417_24230 0.88 -1.4 5,257,224 - PS417_24230 0.88 +1.7 5,257,224 - PS417_24230 0.88 -1.3 5,257,224 - PS417_24230 0.88 -0.2 5,257,517 + +1.3 5,257,518 - +0.9 5,257,518 - -0.6 5,257,673 + +1.7 5,257,674 - -0.3 5,257,699 + +0.9 5,257,699 + -2.3 5,257,700 - -1.0 5,257,700 - +0.2 5,257,703 - +0.2 5,257,703 - -0.6 5,257,774 - -2.2 5,257,926 + PS417_24235 0.31 -0.7 5,257,927 - PS417_24235 0.31 -2.5 5,257,997 - PS417_24235 0.46 -3.3 5,258,087 - PS417_24235 0.64 -0.1 5,258,087 - PS417_24235 0.64 +0.6 5,258,260 + -1.5 5,258,261 - +0.4 5,258,331 + +0.3 5,258,342 + +0.2 5,258,343 - -0.3 5,258,354 - +0.2
Or see this region's nucleotide sequence