Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23620 and PS417_23625 are separated by 103 nucleotides PS417_23625 and PS417_23630 are separated by 30 nucleotides PS417_23630 and PS417_23635 are separated by 120 nucleotides
PS417_23620: PS417_23620 - epimerase, at 5,108,711 to 5,109,604
_23620
PS417_23625: PS417_23625 - LysR family transcriptional regulator, at 5,109,708 to 5,110,604
_23625
PS417_23630: PS417_23630 - NADPH quinone reductase MdaB, at 5,110,635 to 5,111,225
_23630
PS417_23635: PS417_23635 - membrane protein, at 5,111,346 to 5,112,110
_23635
Position (kb)
5109
5110
5111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5108.749 kb on + strand at 5108.750 kb on - strand at 5108.750 kb on - strand at 5108.820 kb on + strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.991 kb on + strand, within PS417_23620 at 5108.992 kb on - strand, within PS417_23620 at 5109.258 kb on + strand, within PS417_23620 at 5109.259 kb on - strand, within PS417_23620 at 5109.259 kb on - strand at 5109.591 kb on - strand at 5109.596 kb on + strand at 5109.597 kb on - strand at 5109.642 kb on - strand at 5109.665 kb on + strand at 5109.665 kb on + strand at 5109.666 kb on - strand at 5109.884 kb on + strand, within PS417_23625 at 5109.900 kb on - strand, within PS417_23625 at 5109.900 kb on - strand, within PS417_23625 at 5110.122 kb on - strand, within PS417_23625 at 5110.274 kb on - strand, within PS417_23625 at 5110.581 kb on + strand at 5110.581 kb on + strand at 5110.582 kb on - strand at 5110.636 kb on + strand at 5110.636 kb on + strand at 5110.783 kb on + strand, within PS417_23630 at 5110.783 kb on + strand, within PS417_23630 at 5110.783 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.870 kb on + strand, within PS417_23630 at 5110.871 kb on - strand, within PS417_23630 at 5110.871 kb on - strand, within PS417_23630 at 5110.903 kb on + strand, within PS417_23630 at 5110.903 kb on + strand, within PS417_23630 at 5110.984 kb on + strand, within PS417_23630 at 5111.032 kb on + strand, within PS417_23630 at 5111.032 kb on + strand, within PS417_23630 at 5111.033 kb on - strand, within PS417_23630 at 5111.083 kb on + strand, within PS417_23630 at 5111.167 kb on + strand at 5111.309 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,108,749 + +0.2 5,108,750 - -1.3 5,108,750 - +0.2 5,108,820 + PS417_23620 0.12 +0.1 5,108,821 - PS417_23620 0.12 -0.4 5,108,821 - PS417_23620 0.12 +0.8 5,108,821 - PS417_23620 0.12 -1.8 5,108,821 - PS417_23620 0.12 +1.4 5,108,991 + PS417_23620 0.31 -0.8 5,108,992 - PS417_23620 0.31 -0.2 5,109,258 + PS417_23620 0.61 -0.2 5,109,259 - PS417_23620 0.61 +0.8 5,109,259 - -0.2 5,109,591 - -3.5 5,109,596 + +0.3 5,109,597 - -0.2 5,109,642 - +0.4 5,109,665 + +0.4 5,109,665 + +2.8 5,109,666 - -1.1 5,109,884 + PS417_23625 0.20 -1.9 5,109,900 - PS417_23625 0.21 -2.9 5,109,900 - PS417_23625 0.21 -0.8 5,110,122 - PS417_23625 0.46 +0.2 5,110,274 - PS417_23625 0.63 -0.3 5,110,581 + -0.6 5,110,581 + -0.9 5,110,582 - +0.9 5,110,636 + +0.6 5,110,636 + +0.3 5,110,783 + PS417_23630 0.25 -3.0 5,110,783 + PS417_23630 0.25 -1.3 5,110,783 + PS417_23630 0.25 -0.4 5,110,870 + PS417_23630 0.40 +1.8 5,110,870 + PS417_23630 0.40 +0.1 5,110,870 + PS417_23630 0.40 -0.2 5,110,870 + PS417_23630 0.40 -1.2 5,110,870 + PS417_23630 0.40 -1.8 5,110,871 - PS417_23630 0.40 +0.0 5,110,871 - PS417_23630 0.40 -1.2 5,110,903 + PS417_23630 0.45 +0.2 5,110,903 + PS417_23630 0.45 -2.1 5,110,984 + PS417_23630 0.59 -0.2 5,111,032 + PS417_23630 0.67 -1.2 5,111,032 + PS417_23630 0.67 -0.2 5,111,033 - PS417_23630 0.67 -0.5 5,111,083 + PS417_23630 0.76 +0.5 5,111,167 + -0.3 5,111,309 + -2.2
Or see this region's nucleotide sequence