Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23405 and PS417_23410 are separated by 71 nucleotides PS417_23410 and PS417_23415 are separated by 138 nucleotides
PS417_23405: PS417_23405 - transcriptional regulator, at 5,066,822 to 5,068,345
_23405
PS417_23410: PS417_23410 - membrane protein, at 5,068,417 to 5,069,328
_23410
PS417_23415: PS417_23415 - AraC family transcriptional regulator, at 5,069,467 to 5,070,342
_23415
Position (kb)
5068
5069
5070 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5068.013 kb on + strand, within PS417_23405 at 5068.013 kb on + strand, within PS417_23405 at 5068.014 kb on - strand, within PS417_23405 at 5068.220 kb on + strand at 5068.332 kb on + strand at 5068.343 kb on + strand at 5068.344 kb on - strand at 5068.381 kb on + strand at 5068.382 kb on - strand at 5068.550 kb on + strand, within PS417_23410 at 5068.551 kb on - strand, within PS417_23410 at 5068.694 kb on + strand, within PS417_23410 at 5068.694 kb on + strand, within PS417_23410 at 5068.695 kb on - strand, within PS417_23410 at 5068.742 kb on + strand, within PS417_23410 at 5068.743 kb on - strand, within PS417_23410 at 5068.766 kb on + strand, within PS417_23410 at 5068.767 kb on - strand at 5068.977 kb on + strand, within PS417_23410 at 5069.095 kb on + strand, within PS417_23410 at 5069.096 kb on - strand, within PS417_23410 at 5069.308 kb on + strand at 5069.313 kb on - strand at 5069.459 kb on + strand at 5069.632 kb on + strand, within PS417_23415 at 5069.633 kb on - strand, within PS417_23415 at 5069.768 kb on - strand, within PS417_23415 at 5069.927 kb on + strand, within PS417_23415 at 5070.049 kb on + strand, within PS417_23415 at 5070.049 kb on + strand, within PS417_23415 at 5070.050 kb on - strand, within PS417_23415 at 5070.169 kb on + strand, within PS417_23415 at 5070.169 kb on + strand, within PS417_23415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,068,013 + PS417_23405 0.78 -1.6 5,068,013 + PS417_23405 0.78 +0.2 5,068,014 - PS417_23405 0.78 +0.3 5,068,220 + -1.0 5,068,332 + +0.4 5,068,343 + +0.7 5,068,344 - -2.7 5,068,381 + +0.1 5,068,382 - +1.4 5,068,550 + PS417_23410 0.15 +0.5 5,068,551 - PS417_23410 0.15 +1.0 5,068,694 + PS417_23410 0.30 +3.0 5,068,694 + PS417_23410 0.30 -1.0 5,068,695 - PS417_23410 0.30 -0.7 5,068,742 + PS417_23410 0.36 -1.5 5,068,743 - PS417_23410 0.36 +2.8 5,068,766 + PS417_23410 0.38 +0.0 5,068,767 - +1.8 5,068,977 + PS417_23410 0.61 +1.0 5,069,095 + PS417_23410 0.74 -2.2 5,069,096 - PS417_23410 0.74 +0.4 5,069,308 + +0.3 5,069,313 - +0.1 5,069,459 + +0.3 5,069,632 + PS417_23415 0.19 -1.1 5,069,633 - PS417_23415 0.19 -0.8 5,069,768 - PS417_23415 0.34 -0.6 5,069,927 + PS417_23415 0.53 +0.3 5,070,049 + PS417_23415 0.66 -0.2 5,070,049 + PS417_23415 0.66 +0.2 5,070,050 - PS417_23415 0.67 -0.6 5,070,169 + PS417_23415 0.80 -0.3 5,070,169 + PS417_23415 0.80 +0.1
Or see this region's nucleotide sequence