Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22225 and PS417_22230 overlap by 4 nucleotides PS417_22230 and PS417_22235 are separated by 111 nucleotides PS417_22235 and PS417_22240 are separated by 638 nucleotides PS417_22240 and PS417_22245 are separated by 73 nucleotides
PS417_22225: PS417_22225 - MFS transporter, at 4,817,204 to 4,818,400
_22225
PS417_22230: PS417_22230 - class I peptide chain release factor, at 4,818,397 to 4,818,810
_22230
PS417_22235: PS417_22235 - aromatic amino acid transporter, at 4,818,922 to 4,820,343
_22235
PS417_22240: PS417_22240 - mercuric reductase, at 4,820,982 to 4,821,119
_22240
PS417_22245: PS417_22245 - leucyl aminopeptidase (aminopeptidase T), at 4,821,193 to 4,822,152
_22245
Position (kb)
4818
4819
4820
4821 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4817.922 kb on - strand, within PS417_22225 at 4817.922 kb on - strand, within PS417_22225 at 4817.922 kb on - strand, within PS417_22225 at 4818.353 kb on + strand at 4818.353 kb on + strand at 4818.655 kb on - strand, within PS417_22230 at 4818.655 kb on - strand, within PS417_22230 at 4818.656 kb on + strand, within PS417_22230 at 4818.656 kb on + strand, within PS417_22230 at 4818.657 kb on - strand, within PS417_22230 at 4818.657 kb on - strand, within PS417_22230 at 4818.657 kb on - strand, within PS417_22230 at 4818.810 kb on + strand at 4818.865 kb on + strand at 4818.966 kb on + strand at 4819.097 kb on + strand, within PS417_22235 at 4819.097 kb on + strand, within PS417_22235 at 4819.097 kb on + strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.098 kb on - strand, within PS417_22235 at 4819.205 kb on - strand, within PS417_22235 at 4819.262 kb on + strand, within PS417_22235 at 4819.262 kb on + strand, within PS417_22235 at 4819.262 kb on + strand, within PS417_22235 at 4819.403 kb on + strand, within PS417_22235 at 4819.403 kb on + strand, within PS417_22235 at 4819.403 kb on + strand, within PS417_22235 at 4819.404 kb on - strand, within PS417_22235 at 4819.418 kb on + strand, within PS417_22235 at 4819.418 kb on + strand, within PS417_22235 at 4819.418 kb on + strand, within PS417_22235 at 4819.419 kb on - strand, within PS417_22235 at 4819.419 kb on - strand, within PS417_22235 at 4819.518 kb on - strand, within PS417_22235 at 4819.518 kb on - strand, within PS417_22235 at 4819.599 kb on - strand, within PS417_22235 at 4819.617 kb on - strand, within PS417_22235 at 4819.617 kb on - strand, within PS417_22235 at 4819.617 kb on - strand, within PS417_22235 at 4819.985 kb on + strand, within PS417_22235 at 4819.986 kb on - strand, within PS417_22235 at 4819.995 kb on - strand, within PS417_22235 at 4819.995 kb on - strand, within PS417_22235 at 4819.995 kb on - strand, within PS417_22235 at 4820.036 kb on + strand, within PS417_22235 at 4820.087 kb on + strand, within PS417_22235 at 4820.087 kb on + strand, within PS417_22235 at 4820.088 kb on - strand, within PS417_22235 at 4820.088 kb on - strand, within PS417_22235 at 4820.220 kb on + strand at 4820.220 kb on + strand at 4820.286 kb on + strand at 4820.446 kb on + strand at 4820.446 kb on + strand at 4820.446 kb on + strand at 4820.695 kb on + strand at 4820.741 kb on + strand at 4820.911 kb on - strand at 4820.976 kb on + strand at 4820.977 kb on - strand at 4821.118 kb on - strand at 4821.226 kb on + strand at 4821.227 kb on - strand at 4821.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,817,922 - PS417_22225 0.60 -1.3 4,817,922 - PS417_22225 0.60 +1.7 4,817,922 - PS417_22225 0.60 +0.1 4,818,353 + +2.0 4,818,353 + +1.6 4,818,655 - PS417_22230 0.62 -0.5 4,818,655 - PS417_22230 0.62 -0.1 4,818,656 + PS417_22230 0.63 -0.4 4,818,656 + PS417_22230 0.63 -0.7 4,818,657 - PS417_22230 0.63 -0.4 4,818,657 - PS417_22230 0.63 +0.3 4,818,657 - PS417_22230 0.63 +0.8 4,818,810 + +0.3 4,818,865 + -0.7 4,818,966 + -1.2 4,819,097 + PS417_22235 0.12 +0.6 4,819,097 + PS417_22235 0.12 +0.4 4,819,097 + PS417_22235 0.12 +1.2 4,819,098 - PS417_22235 0.12 +0.2 4,819,098 - PS417_22235 0.12 +0.2 4,819,098 - PS417_22235 0.12 -0.2 4,819,098 - PS417_22235 0.12 -2.2 4,819,098 - PS417_22235 0.12 +0.7 4,819,098 - PS417_22235 0.12 -0.1 4,819,098 - PS417_22235 0.12 +1.7 4,819,098 - PS417_22235 0.12 +1.4 4,819,205 - PS417_22235 0.20 -0.4 4,819,262 + PS417_22235 0.24 -1.2 4,819,262 + PS417_22235 0.24 -1.0 4,819,262 + PS417_22235 0.24 +0.9 4,819,403 + PS417_22235 0.34 +0.0 4,819,403 + PS417_22235 0.34 +0.5 4,819,403 + PS417_22235 0.34 +0.9 4,819,404 - PS417_22235 0.34 +0.9 4,819,418 + PS417_22235 0.35 +1.3 4,819,418 + PS417_22235 0.35 +1.4 4,819,418 + PS417_22235 0.35 +1.1 4,819,419 - PS417_22235 0.35 -2.0 4,819,419 - PS417_22235 0.35 -0.4 4,819,518 - PS417_22235 0.42 +0.1 4,819,518 - PS417_22235 0.42 -0.5 4,819,599 - PS417_22235 0.48 +1.1 4,819,617 - PS417_22235 0.49 -0.2 4,819,617 - PS417_22235 0.49 +1.0 4,819,617 - PS417_22235 0.49 -0.1 4,819,985 + PS417_22235 0.75 +0.7 4,819,986 - PS417_22235 0.75 +0.2 4,819,995 - PS417_22235 0.75 +0.4 4,819,995 - PS417_22235 0.75 -0.7 4,819,995 - PS417_22235 0.75 +2.0 4,820,036 + PS417_22235 0.78 -3.0 4,820,087 + PS417_22235 0.82 -0.0 4,820,087 + PS417_22235 0.82 +0.8 4,820,088 - PS417_22235 0.82 -0.4 4,820,088 - PS417_22235 0.82 +0.6 4,820,220 + +0.1 4,820,220 + +1.5 4,820,286 + +1.0 4,820,446 + +0.5 4,820,446 + -1.2 4,820,446 + -0.9 4,820,695 + +0.6 4,820,741 + -0.3 4,820,911 - -0.2 4,820,976 + -1.3 4,820,977 - +0.3 4,821,118 - +0.4 4,821,226 + -0.6 4,821,227 - +1.1 4,821,230 - +1.0
Or see this region's nucleotide sequence