Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21805 and PS417_21810 are separated by 93 nucleotides PS417_21810 and PS417_21815 are separated by 223 nucleotides PS417_21815 and PS417_21820 are separated by 97 nucleotides PS417_21820 and PS417_21825 are separated by 327 nucleotides
PS417_21805: PS417_21805 - hypothetical protein, at 4,735,130 to 4,736,077
_21805
PS417_21810: PS417_21810 - hypothetical protein, at 4,736,171 to 4,736,536
_21810
PS417_21815: PS417_21815 - amidase, at 4,736,760 to 4,738,262
_21815
PS417_21820: PS417_21820 - hypothetical protein, at 4,738,360 to 4,738,902
_21820
PS417_21825: PS417_21825 - hydrolase, at 4,739,230 to 4,740,279
_21825
Position (kb)
4736
4737
4738
4739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4735.776 kb on - strand, within PS417_21805 at 4735.776 kb on - strand, within PS417_21805 at 4735.776 kb on - strand, within PS417_21805 at 4735.776 kb on - strand, within PS417_21805 at 4735.833 kb on - strand, within PS417_21805 at 4735.848 kb on - strand, within PS417_21805 at 4735.875 kb on - strand, within PS417_21805 at 4735.937 kb on + strand, within PS417_21805 at 4735.938 kb on - strand, within PS417_21805 at 4736.076 kb on - strand at 4736.136 kb on - strand at 4736.535 kb on - strand at 4736.535 kb on - strand at 4736.854 kb on + strand at 4736.855 kb on - strand at 4736.855 kb on - strand at 4736.855 kb on - strand at 4736.883 kb on + strand at 4736.884 kb on - strand at 4736.884 kb on - strand at 4736.935 kb on + strand, within PS417_21815 at 4736.936 kb on - strand, within PS417_21815 at 4737.181 kb on + strand, within PS417_21815 at 4737.181 kb on + strand, within PS417_21815 at 4737.185 kb on + strand, within PS417_21815 at 4737.185 kb on + strand, within PS417_21815 at 4737.185 kb on + strand, within PS417_21815 at 4737.216 kb on + strand, within PS417_21815 at 4737.217 kb on - strand, within PS417_21815 at 4737.226 kb on + strand, within PS417_21815 at 4737.402 kb on + strand, within PS417_21815 at 4737.402 kb on + strand, within PS417_21815 at 4737.412 kb on + strand, within PS417_21815 at 4737.412 kb on + strand, within PS417_21815 at 4737.419 kb on + strand, within PS417_21815 at 4737.596 kb on + strand, within PS417_21815 at 4737.597 kb on - strand, within PS417_21815 at 4737.597 kb on - strand, within PS417_21815 at 4737.597 kb on - strand, within PS417_21815 at 4738.153 kb on + strand at 4738.215 kb on + strand at 4738.215 kb on + strand at 4738.262 kb on + strand at 4738.262 kb on + strand at 4738.262 kb on + strand at 4738.263 kb on - strand at 4738.263 kb on - strand at 4738.263 kb on - strand at 4738.438 kb on + strand, within PS417_21820 at 4738.870 kb on + strand at 4738.871 kb on - strand at 4738.995 kb on + strand at 4738.995 kb on + strand at 4738.996 kb on - strand at 4739.083 kb on + strand at 4739.083 kb on + strand at 4739.083 kb on + strand at 4739.084 kb on - strand at 4739.086 kb on - strand at 4739.112 kb on + strand at 4739.112 kb on + strand at 4739.112 kb on + strand at 4739.112 kb on + strand at 4739.121 kb on + strand at 4739.126 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,735,776 - PS417_21805 0.68 +1.5 4,735,776 - PS417_21805 0.68 +1.6 4,735,776 - PS417_21805 0.68 +0.6 4,735,776 - PS417_21805 0.68 +0.1 4,735,833 - PS417_21805 0.74 -0.8 4,735,848 - PS417_21805 0.76 -0.1 4,735,875 - PS417_21805 0.79 -0.2 4,735,937 + PS417_21805 0.85 -0.4 4,735,938 - PS417_21805 0.85 -1.6 4,736,076 - -0.5 4,736,136 - +0.2 4,736,535 - -1.1 4,736,535 - -0.3 4,736,854 + +0.3 4,736,855 - +1.8 4,736,855 - +0.7 4,736,855 - +1.1 4,736,883 + +0.1 4,736,884 - -0.0 4,736,884 - -0.5 4,736,935 + PS417_21815 0.12 +1.2 4,736,936 - PS417_21815 0.12 +0.0 4,737,181 + PS417_21815 0.28 +0.5 4,737,181 + PS417_21815 0.28 +0.4 4,737,185 + PS417_21815 0.28 -0.1 4,737,185 + PS417_21815 0.28 -0.3 4,737,185 + PS417_21815 0.28 -0.2 4,737,216 + PS417_21815 0.30 +0.9 4,737,217 - PS417_21815 0.30 +1.5 4,737,226 + PS417_21815 0.31 -2.4 4,737,402 + PS417_21815 0.43 -2.9 4,737,402 + PS417_21815 0.43 -0.2 4,737,412 + PS417_21815 0.43 +1.3 4,737,412 + PS417_21815 0.43 -0.1 4,737,419 + PS417_21815 0.44 -0.9 4,737,596 + PS417_21815 0.56 +0.8 4,737,597 - PS417_21815 0.56 -2.5 4,737,597 - PS417_21815 0.56 -1.2 4,737,597 - PS417_21815 0.56 -0.5 4,738,153 + -2.1 4,738,215 + -0.6 4,738,215 + +0.2 4,738,262 + +0.8 4,738,262 + -0.4 4,738,262 + -0.0 4,738,263 - -1.7 4,738,263 - -0.0 4,738,263 - -1.9 4,738,438 + PS417_21820 0.14 -2.3 4,738,870 + +0.2 4,738,871 - -2.0 4,738,995 + +0.5 4,738,995 + +1.1 4,738,996 - +0.1 4,739,083 + -0.1 4,739,083 + +0.1 4,739,083 + -0.4 4,739,084 - -2.2 4,739,086 - +0.0 4,739,112 + +0.8 4,739,112 + -0.4 4,739,112 + -1.3 4,739,112 + -2.0 4,739,121 + -1.5 4,739,126 - +0.2
Or see this region's nucleotide sequence