Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02140 and PS417_02145 are separated by 48 nucleotides PS417_02145 and PS417_02150 are separated by 50 nucleotides
PS417_02140: PS417_02140 - hypothetical protein, at 475,709 to 477,172
_02140
PS417_02145: PS417_02145 - potassium transporter KefA, at 477,221 to 480,559
_02145
PS417_02150: PS417_02150 - potassium/proton antiporter, at 480,610 to 482,352
_02150
Position (kb) 477
478
479
480
481 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 476.449 kb on - strand, within PS417_02140 at 476.556 kb on - strand, within PS417_02140 at 476.598 kb on + strand, within PS417_02140 at 476.598 kb on + strand, within PS417_02140 at 476.601 kb on + strand, within PS417_02140 at 476.602 kb on - strand, within PS417_02140 at 476.602 kb on - strand, within PS417_02140 at 476.607 kb on + strand, within PS417_02140 at 476.607 kb on + strand, within PS417_02140 at 476.843 kb on + strand, within PS417_02140 at 476.944 kb on - strand, within PS417_02140 at 477.214 kb on - strand at 477.304 kb on - strand at 477.331 kb on - strand at 477.389 kb on - strand at 477.489 kb on + strand at 477.489 kb on + strand at 477.490 kb on - strand at 477.490 kb on - strand at 477.490 kb on - strand at 477.490 kb on - strand at 477.939 kb on + strand, within PS417_02145 at 477.939 kb on + strand, within PS417_02145 at 477.939 kb on + strand, within PS417_02145 at 477.940 kb on - strand, within PS417_02145 at 477.940 kb on - strand, within PS417_02145 at 477.940 kb on - strand, within PS417_02145 at 477.964 kb on - strand, within PS417_02145 at 478.106 kb on - strand, within PS417_02145 at 478.128 kb on + strand, within PS417_02145 at 478.128 kb on + strand, within PS417_02145 at 478.128 kb on + strand, within PS417_02145 at 478.129 kb on - strand, within PS417_02145 at 478.191 kb on + strand, within PS417_02145 at 478.191 kb on + strand, within PS417_02145 at 478.419 kb on + strand, within PS417_02145 at 478.809 kb on + strand, within PS417_02145 at 479.025 kb on + strand, within PS417_02145 at 479.110 kb on - strand, within PS417_02145 at 479.110 kb on - strand, within PS417_02145 at 479.436 kb on + strand, within PS417_02145 at 479.436 kb on + strand, within PS417_02145 at 479.437 kb on - strand, within PS417_02145 at 479.488 kb on - strand, within PS417_02145 at 479.561 kb on - strand, within PS417_02145 at 479.562 kb on + strand, within PS417_02145 at 479.562 kb on + strand, within PS417_02145 at 479.712 kb on + strand, within PS417_02145 at 479.713 kb on - strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.892 kb on - strand, within PS417_02145 at 480.373 kb on - strand at 480.373 kb on - strand at 480.373 kb on - strand at 480.554 kb on - strand at 480.559 kb on + strand at 480.559 kb on + strand at 480.611 kb on + strand at 480.982 kb on - strand, within PS417_02150 at 481.265 kb on + strand, within PS417_02150 at 481.266 kb on - strand, within PS417_02150 at 481.350 kb on + strand, within PS417_02150 at 481.471 kb on + strand, within PS417_02150 at 481.472 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.548 kb on + strand, within PS417_02150 at 481.549 kb on - strand, within PS417_02150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 476,449 - PS417_02140 0.51 -0.4 476,556 - PS417_02140 0.58 +0.5 476,598 + PS417_02140 0.61 -0.0 476,598 + PS417_02140 0.61 -1.2 476,601 + PS417_02140 0.61 +0.2 476,602 - PS417_02140 0.61 +0.1 476,602 - PS417_02140 0.61 +0.9 476,607 + PS417_02140 0.61 -0.4 476,607 + PS417_02140 0.61 -1.6 476,843 + PS417_02140 0.77 -0.2 476,944 - PS417_02140 0.84 -2.9 477,214 - -0.1 477,304 - -3.1 477,331 - -2.9 477,389 - -4.2 477,489 + -1.7 477,489 + -0.4 477,490 - -1.0 477,490 - +1.8 477,490 - -0.9 477,490 - -2.8 477,939 + PS417_02145 0.22 +1.8 477,939 + PS417_02145 0.22 -2.9 477,939 + PS417_02145 0.22 -2.7 477,940 - PS417_02145 0.22 -1.7 477,940 - PS417_02145 0.22 -2.4 477,940 - PS417_02145 0.22 -2.2 477,964 - PS417_02145 0.22 -1.2 478,106 - PS417_02145 0.27 -0.4 478,128 + PS417_02145 0.27 -3.4 478,128 + PS417_02145 0.27 +0.9 478,128 + PS417_02145 0.27 -1.3 478,129 - PS417_02145 0.27 +1.4 478,191 + PS417_02145 0.29 -3.5 478,191 + PS417_02145 0.29 -2.8 478,419 + PS417_02145 0.36 -2.7 478,809 + PS417_02145 0.48 -0.8 479,025 + PS417_02145 0.54 -3.3 479,110 - PS417_02145 0.57 -2.4 479,110 - PS417_02145 0.57 -0.6 479,436 + PS417_02145 0.66 +0.8 479,436 + PS417_02145 0.66 -2.9 479,437 - PS417_02145 0.66 -1.9 479,488 - PS417_02145 0.68 -4.8 479,561 - PS417_02145 0.70 -2.3 479,562 + PS417_02145 0.70 -0.2 479,562 + PS417_02145 0.70 -1.2 479,712 + PS417_02145 0.75 -2.0 479,713 - PS417_02145 0.75 -2.5 479,891 + PS417_02145 0.80 -0.2 479,891 + PS417_02145 0.80 -3.2 479,891 + PS417_02145 0.80 -4.9 479,892 - PS417_02145 0.80 -1.9 480,373 - +1.8 480,373 - -0.1 480,373 - -2.3 480,554 - -0.8 480,559 + -3.8 480,559 + -2.6 480,611 + -1.3 480,982 - PS417_02150 0.21 -1.4 481,265 + PS417_02150 0.38 -1.8 481,266 - PS417_02150 0.38 -2.1 481,350 + PS417_02150 0.42 -0.8 481,471 + PS417_02150 0.49 -2.6 481,472 - PS417_02150 0.49 -0.7 481,513 - PS417_02150 0.52 -2.6 481,513 - PS417_02150 0.52 -0.5 481,548 + PS417_02150 0.54 -3.3 481,549 - PS417_02150 0.54 -1.5
Or see this region's nucleotide sequence