Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01955 and PS417_01960 are separated by 218 nucleotides PS417_01960 and PS417_01965 overlap by 4 nucleotides PS417_01965 and PS417_01970 overlap by 4 nucleotides
PS417_01955: PS417_01955 - peptidase, at 433,512 to 435,974
_01955
PS417_01960: PS417_01960 - pilus assembly protein, at 436,193 to 436,846
_01960
PS417_01965: PS417_01965 - fimbriae biosynthesis protein, at 436,843 to 437,397
_01965
PS417_01970: PS417_01970 - pilus assembly protein, at 437,394 to 438,353
_01970
Position (kb)
436
437 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 435.217 kb on + strand at 435.218 kb on - strand, within PS417_01955 at 435.284 kb on - strand, within PS417_01955 at 435.421 kb on + strand, within PS417_01955 at 435.422 kb on - strand, within PS417_01955 at 435.424 kb on + strand, within PS417_01955 at 435.424 kb on + strand, within PS417_01955 at 435.469 kb on + strand, within PS417_01955 at 435.484 kb on + strand, within PS417_01955 at 435.555 kb on - strand, within PS417_01955 at 435.555 kb on - strand, within PS417_01955 at 435.707 kb on + strand, within PS417_01955 at 435.884 kb on + strand at 436.007 kb on - strand at 436.082 kb on + strand at 436.083 kb on - strand at 436.166 kb on - strand at 436.166 kb on - strand at 436.311 kb on - strand, within PS417_01960 at 436.375 kb on - strand, within PS417_01960 at 436.536 kb on - strand, within PS417_01960 at 436.536 kb on - strand, within PS417_01960 at 436.617 kb on + strand, within PS417_01960 at 436.617 kb on + strand, within PS417_01960 at 436.618 kb on - strand at 436.618 kb on - strand, within PS417_01960 at 436.862 kb on + strand at 436.862 kb on + strand at 436.863 kb on - strand at 436.903 kb on + strand, within PS417_01965 at 436.907 kb on + strand, within PS417_01965 at 436.907 kb on + strand, within PS417_01965 at 437.453 kb on - strand at 437.517 kb on + strand, within PS417_01970 at 437.517 kb on + strand, within PS417_01970 at 437.518 kb on - strand, within PS417_01970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 435,217 + -0.6 435,218 - PS417_01955 0.69 -0.8 435,284 - PS417_01955 0.72 +0.6 435,421 + PS417_01955 0.78 +1.6 435,422 - PS417_01955 0.78 -2.0 435,424 + PS417_01955 0.78 +0.6 435,424 + PS417_01955 0.78 +0.4 435,469 + PS417_01955 0.79 +0.1 435,484 + PS417_01955 0.80 -2.0 435,555 - PS417_01955 0.83 +1.1 435,555 - PS417_01955 0.83 -1.0 435,707 + PS417_01955 0.89 +0.5 435,884 + +0.4 436,007 - +0.6 436,082 + -1.0 436,083 - -0.1 436,166 - +0.0 436,166 - -1.6 436,311 - PS417_01960 0.18 +0.2 436,375 - PS417_01960 0.28 -1.5 436,536 - PS417_01960 0.52 -2.5 436,536 - PS417_01960 0.52 -1.6 436,617 + PS417_01960 0.65 -0.6 436,617 + PS417_01960 0.65 -2.0 436,618 - +1.5 436,618 - PS417_01960 0.65 -0.8 436,862 + +0.3 436,862 + +0.4 436,863 - +3.3 436,903 + PS417_01965 0.11 -1.0 436,907 + PS417_01965 0.12 -1.8 436,907 + PS417_01965 0.12 -0.6 437,453 - -0.8 437,517 + PS417_01970 0.13 -0.8 437,517 + PS417_01970 0.13 -0.1 437,518 - PS417_01970 0.13 +0.2
Or see this region's nucleotide sequence