Strain Fitness in Pseudomonas simiae WCS417 around PS417_18445

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.003 mg/ml
4,011,171	+		PS417_18430	0.37	+0.6
4,011,434	+				-0.7
4,011,434	+				+0.7
4,011,434	+				+0.4
4,011,435	-				-0.9
4,011,547	+		PS417_18435	0.18	+1.9
4,011,716	+				+0.1
4,011,717	-		PS417_18435	0.89	-0.2
4,011,773	+				-1.5
4,011,777	+				+0.4
4,011,777	+				-0.0
4,011,778	-				+0.9
4,011,797	+				-1.6
4,011,797	+				+0.7
4,011,797	+				+0.2
4,011,798	-				-0.4
4,011,798	-				+1.2
4,011,798	-				+0.3
4,011,798	-				+0.7
4,011,911	-		PS417_18440	0.24	+1.2
4,011,911	-		PS417_18440	0.24	-0.7
4,011,911	-		PS417_18440	0.24	-0.5
4,011,911	-				+1.7
4,011,915	+		PS417_18440	0.26	+0.7
4,011,915	+		PS417_18440	0.26	-0.3
4,011,916	-		PS417_18440	0.26	+1.0
4,012,174	+				+0.2
4,012,175	-				-0.4
4,012,200	+				-3.4
4,012,201	-				-0.6
4,012,207	-				-0.4
4,012,207	-				-0.1
4,012,216	+				-1.6
4,012,314	+		PS417_18445	0.24	-0.9
4,012,381	+		PS417_18445	0.34	+1.6
4,012,496	+		PS417_18445	0.51	-0.1
4,012,804	+				-0.6
4,012,804	+				+0.5
4,012,804	+				-0.4
4,012,804	+				+0.1
4,012,804	+				-0.2
4,012,879	-				+1.5
4,012,879	-				+0.2
4,012,970	+				-0.3
4,012,970	+				-0.6
4,013,014	+				-0.9
4,013,383	-		PS417_18450	0.45	+2.0
4,013,440	-		PS417_18450	0.52	-0.6
4,013,518	-		PS417_18450	0.62	-1.7
4,013,518	-		PS417_18450	0.62	-0.4
4,013,559	+				+0.1
4,013,559	+		PS417_18450	0.68	-0.5
4,013,559	+		PS417_18450	0.68	-0.8
4,013,560	-		PS417_18450	0.68	-0.0
4,013,805	+				-0.8
4,013,806	-				-0.7
4,013,817	+				-2.5
4,013,817	+				+0.0