Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01710 and PS417_01715 overlap by 4 nucleotides PS417_01715 and PS417_01720 are separated by 405 nucleotides PS417_01720 and PS417_01725 are separated by 141 nucleotides
PS417_01710: PS417_01710 - histidine utilization repressor, at 379,017 to 379,766
_01710
PS417_01715: PS417_01715 - hypothetical protein, at 379,763 to 380,323
_01715
PS417_01720: PS417_01720 - Urocanate hydratase (EC 4.2.1.49) (from data) , at 380,729 to 382,399
_01720
PS417_01725: PS417_01725 - sulfonate ABC transporter substrate-binding protein, at 382,541 to 383,998
_01725
Position (kb)
380
381
382
383 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 379.796 kb on + strand at 379.797 kb on - strand at 379.800 kb on + strand at 379.801 kb on - strand at 379.937 kb on + strand, within PS417_01715 at 380.255 kb on - strand, within PS417_01715 at 380.372 kb on - strand at 380.372 kb on - strand at 380.550 kb on + strand at 380.567 kb on - strand at 380.648 kb on + strand at 380.659 kb on - strand at 380.659 kb on - strand at 380.740 kb on + strand at 380.741 kb on - strand at 380.883 kb on - strand at 381.113 kb on + strand, within PS417_01720 at 381.113 kb on + strand, within PS417_01720 at 381.113 kb on + strand, within PS417_01720 at 381.114 kb on - strand, within PS417_01720 at 381.114 kb on - strand, within PS417_01720 at 381.114 kb on - strand, within PS417_01720 at 381.114 kb on - strand, within PS417_01720 at 381.114 kb on - strand, within PS417_01720 at 381.143 kb on + strand, within PS417_01720 at 381.144 kb on - strand, within PS417_01720 at 381.176 kb on + strand, within PS417_01720 at 381.177 kb on - strand at 381.209 kb on + strand, within PS417_01720 at 381.209 kb on + strand, within PS417_01720 at 381.210 kb on - strand, within PS417_01720 at 381.413 kb on + strand, within PS417_01720 at 381.773 kb on + strand, within PS417_01720 at 381.849 kb on - strand, within PS417_01720 at 382.398 kb on - strand at 382.398 kb on - strand at 382.402 kb on - strand at 382.442 kb on + strand at 382.601 kb on + strand at 382.602 kb on - strand at 382.632 kb on + strand at 382.633 kb on - strand at 382.633 kb on - strand at 382.636 kb on + strand at 382.850 kb on + strand, within PS417_01725 at 382.889 kb on + strand, within PS417_01725 at 382.889 kb on + strand, within PS417_01725 at 382.890 kb on - strand, within PS417_01725 at 382.917 kb on + strand, within PS417_01725 at 383.223 kb on - strand, within PS417_01725 at 383.363 kb on + strand, within PS417_01725 at 383.364 kb on - strand, within PS417_01725 at 383.364 kb on - strand, within PS417_01725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 379,796 + -0.4 379,797 - -0.1 379,800 + -0.6 379,801 - -1.3 379,937 + PS417_01715 0.31 -0.0 380,255 - PS417_01715 0.88 -0.2 380,372 - +0.6 380,372 - -2.3 380,550 + -0.2 380,567 - -0.8 380,648 + -0.7 380,659 - -3.0 380,659 - -0.5 380,740 + -1.3 380,741 - +3.1 380,883 - +0.8 381,113 + PS417_01720 0.23 -0.5 381,113 + PS417_01720 0.23 -1.4 381,113 + PS417_01720 0.23 -0.9 381,114 - PS417_01720 0.23 -2.5 381,114 - PS417_01720 0.23 +0.7 381,114 - PS417_01720 0.23 -3.6 381,114 - PS417_01720 0.23 -1.7 381,114 - PS417_01720 0.23 -1.8 381,143 + PS417_01720 0.25 -0.1 381,144 - PS417_01720 0.25 -0.2 381,176 + PS417_01720 0.27 -2.6 381,177 - -0.2 381,209 + PS417_01720 0.29 -0.7 381,209 + PS417_01720 0.29 -0.2 381,210 - PS417_01720 0.29 -0.1 381,413 + PS417_01720 0.41 -1.0 381,773 + PS417_01720 0.62 -1.2 381,849 - PS417_01720 0.67 +0.8 382,398 - -0.3 382,398 - -0.5 382,402 - +0.1 382,442 + -2.3 382,601 + -0.5 382,602 - -0.0 382,632 + +0.0 382,633 - -0.4 382,633 - -1.4 382,636 + -0.4 382,850 + PS417_01725 0.21 +0.3 382,889 + PS417_01725 0.24 +1.5 382,889 + PS417_01725 0.24 -0.4 382,890 - PS417_01725 0.24 +2.1 382,917 + PS417_01725 0.26 +0.1 383,223 - PS417_01725 0.47 -1.0 383,363 + PS417_01725 0.56 +0.6 383,364 - PS417_01725 0.56 -3.9 383,364 - PS417_01725 0.56 -2.0
Or see this region's nucleotide sequence