Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16840 and PS417_16845 overlap by 4 nucleotides PS417_16845 and PS417_16850 overlap by 4 nucleotides PS417_16850 and PS417_16855 overlap by 4 nucleotides PS417_16855 and PS417_16860 are separated by 126 nucleotides
PS417_16840: PS417_16840 - monovalent cation/H+ antiporter subunit D, at 3,673,876 to 3,675,558
_16840
PS417_16845: PS417_16845 - monovalent cation/H+ antiporter subunit E, at 3,675,555 to 3,676,043
_16845
PS417_16850: PS417_16850 - cation:proton antiporter, at 3,676,040 to 3,676,318
_16850
PS417_16855: PS417_16855 - cation:proton antiporter, at 3,676,315 to 3,676,653
_16855
PS417_16860: PS417_16860 - diadenosine tetraphosphate hydrolase, at 3,676,780 to 3,677,238
_16860
Position (kb)
3676
3677 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3675.364 kb on + strand, within PS417_16840 at 3675.440 kb on - strand at 3675.481 kb on + strand at 3675.481 kb on + strand at 3675.695 kb on - strand, within PS417_16845 at 3675.766 kb on + strand, within PS417_16845 at 3675.767 kb on - strand, within PS417_16845 at 3675.914 kb on + strand at 3676.011 kb on + strand at 3676.011 kb on + strand at 3676.011 kb on + strand at 3676.012 kb on - strand at 3676.012 kb on - strand at 3676.163 kb on + strand, within PS417_16850 at 3676.164 kb on - strand, within PS417_16850 at 3676.226 kb on + strand, within PS417_16850 at 3676.358 kb on + strand, within PS417_16855 at 3676.577 kb on + strand, within PS417_16855 at 3676.723 kb on + strand at 3676.724 kb on - strand at 3676.778 kb on + strand at 3676.842 kb on - strand at 3676.857 kb on + strand, within PS417_16860 at 3676.857 kb on + strand, within PS417_16860 at 3676.860 kb on - strand, within PS417_16860 at 3676.860 kb on - strand, within PS417_16860 at 3676.915 kb on - strand, within PS417_16860 at 3677.094 kb on - strand, within PS417_16860 at 3677.167 kb on - strand, within PS417_16860 at 3677.212 kb on - strand at 3677.274 kb on - strand at 3677.274 kb on - strand at 3677.274 kb on - strand at 3677.301 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,675,364 + PS417_16840 0.88 -0.3 3,675,440 - +3.8 3,675,481 + -1.5 3,675,481 + +0.8 3,675,695 - PS417_16845 0.29 -0.8 3,675,766 + PS417_16845 0.43 +0.2 3,675,767 - PS417_16845 0.43 -0.8 3,675,914 + +0.4 3,676,011 + -1.0 3,676,011 + -0.2 3,676,011 + -0.8 3,676,012 - -2.6 3,676,012 - -0.4 3,676,163 + PS417_16850 0.44 +0.1 3,676,164 - PS417_16850 0.44 +2.1 3,676,226 + PS417_16850 0.67 -0.7 3,676,358 + PS417_16855 0.13 -0.7 3,676,577 + PS417_16855 0.77 +0.2 3,676,723 + +0.3 3,676,724 - +2.1 3,676,778 + +0.4 3,676,842 - -1.3 3,676,857 + PS417_16860 0.17 -0.0 3,676,857 + PS417_16860 0.17 -1.6 3,676,860 - PS417_16860 0.17 -1.0 3,676,860 - PS417_16860 0.17 -0.8 3,676,915 - PS417_16860 0.29 -0.4 3,677,094 - PS417_16860 0.68 -1.5 3,677,167 - PS417_16860 0.84 -0.7 3,677,212 - +0.9 3,677,274 - +0.7 3,677,274 - -0.8 3,677,274 - -0.8 3,677,301 + -0.5
Or see this region's nucleotide sequence