Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16805 and PS417_16810 overlap by 1 nucleotides PS417_16810 and PS417_16815 are separated by 11 nucleotides PS417_16815 and PS417_16820 are separated by 154 nucleotides PS417_16820 and PS417_16825 are separated by 92 nucleotides
PS417_16805: PS417_16805 - 5,10-methylene tetrahydromethanopterin reductase, at 3,665,750 to 3,667,129
_16805
PS417_16810: PS417_16810 - acyl-CoA dehydrogenase, at 3,667,129 to 3,667,455
_16810
PS417_16815: PS417_16815 - acyl-CoA dehydrogenase, at 3,667,467 to 3,668,663
_16815
PS417_16820: PS417_16820 - Cro/Cl family transcriptional regulator, at 3,668,818 to 3,669,381
_16820
PS417_16825: PS417_16825 - membrane protein, at 3,669,474 to 3,670,403
_16825
Position (kb)
3667
3668
3669 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3666.624 kb on + strand, within PS417_16805 at 3666.625 kb on - strand, within PS417_16805 at 3666.639 kb on + strand, within PS417_16805 at 3666.934 kb on - strand, within PS417_16805 at 3666.942 kb on + strand, within PS417_16805 at 3666.943 kb on - strand, within PS417_16805 at 3667.032 kb on + strand at 3667.151 kb on + strand at 3667.151 kb on + strand at 3667.151 kb on + strand at 3667.152 kb on - strand at 3667.382 kb on + strand, within PS417_16810 at 3667.383 kb on - strand, within PS417_16810 at 3667.468 kb on + strand at 3667.468 kb on + strand at 3668.323 kb on + strand, within PS417_16815 at 3668.453 kb on - strand, within PS417_16815 at 3668.500 kb on - strand, within PS417_16815 at 3668.633 kb on + strand at 3668.772 kb on + strand at 3668.971 kb on + strand, within PS417_16820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,666,624 + PS417_16805 0.63 -1.0 3,666,625 - PS417_16805 0.63 -0.2 3,666,639 + PS417_16805 0.64 +0.5 3,666,934 - PS417_16805 0.86 +0.2 3,666,942 + PS417_16805 0.86 -0.5 3,666,943 - PS417_16805 0.86 -0.7 3,667,032 + +3.5 3,667,151 + -1.2 3,667,151 + -0.4 3,667,151 + +0.5 3,667,152 - +0.0 3,667,382 + PS417_16810 0.77 -0.2 3,667,383 - PS417_16810 0.78 +0.3 3,667,468 + +1.1 3,667,468 + +0.5 3,668,323 + PS417_16815 0.72 -0.8 3,668,453 - PS417_16815 0.82 -0.8 3,668,500 - PS417_16815 0.86 -2.2 3,668,633 + -1.2 3,668,772 + +1.3 3,668,971 + PS417_16820 0.27 +2.4
Or see this region's nucleotide sequence