Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16655 and PS417_16660 are separated by 120 nucleotides PS417_16660 and PS417_16665 are separated by 31 nucleotides PS417_16665 and PS417_16675 are separated by 304 nucleotides PS417_16675 and PS417_16680 are separated by 41 nucleotides
PS417_16655: PS417_16655 - N-hydroxyarylamine O-acetyltransferase, at 3,639,394 to 3,640,221
_16655
PS417_16660: PS417_16660 - hypothetical protein, at 3,640,342 to 3,640,578
_16660
PS417_16665: PS417_16665 - hypothetical protein, at 3,640,610 to 3,640,810
_16665
PS417_16675: PS417_16675 - hypothetical protein, at 3,641,115 to 3,641,636
_16675
PS417_16680: PS417_16680 - MFS transporter, at 3,641,678 to 3,642,808
_16680
Position (kb)
3641
3642 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3640.256 kb on + strand at 3640.256 kb on + strand at 3640.320 kb on + strand at 3640.320 kb on + strand at 3640.320 kb on + strand at 3640.321 kb on - strand at 3640.321 kb on - strand at 3640.321 kb on - strand at 3640.372 kb on + strand, within PS417_16660 at 3640.612 kb on - strand at 3640.639 kb on - strand, within PS417_16665 at 3640.991 kb on + strand at 3640.992 kb on - strand at 3640.992 kb on - strand at 3641.024 kb on - strand at 3641.024 kb on - strand at 3641.027 kb on + strand at 3641.027 kb on + strand at 3641.028 kb on - strand at 3641.033 kb on + strand at 3641.169 kb on - strand, within PS417_16675 at 3641.169 kb on - strand, within PS417_16675 at 3641.169 kb on - strand, within PS417_16675 at 3641.171 kb on + strand, within PS417_16675 at 3641.171 kb on + strand, within PS417_16675 at 3641.172 kb on - strand, within PS417_16675 at 3641.362 kb on - strand, within PS417_16675 at 3641.404 kb on + strand, within PS417_16675 at 3641.404 kb on + strand, within PS417_16675 at 3641.404 kb on + strand at 3641.405 kb on - strand, within PS417_16675 at 3641.405 kb on - strand, within PS417_16675 at 3641.552 kb on + strand, within PS417_16675 at 3641.552 kb on + strand, within PS417_16675 at 3641.649 kb on - strand at 3641.949 kb on + strand, within PS417_16680 at 3641.949 kb on + strand, within PS417_16680 at 3642.600 kb on + strand, within PS417_16680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,640,256 + -1.2 3,640,256 + -0.2 3,640,320 + -1.3 3,640,320 + -0.1 3,640,320 + -0.9 3,640,321 - +1.8 3,640,321 - +0.3 3,640,321 - -0.4 3,640,372 + PS417_16660 0.13 +0.8 3,640,612 - -2.1 3,640,639 - PS417_16665 0.14 -0.6 3,640,991 + +1.5 3,640,992 - -0.2 3,640,992 - -1.4 3,641,024 - +0.5 3,641,024 - -0.9 3,641,027 + +0.5 3,641,027 + +3.2 3,641,028 - -0.5 3,641,033 + +0.4 3,641,169 - PS417_16675 0.10 -1.3 3,641,169 - PS417_16675 0.10 +1.8 3,641,169 - PS417_16675 0.10 -3.1 3,641,171 + PS417_16675 0.11 -0.7 3,641,171 + PS417_16675 0.11 -1.7 3,641,172 - PS417_16675 0.11 -0.0 3,641,362 - PS417_16675 0.47 +0.1 3,641,404 + PS417_16675 0.55 -2.6 3,641,404 + PS417_16675 0.55 -0.9 3,641,404 + +0.3 3,641,405 - PS417_16675 0.56 -0.5 3,641,405 - PS417_16675 0.56 -1.0 3,641,552 + PS417_16675 0.84 -0.8 3,641,552 + PS417_16675 0.84 +1.8 3,641,649 - -0.0 3,641,949 + PS417_16680 0.24 -0.3 3,641,949 + PS417_16680 0.24 -0.1 3,642,600 + PS417_16680 0.82 -0.0
Or see this region's nucleotide sequence