Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14940 and PS417_14945 are separated by 116 nucleotides PS417_14945 and PS417_14950 are separated by 5 nucleotides PS417_14950 and PS417_14955 are separated by 152 nucleotides
PS417_14940: PS417_14940 - LysR family transcriptional regulator, at 3,262,631 to 3,263,521
_14940
PS417_14945: PS417_14945 - beta-lactamase, at 3,263,638 to 3,265,452
_14945
PS417_14950: PS417_14950 - structural protein MipA, at 3,265,458 to 3,266,261
_14950
PS417_14955: PS417_14955 - hypothetical protein, at 3,266,414 to 3,270,472
_14955
Position (kb)
3263
3264
3265
3266 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3263.044 kb on - strand, within PS417_14940 at 3263.741 kb on - strand at 3264.020 kb on - strand, within PS417_14945 at 3264.020 kb on - strand, within PS417_14945 at 3264.140 kb on - strand, within PS417_14945 at 3264.140 kb on - strand, within PS417_14945 at 3264.211 kb on + strand, within PS417_14945 at 3264.457 kb on + strand at 3264.471 kb on - strand, within PS417_14945 at 3264.488 kb on - strand at 3264.636 kb on + strand, within PS417_14945 at 3264.898 kb on + strand, within PS417_14945 at 3265.065 kb on - strand, within PS417_14945 at 3265.065 kb on - strand, within PS417_14945 at 3265.196 kb on - strand, within PS417_14945 at 3265.309 kb on + strand at 3265.309 kb on + strand at 3265.310 kb on - strand at 3265.310 kb on - strand at 3265.429 kb on - strand at 3265.554 kb on + strand, within PS417_14950 at 3265.840 kb on - strand, within PS417_14950 at 3265.957 kb on - strand, within PS417_14950 at 3266.102 kb on + strand, within PS417_14950 at 3266.251 kb on - strand at 3266.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,263,044 - PS417_14940 0.46 -0.1 3,263,741 - -4.1 3,264,020 - PS417_14945 0.21 -4.4 3,264,020 - PS417_14945 0.21 -0.2 3,264,140 - PS417_14945 0.28 -3.2 3,264,140 - PS417_14945 0.28 -2.6 3,264,211 + PS417_14945 0.32 -3.2 3,264,457 + -1.4 3,264,471 - PS417_14945 0.46 -2.5 3,264,488 - +0.2 3,264,636 + PS417_14945 0.55 -2.2 3,264,898 + PS417_14945 0.69 -2.2 3,265,065 - PS417_14945 0.79 -3.2 3,265,065 - PS417_14945 0.79 +0.8 3,265,196 - PS417_14945 0.86 -2.2 3,265,309 + -1.7 3,265,309 + -3.5 3,265,310 - -4.0 3,265,310 - -2.5 3,265,429 - +0.6 3,265,554 + PS417_14950 0.12 +0.7 3,265,840 - PS417_14950 0.48 +2.0 3,265,957 - PS417_14950 0.62 +0.2 3,266,102 + PS417_14950 0.80 +0.3 3,266,251 - +0.9 3,266,410 - -1.1
Or see this region's nucleotide sequence