Strain Fitness in Pseudomonas simiae WCS417 around PS417_01450

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01440 and PS417_01445 are separated by 289 nucleotidesPS417_01445 and PS417_01450 overlap by 4 nucleotides PS417_01440: PS417_01440 - hypothetical protein, at 320,468 to 321,907 _01440 PS417_01445: PS417_01445 - hypothetical protein, at 322,197 to 322,472 _01445 PS417_01450: PS417_01450 - integrase, at 322,469 to 323,449 _01450 Position (kb) 322 323 324Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 321.503 kb on + strand, within PS417_01440at 321.546 kb on - strand, within PS417_01440at 321.687 kb on + strand, within PS417_01440at 321.688 kb on - strand, within PS417_01440at 321.737 kb on - strand, within PS417_01440at 321.793 kb on - strandat 321.872 kb on - strandat 321.930 kb on - strandat 321.930 kb on - strandat 321.947 kb on - strandat 322.000 kb on + strandat 322.003 kb on + strandat 322.004 kb on - strandat 322.004 kb on - strandat 322.258 kb on + strand, within PS417_01445at 322.258 kb on + strand, within PS417_01445at 322.258 kb on + strand, within PS417_01445at 322.258 kb on + strand, within PS417_01445at 322.259 kb on - strand, within PS417_01445at 322.259 kb on - strand, within PS417_01445at 322.262 kb on - strand, within PS417_01445at 322.264 kb on + strand, within PS417_01445at 322.265 kb on - strand, within PS417_01445at 322.265 kb on - strand, within PS417_01445at 322.265 kb on - strand, within PS417_01445at 322.344 kb on - strand, within PS417_01445at 322.347 kb on + strand, within PS417_01445at 322.347 kb on + strand, within PS417_01445at 322.395 kb on + strand, within PS417_01445at 322.395 kb on + strand, within PS417_01445at 322.396 kb on - strand, within PS417_01445at 322.396 kb on - strand, within PS417_01445at 322.396 kb on - strand, within PS417_01445at 322.396 kb on - strand, within PS417_01445at 322.488 kb on + strandat 322.488 kb on + strandat 322.552 kb on + strandat 322.552 kb on + strandat 322.552 kb on + strandat 322.553 kb on - strandat 322.553 kb on - strandat 322.638 kb on + strand, within PS417_01450at 322.639 kb on - strand, within PS417_01450at 322.639 kb on - strand, within PS417_01450at 322.639 kb on - strand, within PS417_01450at 322.678 kb on + strand, within PS417_01450at 322.760 kb on - strand, within PS417_01450at 322.804 kb on + strand, within PS417_01450at 322.805 kb on - strand, within PS417_01450at 322.805 kb on - strand, within PS417_01450at 322.805 kb on - strand, within PS417_01450at 322.805 kb on - strand, within PS417_01450at 322.916 kb on + strand, within PS417_01450at 322.916 kb on + strand, within PS417_01450at 322.917 kb on - strand, within PS417_01450at 322.917 kb on - strand, within PS417_01450at 323.016 kb on - strand, within PS417_01450at 323.016 kb on - strand, within PS417_01450at 323.118 kb on + strand, within PS417_01450at 323.119 kb on - strand, within PS417_01450at 323.119 kb on - strand, within PS417_01450at 323.159 kb on - strand, within PS417_01450at 323.217 kb on - strand, within PS417_01450at 323.217 kb on - strand, within PS417_01450at 323.217 kb on - strand, within PS417_01450at 323.230 kb on - strand, within PS417_01450at 323.230 kb on - strand, within PS417_01450at 323.363 kb on + strandat 323.364 kb on - strandat 323.364 kb on - strandat 323.364 kb on - strandat 323.480 kb on - strandat 323.584 kb on - strandat 323.584 kb on - strandat 323.850 kb on + strandat 323.850 kb on + strandat 323.943 kb on - strandat 323.974 kb on + strandat 323.974 kb on + strandat 324.101 kb on + strandat 324.146 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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321,503 + PS417_01440 0.72 -1.0
321,546 - PS417_01440 0.75 +0.1
321,687 + PS417_01440 0.85 -0.5
321,688 - PS417_01440 0.85 +0.2
321,737 - PS417_01440 0.88 -0.6
321,793 - -1.0
321,872 - +1.0
321,930 - -1.2
321,930 - -0.5
321,947 - -2.0
322,000 + +0.8
322,003 + +0.3
322,004 - +0.3
322,004 - +1.4
322,258 + PS417_01445 0.22 +0.8
322,258 + PS417_01445 0.22 -0.7
322,258 + PS417_01445 0.22 +0.2
322,258 + PS417_01445 0.22 +0.3
322,259 - PS417_01445 0.22 -0.5
322,259 - PS417_01445 0.22 -1.0
322,262 - PS417_01445 0.24 +0.2
322,264 + PS417_01445 0.24 -0.0
322,265 - PS417_01445 0.25 -1.2
322,265 - PS417_01445 0.25 +1.7
322,265 - PS417_01445 0.25 -0.2
322,344 - PS417_01445 0.53 +0.4
322,347 + PS417_01445 0.54 +0.7
322,347 + PS417_01445 0.54 +1.5
322,395 + PS417_01445 0.72 -1.6
322,395 + PS417_01445 0.72 -0.2
322,396 - PS417_01445 0.72 +0.3
322,396 - PS417_01445 0.72 -0.5
322,396 - PS417_01445 0.72 -2.2
322,396 - PS417_01445 0.72 +0.1
322,488 + -0.1
322,488 + +0.4
322,552 + -1.2
322,552 + -2.8
322,552 + +0.0
322,553 - -1.2
322,553 - -1.0
322,638 + PS417_01450 0.17 +1.8
322,639 - PS417_01450 0.17 +1.0
322,639 - PS417_01450 0.17 -0.6
322,639 - PS417_01450 0.17 -0.5
322,678 + PS417_01450 0.21 +2.8
322,760 - PS417_01450 0.30 -0.2
322,804 + PS417_01450 0.34 +0.9
322,805 - PS417_01450 0.34 +0.3
322,805 - PS417_01450 0.34 +1.3
322,805 - PS417_01450 0.34 +0.0
322,805 - PS417_01450 0.34 +0.1
322,916 + PS417_01450 0.46 +3.0
322,916 + PS417_01450 0.46 +1.1
322,917 - PS417_01450 0.46 -0.2
322,917 - PS417_01450 0.46 +2.4
323,016 - PS417_01450 0.56 +0.9
323,016 - PS417_01450 0.56 +0.2
323,118 + PS417_01450 0.66 -2.6
323,119 - PS417_01450 0.66 +0.0
323,119 - PS417_01450 0.66 +0.8
323,159 - PS417_01450 0.70 +0.3
323,217 - PS417_01450 0.76 -0.4
323,217 - PS417_01450 0.76 -1.3
323,217 - PS417_01450 0.76 +1.4
323,230 - PS417_01450 0.78 -0.4
323,230 - PS417_01450 0.78 +0.8
323,363 + -0.6
323,364 - -0.6
323,364 - -1.3
323,364 - +0.8
323,480 - +0.5
323,584 - +1.5
323,584 - +1.4
323,850 + +4.6
323,850 + -0.3
323,943 - -1.6
323,974 + -2.4
323,974 + +0.2
324,101 + -0.1
324,146 - +0.0

Or see this region's nucleotide sequence