Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14510 and PS417_14515 overlap by 4 nucleotides PS417_14515 and PS417_14520 overlap by 4 nucleotides PS417_14520 and PS417_14525 overlap by 4 nucleotides PS417_14525 and PS417_14530 are separated by 178 nucleotides
PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557
_14510
PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312
_14515
PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908
_14520
PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564
_14525
PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504
_14530
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-1
0
1 at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.472 kb on - strand at 3174.472 kb on - strand at 3174.969 kb on - strand, within PS417_14515 at 3175.309 kb on - strand at 3175.436 kb on - strand, within PS417_14520 at 3175.638 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520 at 3176.376 kb on + strand, within PS417_14525 at 3176.577 kb on + strand at 3176.578 kb on - strand at 3176.626 kb on + strand at 3176.827 kb on - strand, within PS417_14530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,174,471 + -0.0 3,174,471 + -0.1 3,174,471 + +1.2 3,174,472 - -0.5 3,174,472 - -0.4 3,174,969 - PS417_14515 0.55 -0.5 3,175,309 - +0.9 3,175,436 - PS417_14520 0.21 +0.3 3,175,638 - PS417_14520 0.55 -1.3 3,175,655 - PS417_14520 0.58 +0.1 3,175,655 - PS417_14520 0.58 -1.3 3,176,376 + PS417_14525 0.71 -1.7 3,176,577 + -0.9 3,176,578 - -0.2 3,176,626 + -1.1 3,176,827 - PS417_14530 0.11 -0.1
Or see this region's nucleotide sequence