Strain Fitness in Pseudomonas simiae WCS417 around PS417_01350

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01340 and PS417_01345 are separated by 233 nucleotidesPS417_01345 and PS417_01350 are separated by 38 nucleotidesPS417_01350 and PS417_01355 are separated by 411 nucleotides PS417_01340: PS417_01340 - agmatine deiminase, at 291,997 to 293,103 _01340 PS417_01345: PS417_01345 - hypothetical protein, at 293,337 to 293,555 _01345 PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681 _01350 PS417_01355: PS417_01355 - membrane protein, at 296,093 to 297,409 _01355 Position (kb) 293 294 295 296Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 292.723 kb on + strand, within PS417_01340at 292.723 kb on + strand, within PS417_01340at 292.724 kb on - strand, within PS417_01340at 292.798 kb on - strand, within PS417_01340at 292.798 kb on - strand, within PS417_01340at 292.822 kb on + strand, within PS417_01340at 292.878 kb on + strand, within PS417_01340at 292.879 kb on - strand, within PS417_01340at 292.906 kb on + strand, within PS417_01340at 292.907 kb on - strand, within PS417_01340at 292.907 kb on - strand, within PS417_01340at 293.114 kb on - strandat 293.211 kb on - strandat 293.231 kb on + strandat 293.232 kb on - strandat 293.289 kb on + strandat 293.300 kb on + strandat 293.301 kb on - strandat 293.553 kb on + strandat 293.576 kb on - strandat 293.604 kb on + strandat 293.604 kb on + strandat 293.604 kb on + strandat 293.604 kb on + strandat 293.605 kb on - strandat 293.676 kb on + strandat 293.676 kb on + strandat 293.761 kb on - strandat 293.885 kb on + strand, within PS417_01350at 293.968 kb on - strand, within PS417_01350at 293.968 kb on - strand, within PS417_01350at 294.078 kb on + strand, within PS417_01350at 294.079 kb on - strand, within PS417_01350at 294.079 kb on - strand, within PS417_01350at 294.261 kb on + strand, within PS417_01350at 294.261 kb on + strand, within PS417_01350at 294.261 kb on + strand, within PS417_01350at 294.261 kb on + strand, within PS417_01350at 294.262 kb on - strand, within PS417_01350at 294.262 kb on - strand, within PS417_01350at 294.438 kb on + strand, within PS417_01350at 294.438 kb on + strand, within PS417_01350at 294.577 kb on - strand, within PS417_01350at 294.642 kb on + strand, within PS417_01350at 294.646 kb on + strand, within PS417_01350at 294.646 kb on + strand, within PS417_01350at 294.744 kb on + strand, within PS417_01350at 294.745 kb on - strand, within PS417_01350at 294.745 kb on - strand, within PS417_01350at 294.756 kb on + strand, within PS417_01350at 294.756 kb on + strand, within PS417_01350at 294.757 kb on - strand, within PS417_01350at 294.757 kb on - strand, within PS417_01350at 294.760 kb on + strand, within PS417_01350at 294.760 kb on + strand, within PS417_01350at 294.760 kb on + strand, within PS417_01350at 294.761 kb on - strand, within PS417_01350at 294.761 kb on - strand, within PS417_01350at 294.832 kb on - strand, within PS417_01350at 294.873 kb on + strand, within PS417_01350at 294.891 kb on + strand, within PS417_01350at 294.892 kb on - strand, within PS417_01350at 294.892 kb on - strand, within PS417_01350at 294.973 kb on - strand, within PS417_01350at 294.973 kb on - strand, within PS417_01350at 294.973 kb on - strand, within PS417_01350at 295.140 kb on + strand, within PS417_01350at 295.314 kb on + strand, within PS417_01350at 295.314 kb on + strand, within PS417_01350at 295.315 kb on - strand, within PS417_01350at 295.315 kb on - strand, within PS417_01350at 295.345 kb on - strand, within PS417_01350at 295.507 kb on - strandat 295.603 kb on + strandat 295.603 kb on + strandat 295.604 kb on - strandat 295.785 kb on + strandat 295.788 kb on + strandat 295.789 kb on - strandat 295.843 kb on + strandat 295.844 kb on - strandat 295.844 kb on - strandat 295.855 kb on + strandat 295.900 kb on + strandat 295.901 kb on - strandat 295.984 kb on + strandat 296.084 kb on + strandat 296.084 kb on + strandat 296.085 kb on - strandat 296.086 kb on + strandat 296.087 kb on - strandat 296.087 kb on - strandat 296.087 kb on - strandat 296.222 kb on + strandat 296.223 kb on - strandat 296.223 kb on - strandat 296.223 kb on - strandat 296.237 kb on + strand, within PS417_01355at 296.238 kb on - strand, within PS417_01355at 296.349 kb on - strand, within PS417_01355at 296.525 kb on + strand, within PS417_01355at 296.525 kb on + strand, within PS417_01355at 296.561 kb on + strand, within PS417_01355at 296.562 kb on - strandat 296.562 kb on - strand, within PS417_01355at 296.591 kb on + strand, within PS417_01355at 296.592 kb on - strand, within PS417_01355at 296.592 kb on - strand, within PS417_01355at 296.592 kb on - strand, within PS417_01355

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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292,723 + PS417_01340 0.66 -1.5
292,723 + PS417_01340 0.66 -2.1
292,724 - PS417_01340 0.66 -0.4
292,798 - PS417_01340 0.72 -1.2
292,798 - PS417_01340 0.72 +0.0
292,822 + PS417_01340 0.75 +1.2
292,878 + PS417_01340 0.80 -1.0
292,879 - PS417_01340 0.80 -4.7
292,906 + PS417_01340 0.82 -2.2
292,907 - PS417_01340 0.82 -1.8
292,907 - PS417_01340 0.82 -0.6
293,114 - -0.5
293,211 - +0.9
293,231 + +0.1
293,232 - +0.9
293,289 + -0.2
293,300 + -0.8
293,301 - +0.9
293,553 + +1.6
293,576 - +2.0
293,604 + -0.4
293,604 + +0.9
293,604 + -1.9
293,604 + +0.4
293,605 - -0.2
293,676 + +0.1
293,676 + +0.1
293,761 - +1.6
293,885 + PS417_01350 0.14 -0.1
293,968 - PS417_01350 0.18 -0.6
293,968 - PS417_01350 0.18 +0.6
294,078 + PS417_01350 0.23 -0.2
294,079 - PS417_01350 0.23 +0.2
294,079 - PS417_01350 0.23 -0.7
294,261 + PS417_01350 0.32 -1.2
294,261 + PS417_01350 0.32 +1.3
294,261 + PS417_01350 0.32 +0.7
294,261 + PS417_01350 0.32 -0.8
294,262 - PS417_01350 0.32 -1.1
294,262 - PS417_01350 0.32 -1.2
294,438 + PS417_01350 0.40 +3.4
294,438 + PS417_01350 0.40 -1.0
294,577 - PS417_01350 0.47 +0.2
294,642 + PS417_01350 0.50 +0.3
294,646 + PS417_01350 0.50 -0.0
294,646 + PS417_01350 0.50 +0.0
294,744 + PS417_01350 0.55 +1.1
294,745 - PS417_01350 0.55 +0.1
294,745 - PS417_01350 0.55 -0.0
294,756 + PS417_01350 0.56 -0.7
294,756 + PS417_01350 0.56 -0.7
294,757 - PS417_01350 0.56 +0.1
294,757 - PS417_01350 0.56 +0.9
294,760 + PS417_01350 0.56 -0.5
294,760 + PS417_01350 0.56 +1.3
294,760 + PS417_01350 0.56 +1.8
294,761 - PS417_01350 0.56 +0.4
294,761 - PS417_01350 0.56 -2.4
294,832 - PS417_01350 0.59 -0.3
294,873 + PS417_01350 0.61 +1.2
294,891 + PS417_01350 0.62 +0.5
294,892 - PS417_01350 0.62 -1.2
294,892 - PS417_01350 0.62 -0.8
294,973 - PS417_01350 0.66 -1.7
294,973 - PS417_01350 0.66 +1.2
294,973 - PS417_01350 0.66 +0.0
295,140 + PS417_01350 0.74 +2.4
295,314 + PS417_01350 0.82 -0.7
295,314 + PS417_01350 0.82 +0.3
295,315 - PS417_01350 0.82 -0.1
295,315 - PS417_01350 0.82 +2.7
295,345 - PS417_01350 0.84 +0.8
295,507 - +0.7
295,603 + -0.2
295,603 + -0.6
295,604 - -0.1
295,785 + -0.5
295,788 + +0.2
295,789 - -0.4
295,843 + +0.1
295,844 - -1.2
295,844 - +2.0
295,855 + -0.6
295,900 + +0.2
295,901 - -0.6
295,984 + -0.0
296,084 + -1.0
296,084 + +0.8
296,085 - +0.1
296,086 + -0.5
296,087 - +0.3
296,087 - -0.6
296,087 - -2.1
296,222 + -2.6
296,223 - -0.2
296,223 - +0.6
296,223 - +0.9
296,237 + PS417_01355 0.11 -0.8
296,238 - PS417_01355 0.11 -0.1
296,349 - PS417_01355 0.19 -0.2
296,525 + PS417_01355 0.33 -1.6
296,525 + PS417_01355 0.33 -0.2
296,561 + PS417_01355 0.36 +1.4
296,562 - -0.6
296,562 - PS417_01355 0.36 -1.4
296,591 + PS417_01355 0.38 -2.0
296,592 - PS417_01355 0.38 +0.1
296,592 - PS417_01355 0.38 +0.2
296,592 - PS417_01355 0.38 -2.8

Or see this region's nucleotide sequence