Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01335 and PS417_01340 are separated by 121 nucleotides PS417_01340 and PS417_01345 are separated by 233 nucleotides PS417_01345 and PS417_01350 are separated by 38 nucleotides
PS417_01335: PS417_01335 - hypothetical protein, at 288,975 to 291,875
_01335
PS417_01340: PS417_01340 - agmatine deiminase, at 291,997 to 293,103
_01340
PS417_01345: PS417_01345 - hypothetical protein, at 293,337 to 293,555
_01345
PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681
_01350
Position (kb) 291
292
293
294 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 291.136 kb on - strand, within PS417_01335 at 291.216 kb on + strand, within PS417_01335 at 291.217 kb on - strand, within PS417_01335 at 291.267 kb on + strand, within PS417_01335 at 291.268 kb on - strand, within PS417_01335 at 291.332 kb on + strand, within PS417_01335 at 291.386 kb on + strand, within PS417_01335 at 291.386 kb on + strand, within PS417_01335 at 291.387 kb on - strand, within PS417_01335 at 291.387 kb on - strand at 291.387 kb on - strand, within PS417_01335 at 291.435 kb on - strand, within PS417_01335 at 291.475 kb on - strand, within PS417_01335 at 291.871 kb on + strand at 291.873 kb on + strand at 291.873 kb on + strand at 291.874 kb on - strand at 291.874 kb on - strand at 292.723 kb on + strand, within PS417_01340 at 292.723 kb on + strand, within PS417_01340 at 292.724 kb on - strand, within PS417_01340 at 292.798 kb on - strand, within PS417_01340 at 292.798 kb on - strand, within PS417_01340 at 292.822 kb on + strand, within PS417_01340 at 292.878 kb on + strand, within PS417_01340 at 292.879 kb on - strand, within PS417_01340 at 292.906 kb on + strand, within PS417_01340 at 292.907 kb on - strand, within PS417_01340 at 292.907 kb on - strand, within PS417_01340 at 293.114 kb on - strand at 293.211 kb on - strand at 293.231 kb on + strand at 293.232 kb on - strand at 293.289 kb on + strand at 293.300 kb on + strand at 293.301 kb on - strand at 293.553 kb on + strand at 293.576 kb on - strand at 293.604 kb on + strand at 293.604 kb on + strand at 293.604 kb on + strand at 293.604 kb on + strand at 293.605 kb on - strand at 293.676 kb on + strand at 293.676 kb on + strand at 293.761 kb on - strand at 293.885 kb on + strand, within PS417_01350 at 293.968 kb on - strand, within PS417_01350 at 293.968 kb on - strand, within PS417_01350 at 294.078 kb on + strand, within PS417_01350 at 294.079 kb on - strand, within PS417_01350 at 294.079 kb on - strand, within PS417_01350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 291,136 - PS417_01335 0.74 -2.3 291,216 + PS417_01335 0.77 -0.1 291,217 - PS417_01335 0.77 +0.9 291,267 + PS417_01335 0.79 +0.1 291,268 - PS417_01335 0.79 +0.2 291,332 + PS417_01335 0.81 +0.1 291,386 + PS417_01335 0.83 +0.8 291,386 + PS417_01335 0.83 +1.2 291,387 - PS417_01335 0.83 +0.3 291,387 - -0.6 291,387 - PS417_01335 0.83 -0.2 291,435 - PS417_01335 0.85 +0.6 291,475 - PS417_01335 0.86 +0.7 291,871 + -0.4 291,873 + +0.2 291,873 + +0.0 291,874 - +1.0 291,874 - +0.1 292,723 + PS417_01340 0.66 -1.5 292,723 + PS417_01340 0.66 -2.1 292,724 - PS417_01340 0.66 -0.4 292,798 - PS417_01340 0.72 -1.2 292,798 - PS417_01340 0.72 +0.0 292,822 + PS417_01340 0.75 +1.2 292,878 + PS417_01340 0.80 -1.0 292,879 - PS417_01340 0.80 -4.7 292,906 + PS417_01340 0.82 -2.2 292,907 - PS417_01340 0.82 -1.8 292,907 - PS417_01340 0.82 -0.6 293,114 - -0.5 293,211 - +0.9 293,231 + +0.1 293,232 - +0.9 293,289 + -0.2 293,300 + -0.8 293,301 - +0.9 293,553 + +1.6 293,576 - +2.0 293,604 + -0.4 293,604 + +0.9 293,604 + -1.9 293,604 + +0.4 293,605 - -0.2 293,676 + +0.1 293,676 + +0.1 293,761 - +1.6 293,885 + PS417_01350 0.14 -0.1 293,968 - PS417_01350 0.18 -0.6 293,968 - PS417_01350 0.18 +0.6 294,078 + PS417_01350 0.23 -0.2 294,079 - PS417_01350 0.23 +0.2 294,079 - PS417_01350 0.23 -0.7
Or see this region's nucleotide sequence