Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12065 and PS417_12070 are separated by 283 nucleotides PS417_12070 and PS417_12075 are separated by 63 nucleotides PS417_12075 and PS417_12080 are separated by 263 nucleotides
PS417_12065: PS417_12065 - xylitol ABC transporter, ATPase component (from data) , at 2,620,222 to 2,621,709
_12065
PS417_12070: PS417_12070 - ribose 5-phosphate isomerase, at 2,621,993 to 2,622,454
_12070
PS417_12075: PS417_12075 - DNA-binding protein, at 2,622,518 to 2,623,498
_12075
PS417_12080: PS417_12080 - membrane protein, at 2,623,762 to 2,625,324
_12080
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2621.855 kb on + strand at 2621.855 kb on + strand at 2621.855 kb on + strand at 2621.856 kb on - strand at 2622.461 kb on - strand at 2622.530 kb on - strand at 2622.581 kb on + strand at 2622.581 kb on + strand at 2622.581 kb on + strand at 2622.582 kb on - strand at 2622.582 kb on - strand at 2622.584 kb on + strand at 2622.585 kb on - strand at 2622.834 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.981 kb on - strand, within PS417_12075 at 2622.997 kb on + strand, within PS417_12075 at 2622.997 kb on + strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2622.998 kb on - strand, within PS417_12075 at 2623.046 kb on + strand, within PS417_12075 at 2623.046 kb on + strand, within PS417_12075 at 2623.047 kb on - strand, within PS417_12075 at 2623.183 kb on + strand, within PS417_12075 at 2623.184 kb on - strand, within PS417_12075 at 2623.262 kb on + strand, within PS417_12075 at 2623.429 kb on + strand at 2623.429 kb on + strand at 2623.562 kb on + strand at 2623.563 kb on - strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.641 kb on + strand at 2623.642 kb on - strand at 2623.953 kb on + strand, within PS417_12080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,621,855 + +0.7 2,621,855 + -0.8 2,621,855 + -0.5 2,621,856 - +2.1 2,622,461 - -0.6 2,622,530 - +0.1 2,622,581 + -1.0 2,622,581 + -0.5 2,622,581 + -0.4 2,622,582 - +0.4 2,622,582 - +0.2 2,622,584 + +1.3 2,622,585 - -0.4 2,622,834 - PS417_12075 0.32 -2.3 2,622,981 - PS417_12075 0.47 -0.8 2,622,981 - PS417_12075 0.47 +0.7 2,622,981 - PS417_12075 0.47 -1.9 2,622,997 + PS417_12075 0.49 +0.2 2,622,997 + PS417_12075 0.49 -0.2 2,622,998 - PS417_12075 0.49 -2.4 2,622,998 - PS417_12075 0.49 -1.1 2,622,998 - PS417_12075 0.49 -1.3 2,623,046 + PS417_12075 0.54 +0.6 2,623,046 + PS417_12075 0.54 -0.4 2,623,047 - PS417_12075 0.54 -0.5 2,623,183 + PS417_12075 0.68 -0.4 2,623,184 - PS417_12075 0.68 -3.5 2,623,262 + PS417_12075 0.76 -0.8 2,623,429 + +1.5 2,623,429 + -1.2 2,623,562 + -0.8 2,623,563 - -0.8 2,623,641 + +0.8 2,623,641 + -0.5 2,623,641 + -1.1 2,623,641 + +1.1 2,623,641 + -0.3 2,623,642 - -1.3 2,623,953 + PS417_12080 0.12 -0.0
Or see this region's nucleotide sequence