Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01140 and PS417_01145 overlap by 4 nucleotides PS417_01145 and PS417_01150 are separated by 16 nucleotides PS417_01150 and PS417_01155 are separated by 241 nucleotides
PS417_01140: PS417_01140 - taurine ABC transporter permease, at 250,683 to 251,519
_01140
PS417_01145: PS417_01145 - taurine transporter ATP-binding subunit, at 251,516 to 252,295
_01145
PS417_01150: PS417_01150 - taurine ABC transporter substrate-binding protein, at 252,312 to 253,289
_01150
PS417_01155: PS417_01155 - glutamate--cysteine ligase, at 253,531 to 255,114
_01155
Position (kb) 252
253
254 Strain fitness (log2 ratio) -2
-1
0
1
2 at 251.623 kb on - strand, within PS417_01145 at 252.237 kb on - strand at 252.284 kb on + strand at 252.284 kb on + strand at 252.303 kb on - strand at 252.340 kb on + strand at 252.340 kb on + strand at 252.341 kb on - strand at 252.341 kb on - strand at 252.349 kb on + strand at 252.419 kb on + strand, within PS417_01150 at 252.584 kb on - strand, within PS417_01150 at 252.757 kb on + strand, within PS417_01150 at 252.758 kb on - strand at 252.758 kb on - strand, within PS417_01150 at 252.758 kb on - strand, within PS417_01150 at 252.825 kb on + strand, within PS417_01150 at 253.185 kb on + strand, within PS417_01150 at 253.532 kb on + strand at 253.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 251,623 - PS417_01145 0.14 +1.1 252,237 - -1.0 252,284 + -0.0 252,284 + -0.6 252,303 - +0.0 252,340 + -0.7 252,340 + -1.9 252,341 - -1.5 252,341 - +0.2 252,349 + +1.8 252,419 + PS417_01150 0.11 -0.5 252,584 - PS417_01150 0.28 -1.5 252,757 + PS417_01150 0.46 +0.1 252,758 - +1.8 252,758 - PS417_01150 0.46 -0.8 252,758 - PS417_01150 0.46 -1.6 252,825 + PS417_01150 0.52 -0.3 253,185 + PS417_01150 0.89 -0.2 253,532 + +2.0 253,533 - +0.3
Or see this region's nucleotide sequence