Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01110 and PS417_01115 are separated by 165 nucleotides PS417_01115 and PS417_01120 are separated by 270 nucleotides
PS417_01110: PS417_01110 - cysteine desulfhydrase, at 242,694 to 243,686
_01110
PS417_01115: PS417_01115 - serine acetyltransferase, at 243,852 to 244,778
_01115
PS417_01120: PS417_01120 - choline transporter, at 245,049 to 247,049
_01120
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 242.911 kb on + strand, within PS417_01110 at 242.911 kb on + strand, within PS417_01110 at 243.178 kb on - strand, within PS417_01110 at 243.181 kb on + strand, within PS417_01110 at 243.182 kb on - strand, within PS417_01110 at 243.227 kb on - strand, within PS417_01110 at 243.358 kb on + strand, within PS417_01110 at 243.358 kb on + strand, within PS417_01110 at 243.474 kb on + strand, within PS417_01110 at 243.517 kb on + strand, within PS417_01110 at 243.518 kb on - strand, within PS417_01110 at 243.580 kb on + strand, within PS417_01110 at 243.580 kb on + strand, within PS417_01110 at 243.580 kb on + strand, within PS417_01110 at 243.581 kb on - strand, within PS417_01110 at 243.581 kb on - strand, within PS417_01110 at 243.691 kb on - strand at 243.704 kb on - strand at 243.704 kb on - strand at 243.707 kb on + strand at 243.708 kb on - strand at 243.708 kb on - strand at 243.720 kb on + strand at 243.721 kb on - strand at 243.818 kb on + strand at 243.819 kb on - strand at 243.849 kb on - strand at 243.881 kb on - strand at 244.075 kb on - strand, within PS417_01115 at 244.531 kb on - strand, within PS417_01115 at 244.635 kb on + strand, within PS417_01115 at 244.734 kb on - strand at 244.855 kb on + strand at 244.870 kb on + strand at 244.870 kb on + strand at 244.871 kb on - strand at 244.875 kb on + strand at 244.875 kb on + strand at 244.876 kb on - strand at 244.876 kb on - strand at 244.883 kb on - strand at 244.886 kb on + strand at 244.924 kb on + strand at 244.925 kb on - strand at 244.991 kb on + strand at 244.992 kb on - strand at 244.992 kb on - strand at 245.013 kb on + strand at 245.014 kb on - strand at 245.139 kb on - strand at 245.141 kb on + strand at 245.142 kb on - strand at 245.223 kb on + strand at 245.223 kb on + strand at 245.223 kb on + strand at 245.223 kb on + strand at 245.224 kb on - strand at 245.244 kb on + strand at 245.291 kb on - strand, within PS417_01120 at 245.291 kb on - strand, within PS417_01120 at 245.328 kb on + strand, within PS417_01120 at 245.655 kb on - strand, within PS417_01120 at 245.718 kb on + strand, within PS417_01120 at 245.719 kb on - strand, within PS417_01120 at 245.719 kb on - strand, within PS417_01120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 242,911 + PS417_01110 0.22 -2.4 242,911 + PS417_01110 0.22 +0.5 243,178 - PS417_01110 0.49 +0.1 243,181 + PS417_01110 0.49 -0.3 243,182 - PS417_01110 0.49 -2.4 243,227 - PS417_01110 0.54 -1.5 243,358 + PS417_01110 0.67 +0.5 243,358 + PS417_01110 0.67 +0.4 243,474 + PS417_01110 0.79 +2.0 243,517 + PS417_01110 0.83 +0.2 243,518 - PS417_01110 0.83 -1.0 243,580 + PS417_01110 0.89 +0.9 243,580 + PS417_01110 0.89 +0.2 243,580 + PS417_01110 0.89 +0.6 243,581 - PS417_01110 0.89 +0.2 243,581 - PS417_01110 0.89 +0.8 243,691 - -0.9 243,704 - -0.1 243,704 - +0.0 243,707 + -1.0 243,708 - -0.2 243,708 - +0.2 243,720 + -0.6 243,721 - -0.6 243,818 + -0.8 243,819 - +3.3 243,849 - -0.6 243,881 - +0.2 244,075 - PS417_01115 0.24 +1.1 244,531 - PS417_01115 0.73 -1.5 244,635 + PS417_01115 0.84 -1.6 244,734 - +1.0 244,855 + +1.6 244,870 + +1.5 244,870 + -1.4 244,871 - +0.3 244,875 + -2.0 244,875 + -0.3 244,876 - +0.5 244,876 - -0.0 244,883 - +0.5 244,886 + -0.3 244,924 + -0.7 244,925 - -0.6 244,991 + +0.8 244,992 - +1.5 244,992 - +0.6 245,013 + -0.5 245,014 - -0.7 245,139 - -0.7 245,141 + -0.6 245,142 - +2.1 245,223 + -1.0 245,223 + -1.0 245,223 + -0.5 245,223 + -1.0 245,224 - -1.1 245,244 + +0.3 245,291 - PS417_01120 0.12 -1.0 245,291 - PS417_01120 0.12 +0.6 245,328 + PS417_01120 0.14 -0.0 245,655 - PS417_01120 0.30 +0.9 245,718 + PS417_01120 0.33 +0.9 245,719 - PS417_01120 0.33 +1.8 245,719 - PS417_01120 0.33 -1.2
Or see this region's nucleotide sequence