Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10805 and PS417_10810 are separated by 157 nucleotides PS417_10810 and PS417_10815 are separated by 298 nucleotides PS417_10815 and PS417_10820 overlap by 4 nucleotides
PS417_10805: PS417_10805 - chemotaxis protein CheY, at 2,308,680 to 2,310,905
_10805
PS417_10810: PS417_10810 - hypothetical protein, at 2,311,063 to 2,311,332
_10810
PS417_10815: PS417_10815 - TetR family transcriptional regulator, at 2,311,631 to 2,312,302
_10815
PS417_10820: PS417_10820 - N-methylproline demethylase, at 2,312,299 to 2,314,335
_10820
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2310.926 kb on + strand at 2310.947 kb on + strand at 2311.005 kb on + strand at 2311.005 kb on + strand at 2311.107 kb on + strand, within PS417_10810 at 2311.107 kb on + strand at 2311.108 kb on - strand, within PS417_10810 at 2311.259 kb on + strand, within PS417_10810 at 2311.340 kb on - strand at 2311.457 kb on - strand at 2311.550 kb on + strand at 2311.550 kb on + strand at 2311.560 kb on + strand at 2311.560 kb on + strand at 2311.561 kb on - strand at 2311.592 kb on + strand at 2311.593 kb on - strand at 2311.904 kb on - strand, within PS417_10815 at 2312.120 kb on + strand, within PS417_10815 at 2312.120 kb on + strand, within PS417_10815 at 2312.121 kb on - strand, within PS417_10815 at 2312.140 kb on + strand, within PS417_10815 at 2312.331 kb on - strand at 2312.490 kb on - strand at 2312.490 kb on - strand at 2313.098 kb on - strand, within PS417_10820 at 2313.246 kb on + strand, within PS417_10820 at 2313.251 kb on + strand, within PS417_10820 at 2313.269 kb on + strand, within PS417_10820 at 2313.269 kb on + strand, within PS417_10820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,310,926 + +0.1 2,310,947 + +1.3 2,311,005 + +0.6 2,311,005 + -2.6 2,311,107 + PS417_10810 0.16 -1.6 2,311,107 + -0.2 2,311,108 - PS417_10810 0.17 +3.9 2,311,259 + PS417_10810 0.73 +0.2 2,311,340 - -2.5 2,311,457 - -0.5 2,311,550 + +1.7 2,311,550 + +0.8 2,311,560 + +0.7 2,311,560 + -0.2 2,311,561 - -1.2 2,311,592 + +0.5 2,311,593 - -0.5 2,311,904 - PS417_10815 0.41 -0.7 2,312,120 + PS417_10815 0.73 +0.1 2,312,120 + PS417_10815 0.73 -2.0 2,312,121 - PS417_10815 0.73 -0.3 2,312,140 + PS417_10815 0.76 -0.1 2,312,331 - -1.1 2,312,490 - -0.8 2,312,490 - -0.5 2,313,098 - PS417_10820 0.39 +0.1 2,313,246 + PS417_10820 0.46 -1.8 2,313,251 + PS417_10820 0.47 -0.8 2,313,269 + PS417_10820 0.48 +0.7 2,313,269 + PS417_10820 0.48 +0.1
Or see this region's nucleotide sequence