Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10580 and PS417_10585 are separated by 11 nucleotides PS417_10585 and PS417_10590 are separated by 153 nucleotides PS417_10590 and PS417_10595 are separated by 77 nucleotides
PS417_10580: PS417_10580 - glutamine synthetase, at 2,259,507 to 2,260,853
_10580
PS417_10585: PS417_10585 - Cro/Cl family transcriptional regulator, at 2,260,865 to 2,261,422
_10585
PS417_10590: PS417_10590 - hypothetical protein, at 2,261,576 to 2,261,884
_10590
PS417_10595: PS417_10595 - MFS transporter, at 2,261,962 to 2,263,155
_10595
Position (kb)
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2260.627 kb on + strand at 2260.783 kb on + strand at 2260.866 kb on + strand at 2260.866 kb on + strand at 2260.867 kb on - strand at 2260.873 kb on - strand at 2261.154 kb on + strand, within PS417_10585 at 2261.155 kb on - strand, within PS417_10585 at 2261.218 kb on - strand, within PS417_10585 at 2261.223 kb on + strand, within PS417_10585 at 2261.224 kb on - strand, within PS417_10585 at 2261.912 kb on + strand at 2261.912 kb on + strand at 2261.913 kb on - strand at 2261.939 kb on + strand at 2261.940 kb on - strand at 2261.976 kb on - strand at 2261.976 kb on - strand at 2262.068 kb on - strand at 2262.104 kb on + strand, within PS417_10595 at 2262.104 kb on + strand, within PS417_10595 at 2262.178 kb on - strand, within PS417_10595 at 2262.255 kb on + strand, within PS417_10595 at 2262.255 kb on + strand, within PS417_10595 at 2262.268 kb on + strand, within PS417_10595 at 2262.383 kb on - strand, within PS417_10595 at 2262.383 kb on - strand, within PS417_10595 at 2262.673 kb on + strand, within PS417_10595 at 2262.706 kb on + strand, within PS417_10595 at 2262.706 kb on + strand, within PS417_10595 at 2262.706 kb on + strand, within PS417_10595 at 2262.707 kb on - strand, within PS417_10595 at 2262.707 kb on - strand, within PS417_10595 at 2262.707 kb on - strand, within PS417_10595 at 2262.707 kb on - strand, within PS417_10595 at 2262.707 kb on - strand, within PS417_10595 at 2262.793 kb on + strand, within PS417_10595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,260,627 + +1.4 2,260,783 + -0.4 2,260,866 + +0.8 2,260,866 + +0.4 2,260,867 - -0.8 2,260,873 - +0.1 2,261,154 + PS417_10585 0.52 -0.1 2,261,155 - PS417_10585 0.52 +0.2 2,261,218 - PS417_10585 0.63 -0.4 2,261,223 + PS417_10585 0.64 -0.3 2,261,224 - PS417_10585 0.64 +0.6 2,261,912 + +0.6 2,261,912 + -2.4 2,261,913 - +0.0 2,261,939 + -0.8 2,261,940 - +0.3 2,261,976 - -0.2 2,261,976 - -0.5 2,262,068 - +1.7 2,262,104 + PS417_10595 0.12 +1.7 2,262,104 + PS417_10595 0.12 -0.2 2,262,178 - PS417_10595 0.18 -0.1 2,262,255 + PS417_10595 0.25 -1.8 2,262,255 + PS417_10595 0.25 -1.0 2,262,268 + PS417_10595 0.26 +0.1 2,262,383 - PS417_10595 0.35 +1.4 2,262,383 - PS417_10595 0.35 -1.0 2,262,673 + PS417_10595 0.60 +1.1 2,262,706 + PS417_10595 0.62 -0.4 2,262,706 + PS417_10595 0.62 +2.0 2,262,706 + PS417_10595 0.62 +0.1 2,262,707 - PS417_10595 0.62 +0.7 2,262,707 - PS417_10595 0.62 -0.5 2,262,707 - PS417_10595 0.62 +2.2 2,262,707 - PS417_10595 0.62 +0.0 2,262,707 - PS417_10595 0.62 -1.5 2,262,793 + PS417_10595 0.70 +0.1
Or see this region's nucleotide sequence