Strain Fitness in Pseudomonas simiae WCS417 around PS417_01050

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01045 and PS417_01050 overlap by 4 nucleotidesPS417_01050 and PS417_01055 are separated by 118 nucleotides PS417_01045: PS417_01045 - RND transporter, at 227,259 to 228,329 _01045 PS417_01050: PS417_01050 - ACR family transporter, at 228,326 to 231,391 _01050 PS417_01055: PS417_01055 - methyltransferase, at 231,510 to 232,136 _01055 Position (kb) 228 229 230 231 232Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 227.583 kb on + strand, within PS417_01045at 227.583 kb on + strand, within PS417_01045at 227.583 kb on + strand, within PS417_01045at 227.584 kb on - strand, within PS417_01045at 227.584 kb on - strandat 228.384 kb on - strandat 228.423 kb on - strandat 228.573 kb on - strandat 228.573 kb on - strandat 228.751 kb on - strand, within PS417_01050at 228.751 kb on - strand, within PS417_01050at 228.764 kb on + strand, within PS417_01050at 228.893 kb on + strand, within PS417_01050at 229.094 kb on + strand, within PS417_01050at 229.130 kb on + strand, within PS417_01050at 229.130 kb on + strand, within PS417_01050at 229.131 kb on - strand, within PS417_01050at 229.387 kb on + strand, within PS417_01050at 229.469 kb on + strand, within PS417_01050at 229.480 kb on + strand, within PS417_01050at 229.481 kb on - strand, within PS417_01050at 229.616 kb on + strand, within PS417_01050at 229.616 kb on + strand, within PS417_01050at 229.617 kb on - strand, within PS417_01050at 229.617 kb on - strand, within PS417_01050at 229.617 kb on - strand, within PS417_01050at 229.709 kb on + strand, within PS417_01050at 230.105 kb on + strand, within PS417_01050at 230.105 kb on + strand, within PS417_01050at 230.105 kb on + strand, within PS417_01050at 230.106 kb on - strand, within PS417_01050at 230.106 kb on - strand, within PS417_01050at 230.106 kb on - strand, within PS417_01050at 230.191 kb on + strand, within PS417_01050at 230.270 kb on + strand, within PS417_01050at 230.270 kb on + strand, within PS417_01050at 230.271 kb on - strand, within PS417_01050at 230.271 kb on - strand, within PS417_01050at 230.271 kb on - strand, within PS417_01050at 230.414 kb on + strand, within PS417_01050at 230.415 kb on - strand, within PS417_01050at 230.415 kb on - strand, within PS417_01050at 230.415 kb on - strand, within PS417_01050at 230.557 kb on + strand, within PS417_01050at 230.619 kb on + strand, within PS417_01050at 230.774 kb on - strand, within PS417_01050at 230.837 kb on + strand, within PS417_01050at 231.128 kb on + strandat 231.143 kb on + strandat 231.145 kb on + strandat 231.146 kb on - strandat 231.270 kb on - strandat 231.333 kb on - strandat 231.345 kb on - strandat 231.436 kb on + strandat 231.437 kb on - strandat 231.437 kb on - strandat 231.437 kb on - strandat 231.438 kb on + strandat 231.438 kb on + strandat 231.438 kb on + strandat 231.438 kb on + strandat 231.439 kb on - strandat 231.439 kb on - strandat 231.439 kb on - strandat 231.439 kb on - strandat 231.511 kb on + strandat 231.519 kb on - strandat 231.574 kb on + strand, within PS417_01055at 231.577 kb on + strand, within PS417_01055at 231.577 kb on + strand, within PS417_01055at 231.577 kb on + strand, within PS417_01055at 231.578 kb on - strand, within PS417_01055at 231.578 kb on - strand, within PS417_01055at 231.992 kb on + strand, within PS417_01055at 232.082 kb on - strandat 232.166 kb on + strandat 232.166 kb on + strandat 232.166 kb on + strandat 232.166 kb on + strandat 232.167 kb on - strandat 232.167 kb on - strandat 232.167 kb on - strandat 232.167 kb on - strandat 232.167 kb on - strandat 232.168 kb on + strandat 232.168 kb on + strandat 232.168 kb on + strandat 232.169 kb on - strandat 232.244 kb on - strandat 232.284 kb on + strandat 232.284 kb on + strandat 232.285 kb on - strandat 232.285 kb on - strandat 232.285 kb on - strandat 232.302 kb on + strandat 232.313 kb on + strandat 232.313 kb on + strandat 232.314 kb on - strandat 232.314 kb on - strandat 232.315 kb on + strandat 232.315 kb on + strandat 232.316 kb on - strandat 232.316 kb on - strandat 232.321 kb on + strandat 232.321 kb on + strandat 232.349 kb on + strandat 232.349 kb on + strandat 232.350 kb on - strandat 232.350 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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227,583 + PS417_01045 0.30 +0.1
227,583 + PS417_01045 0.30 -1.1
227,583 + PS417_01045 0.30 +0.1
227,584 - PS417_01045 0.30 +1.5
227,584 - +0.8
228,384 - -0.8
228,423 - -0.4
228,573 - -1.7
228,573 - -0.4
228,751 - PS417_01050 0.14 -2.1
228,751 - PS417_01050 0.14 -1.1
228,764 + PS417_01050 0.14 -0.6
228,893 + PS417_01050 0.18 -1.9
229,094 + PS417_01050 0.25 -0.1
229,130 + PS417_01050 0.26 -0.5
229,130 + PS417_01050 0.26 -1.0
229,131 - PS417_01050 0.26 -0.3
229,387 + PS417_01050 0.35 +0.3
229,469 + PS417_01050 0.37 -1.2
229,480 + PS417_01050 0.38 -0.0
229,481 - PS417_01050 0.38 +0.1
229,616 + PS417_01050 0.42 -1.2
229,616 + PS417_01050 0.42 -0.6
229,617 - PS417_01050 0.42 -0.0
229,617 - PS417_01050 0.42 -0.2
229,617 - PS417_01050 0.42 -0.2
229,709 + PS417_01050 0.45 +0.2
230,105 + PS417_01050 0.58 -2.4
230,105 + PS417_01050 0.58 -1.1
230,105 + PS417_01050 0.58 -0.2
230,106 - PS417_01050 0.58 +1.6
230,106 - PS417_01050 0.58 -0.6
230,106 - PS417_01050 0.58 +0.6
230,191 + PS417_01050 0.61 +1.0
230,270 + PS417_01050 0.63 -0.2
230,270 + PS417_01050 0.63 +0.0
230,271 - PS417_01050 0.63 -1.9
230,271 - PS417_01050 0.63 -1.4
230,271 - PS417_01050 0.63 -0.5
230,414 + PS417_01050 0.68 +2.8
230,415 - PS417_01050 0.68 +1.0
230,415 - PS417_01050 0.68 -1.2
230,415 - PS417_01050 0.68 +0.3
230,557 + PS417_01050 0.73 -1.6
230,619 + PS417_01050 0.75 -0.8
230,774 - PS417_01050 0.80 -0.0
230,837 + PS417_01050 0.82 +0.1
231,128 + +0.3
231,143 + -0.1
231,145 + -0.4
231,146 - -0.6
231,270 - +0.2
231,333 - -0.3
231,345 - -0.6
231,436 + +0.7
231,437 - -0.2
231,437 - -1.2
231,437 - +0.5
231,438 + -2.4
231,438 + -0.3
231,438 + +0.4
231,438 + -0.8
231,439 - -0.2
231,439 - +0.6
231,439 - +0.1
231,439 - +0.3
231,511 + -0.2
231,519 - +2.8
231,574 + PS417_01055 0.10 +0.5
231,577 + PS417_01055 0.11 -0.4
231,577 + PS417_01055 0.11 -0.2
231,577 + PS417_01055 0.11 -0.2
231,578 - PS417_01055 0.11 -1.5
231,578 - PS417_01055 0.11 -0.7
231,992 + PS417_01055 0.77 +0.1
232,082 - +0.8
232,166 + +0.0
232,166 + +0.6
232,166 + -0.0
232,166 + -1.6
232,167 - -0.1
232,167 - -0.7
232,167 - +0.9
232,167 - +1.8
232,167 - -0.3
232,168 + -1.6
232,168 + -0.3
232,168 + +0.8
232,169 - -0.8
232,244 - +0.9
232,284 + +0.5
232,284 + -0.1
232,285 - -1.5
232,285 - +0.1
232,285 - -0.3
232,302 + +0.2
232,313 + -0.4
232,313 + -1.1
232,314 - -0.3
232,314 - -0.8
232,315 + -0.7
232,315 + -0.2
232,316 - -0.2
232,316 - -2.9
232,321 + -0.1
232,321 + +1.4
232,349 + -1.0
232,349 + -0.6
232,350 - -0.0
232,350 - +0.4

Or see this region's nucleotide sequence